Aim:
The study aimed to determine in vitro pharmacological effects of modified Ag nanoparticles
(AgNPs).
Background:
AgNPs are considered antimicrobial agents. However, the cytotoxicity of chemically synthesized
AgNPs (cAgNPs) has raised challenges that limit their use.
objective:
To examine the antimicrobial and cytotoxicity effects of AgNPs synthesized using Cirsium Congestum extract modified by chitosan/alginate (Ch/ALG-gAgNPs).
Objective:
The purpose of the study was to examine the antimicrobial and cytotoxicity effects of AgNPs synthesized
using Cirsium congestum extract modified by chitosan/alginate AgNPS (Ch/ALG-gAgNPs).
method:
Nanoparticles were characterized using TEM, DLS, XRD, and FTIR. Resistant strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used for the antimicrobial analysis of Ch/ALG-gAgNPs using disk diffusion and microdilution methods. The effects of NPs on cell viability and apoptosis in L929 normal cells were determined using MTT assay and annexin/PI staining, respectively.
Methods:
Nanoparticles were characterized using TEM, DLS, XRD, and FTIR. Resistant strains of
Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used for the antimicrobial analysis of
Ch/ALG-gAgNPs using disc diffusion and microdilution methods. The effects of NPs on cell viability and
apoptosis in L929 normal cells were determined using MTT assay and annexin/PI staining, respectively.
Results:
Physicochemical characterizations confirmed Ch/ALG-gAgNPs to be spherical and uniformly dispersed,
and their size ranged from 50 to 500 nm. Ch/ALG-gAgNPs inhibited the growth of microbial strains in
a dose-dependent manner. The antibacterial effect of Ch/ALG-gAgNPs was significantly higher than cAgNPs.
The Ch/ALG-gAgNPs showed little cytotoxicity against normal cells at concentrations less than 50 μg/ml. Cytotoxicity
effects of Ch/ALG-gAgNP were less than cAgNPs. Flow cytometry and real-time PCR results
showed a decrease in apoptosis percentage and BAX marker in the presence of Ch/ALG-gAgNPs relative to
when the cell was treated with cAgNPs.
Conclusion:
Current findings introduce novel gAgNPs modified with chitosan/alginate for use in medicine.