Biologically Active Metabolites Produced by the Basidiomycete Quambalaria cyanescens

Stodůlková, Eva; Cı́sařová, Ivana; Kolařík, Miroslav; Chudíčková, Milada; Novák, Petr; Man, Petr; Kuzma, Marek; Pavlů, Barbora; Černý, Jan; Flieger, Miroslav

doi:10.1371/journal.pone.0118913

Cited by 21 publications

(38 citation statements)

References 31 publications

(36 reference statements)

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“…Stodulkova et al . [ 13 ] described a novel active metabolite naphthoquinone (quambalarine A) with broad antifungal and antibacterial activity from Q . cyanescens .…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a Rare Fungus, Quambalaria cyanescens, Isolated from the Peritoneal Fluid of a Patient after Nocturnal Intermittent Peritoneal Dialysis

et al. 2015

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Peritonitis is the leading complication of peritoneal dialysis, which is primarily caused by bacteria rather than fungi. Peritonitis is responsible for approximately 18% of the infection-related mortality in peritoneal dialysis patients. In this paper, we report the isolation of a rare fungus, Quambalaria cyanescens, from the peritoneal fluid of a man after he switched from continuous ambulatory peritoneal dialysis to nocturnal intermittent peritoneal dialysis. Based on the morphological examination and multigene phylogeny, the clinical isolate was confirmed as Q. cyanescens. This pathogen exhibited low sensitivity to all tested echinocandins and 5-flucytosine. Interestingly, morphological characterization revealed that Q. cyanescens UM 1095 produced different pigments at low temperatures (25°C and 30°C) on various culture media. It is important to monitor the emergence of this rare fungus as a potential human pathogen in the tropics. This study provides insight into Q. cyanescens UM 1095 phenotype profiles using a Biolog phenotypic microarray (PM). Of the 760 nutrient sources tested, Q. cyanescens UM 1095 utilized 42 compounds, and the fungus can adapt to a broad range of osmotic and acidic environments. To our knowledge, this is the first report of the isolation of Q. cyanescens from peritoneal fluid, revealing this rare fungus as a potential human pathogen that may be misidentified using conventional methods. The detailed morphological, molecular and phenotypic characterization of Q. cyanescens UM 1095 provides the basis for future studies on its biology, lifestyle, and potential pathogenicity.

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“…Stodulkova et al . [ 13 ] described a novel active metabolite naphthoquinone (quambalarine A) with broad antifungal and antibacterial activity from Q . cyanescens .…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a Rare Fungus, Quambalaria cyanescens, Isolated from the Peritoneal Fluid of a Patient after Nocturnal Intermittent Peritoneal Dialysis

et al. 2015

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“…This assay is based on the dye CellROX Green which is a fluorogenic probe used for measuring oxidative stress in live cells (Cheloni, Cosio & Slaveykova, 2014). The dye is weakly fluorescent inside cells in a reduced state but exhibits bright green photostable fluorescence upon oxidation by ROS and subsequent binding to DNA (Gemelli et al, 2014; Stodůlková et al, 2015). Modifications to the procedure recommended by the supplier were made as described in Material and Methods in order to detect ROS positive B. braunii colonies.…”

Section: Resultsmentioning

confidence: 99%

Stress responses of the oil-producing green microalgaBotryococcus brauniiRace B

Cornejo-Corona

Thapa

Browne

et al. 2016

PeerJ

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Plants react to biotic and abiotic stresses with a variety of responses including the production of reactive oxygen species (ROS), which may result in programmed cell death (PCD). The mechanisms underlying ROS production and PCD have not been well studied in microalgae. Here, we analyzed ROS accumulation, biomass accumulation, and hydrocarbon production in the colony-forming green microalga Botryococcus braunii in response to several stress inducers such as NaCl, NaHCO3, salicylic acid (SA), methyl jasmonate, and acetic acid. We also identified and cloned a single cDNA for the B. braunii ortholog of the Arabidopsis gene defender against cell death 1 (DAD1), a gene that is directly involved in PCD regulation. The function of B. braunii DAD1 was assessed by a complementation assay of the yeast knockout line of the DAD1 ortholog, oligosaccharyl transferase 2. Additionally, we found that DAD1 transcription was induced in response to SA at short times. These results suggest that B. braunii responds to stresses by mechanisms similar to those in land plants and other organisms.

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“…Quambalaria cyanescens has further been reported as a symbiont of plants. Recently, few studies have reported broad-spectrum antimicrobial activity for Q. cyanescens, including antibacterial and antifungal activities (Kuan et al 2015, Stodůlková et al 2015.…”

Section: Ecology and Future Prospectsmentioning

confidence: 99%

A noteworthy record of endophytic Quambalaria cyanescens from Punica granatum in Iran.

Vahedi-Darmiyan¹,

Jahani²,

Mirzaee³

et al. 2017

Czech Mycol.

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During an investigation into endophytic fungi associated with pomegranate in the South Khorasan Province of Iran, 2015-2016, five isolates were recovered with the morphological and molecular characteristics of Quambalaria cyanescens. The present study is the first fully documented report of Q. cyanescens from Iran, providing insight into its geographic distribution and host range. Our study is also the first report of occurrence of Q. cyanescens as an endophyte in a member of the Lythraceae family.

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Biologically Active Metabolites Produced by the Basidiomycete Quambalaria cyanescens

Cited by 21 publications

References 31 publications

Identification and Characterization of a Rare Fungus, Quambalaria cyanescens, Isolated from the Peritoneal Fluid of a Patient after Nocturnal Intermittent Peritoneal Dialysis

Identification and Characterization of a Rare Fungus, Quambalaria cyanescens, Isolated from the Peritoneal Fluid of a Patient after Nocturnal Intermittent Peritoneal Dialysis

Stress responses of the oil-producing green microalgaBotryococcus brauniiRace B

A noteworthy record of endophytic Quambalaria cyanescens from Punica granatum in Iran.

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