Biological synthesis of l-ascorbic acid in animal tissues: conversion of l-gulonolactone into l-ascorbic acid

Phenotypic drift in osmotic fragility of Sahel goat erythrocytes associated with variability of median fragility

“…Similarly, EOF of goats was increased after transport stress, but supplemental administration of ascorbic acid to the stressed goats reduced the effect on EOF (Minka & Ayo, 2010). Endogenous ascorbic acid synthesis occurs in the liver microsomes of goats (Chatterjee et al, 1960) and adaptation to stress in goats would seem to depend on their capacity to upregulate ascorbic acid biosynthesis to counter stressful situations, thereby mediating the fluctuations in the osmotic stability of erythrocytes. It would seem that stressadapted goats have decreased EOF.…”

Effects of vitamins A and D on the biosynthesis of l-ascorbic acid by rat-liver microsomes

“…L-Gulonolactone was prepared as described by Chatterjee et al (1960a). D-Glucuronolactone was purchased from California Corp. for Biochemical Research (Los Angeles, Calif., U.S.A.).…”

“…Rats were killed by decapitation and microsomes were prepared from the livers by the method of Chatterjee et al (1960a The experiments were performed in the presence of pyrophosphate, pH7-4 (20mm), since in its absence there is a diminished formation of L-ascorbic acid from L-gulonolactone by rat-liver microsomes (Chatterjee, Kar, Ghosh & Guha, 1961). Ascorbic acid and protein in the microsomal preparations were estimated as described by Chatterjee et al (1960a RESULTS Effects of hypervitaminosis A and hypervitaminosis D on the conversion of D-glucuronolactone and Lgulonolactone into L-ascorbic acid by rat-liver microsomes. Table 1 shows that in hypervitaminosis A the microsomal synthesis of L-ascorbic acid from both D-glucuronolactone and L-gulonolactone is decreased when compared with the controls.…”

Inhibition of Insulin Secretion and Increase of Plasma Non-Esterified Fatty Acids Induced by Biotin Deficiency in Osteogenic Disorder Shionogi Rats.

“…Dakshinamurti and Mistry [3] demonstrated that in biotin-deficient rats induced by feeding of a diet containing raw egg white, the liver and adrenals contained less than half the total ascorbic acid of the control rats. Ascorbic acid is synthesized from D-glucose via the D-glucuronic acid pathway [4,5]. They indicated that the reduction in ascorbic acid content was produced by decreased activity of L-gulonolactone oxidase activity in the L-ascorbic acid synthesis system [3].…”