Biologically Active Compositions Containing Neurotrophic Factors Change the Contractile Activity of Detrusor of Rats with Infravesical Obstruction

Globa, Vyacheslav; Bondarenko, T. N.; Божок, Г. А.; Samburg, Y.; Legach, E. I.

doi:10.15407/cryo30.02.188

“…We investigated the contractility of the UB and the biochemical parameters of the blood of rats after IVO due to the use of BACs obtained from SG. In animals that were injected with a cryo extract of SG and conditioned medium obtained from a culture of cryopreserved MG, the most pronounced increase in body weight, the smallest indicators of UB weight and mass coefficient were found, and an increase in contractile activity and maximum amplitude of bladder contractions in response to various stimuli was noted [21,22]. The results of the current study were probably obtained by us due to the fact that NFs mediate hypertrophy of smooth muscle cells, hyperplasia of transitional cell epithelium and connective tissue [4,23].…”

Section: Discussionmentioning

confidence: 99%

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2020

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The morphological features of the urinary bladder (UB) after administration of biologically active compositions (BACs) containing neurotrophic factors (NFs) in female rats with infravesical obstruction (IVO) were studied. IVO was modeled by the ligature method. A cryo extract of the spinal ganglia (SG), conditioned media from cultures of native and cryopreserved mantle gliocytes (MG), and the drug Cortexin were used as the BACs. The BACs were injected intraperitoneally to the animals for 10 days. The morphometric parameters of the UB were determined by studying all its shells and their structure. The BACs obtained from the SG participated in the remodeling of the UB wall, which was changed during the IVO. Exogenous NFs insignificantly affected the hyperplasia of the transitional epithelium, stimulated regression and progression of the enlarged muscle layer of the detrusor, increased the proliferation of connective tissue, and increased the wall thickness of the UB. Proceeding from the above, NFs reduced the indices of the ratio of the muscle layer/wall of the UB, mediated by IVO.

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“…We investigated the contractility of the UB and the biochemical parameters of the blood of rats after IVO due to the use of BACs obtained from SG. In animals that were injected with a cryo extract of SG and conditioned medium obtained from a culture of cryopreserved MG, the most pronounced increase in body weight, the smallest indicators of UB weight and mass coefficient were found, and an increase in contractile activity and maximum amplitude of bladder contractions in response to various stimuli was noted [21,22]. The results of the current study were probably obtained by us due to the fact that NFs mediate hypertrophy of smooth muscle cells, hyperplasia of transitional cell epithelium and connective tissue [4,23].…”

Section: Discussionmentioning

confidence: 99%

Untitled

2020

JPP

0

View full text Add to dashboard Cite

The morphological features of the urinary bladder (UB) after administration of biologically active compositions (BACs) containing neurotrophic factors (NFs) in female rats with infravesical obstruction (IVO) were studied. IVO was modeled by the ligature method. A cryo extract of the spinal ganglia (SG), conditioned media from cultures of native and cryopreserved mantle gliocytes (MG), and the drug Cortexin were used as the BACs. The BACs were injected intraperitoneally to the animals for 10 days. The morphometric parameters of the UB were determined by studying all its shells and their structure. The BACs obtained from the SG participated in the remodeling of the UB wall, which was changed during the IVO. Exogenous NFs insignificantly affected the hyperplasia of the transitional epithelium, stimulated regression and progression of the enlarged muscle layer of the detrusor, increased the proliferation of connective tissue, and increased the wall thickness of the UB. Proceeding from the above, NFs reduced the indices of the ratio of the muscle layer/wall of the UB, mediated by IVO.

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“…Elbadawi et al (1993) виявили зв'язок між дегенеративними змінами аксонів у стінці СМ і порушенням скоротливої здатності детрузора у хворих на ДГПЗ [37]. V. Globa et al (2020) зазначили, що відновлення іннервації СМ після оперативного лікування ДГПЗ спостерігають не більше ніж у 80% хворих [52]. J.A.…”

Section: ремоделювання см у хворих на дгпзunclassified

Bladder Decompensation in Patients with Benign Prostatic Hyperplasia: Causes, Complications, Rehabilitation

Leonid¹,

Roman²

2021

HM

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Background Despite the high efficiency of surgical methods in correction of bladder outlet obstruction (BOO) caused by benign prostatic hyperplasia (BPH), a significant proportion of patients (up to 35%) remains after surgery with impaired contractile function of the bladder and lower urinary tract symptoms (LUTS). Both are consequences of structural and functional changes of the detrusor due to long-term exposure to obstructive factor. Today there is a lack of systematic reviews that provide a specialist with an integrative conception of pathological changes in the bladder wall caused by BPH associated prolonged BOO and evidence based methods of detrusor rehabilitation. The objective: systematization of modern conceptions about structural and functional changes in the bladder of patients with BPH caused by prolonged BOO, and methods of their correction. Materials and methods. An analytical review of the literature covering pathogenesis of bladder remodeling due to BPH associated long-term BOO and methods of bladder rehabilitation was performed. Literature sources were searched in PubMed, Google Scholar, Scopus and Web of Science databases by keywords. The search depth was 40 years. 74 relevant publications were selected for analysis. Results. Bladder remodeling due to obstructive factor is a complex staged process that involves all layers of the wall at the tissue, cellular and subcellular levels, affecting not only the executive structures (urothelium, smooth-muscle syncytium, fibrous connective tissue matrix), but also the system metabolism. Depending on the nature of the changes, there are 3 stages of this process: compensation, subcompensation and decompensation. In the compensatory stage, an increase of the load on the bladder leads to hypertrophy of smooth muscle fibers. At the same time, there is a reorganization of the vascular bed – neoangiogenesis, which should provide increased energy needs of muscles. In the stage of subcompensation, vicarious hypertrophy and neoangiogenesis cease. The most prominent disorders of the structure and function of the bladder are observed in the stage of decompensation. It includes a number of pathological processes: hypoxia, anaerobic metabolism, oxidative stress, inflammation, changes in the paracrine environment (increased levels of HIF-1α, vascular endothelial growth factor (VEGF) and angiopoietin-1). It is characterized by progressive loss of contractile function of the detrusor due to the death of smooth muscle cells and neurons, deterioration of viscoelastic characteristics of the bladder wall due to excessive collagen synthesis by fibroblasts, loss of barrier properties of the mucous membrane due to dystrophy. It was found that the severity of these pathological changes correlates with the severity of LUTS in patients who underwent surgery for BPH. The current arsenal of measures for bladder rehabilitation is quite diverse and includes periodic sterile catheterization, pharmacotherapy (cholinesterase inhibitors, antioxidants), physiotherapy (electrical stimulation, muscles training) and plastic surgery. However, there is still a lack of high-level studies to prove their effectiveness in patients who have undergone prostate surgery for BOO caused by BPH. Conclusions. Persistence of LUTS in patients who have undergone prostate surgery due to BOO may be caused by bladder decompensation. Long-term bladder decompensation due to prolonged exposure to obstructive factor is a complex process that includes decreased contractile activity, deterioration of the viscoelastic characteristics of the bladder, and impaired mucosal barrier function. Further research is needed to develop an effective bladder rehabilitation protocol.

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Biologically Active Compositions Containing Neurotrophic Factors Change the Contractile Activity of Detrusor of Rats with Infravesical Obstruction

Cited by 5 publications

References 33 publications

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Bladder Decompensation in Patients with Benign Prostatic Hyperplasia: Causes, Complications, Rehabilitation

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