Biological synthesis of copper oxide nanoparticles using marine endophytic actinomycetes and evaluation of biofilm producing bacteria and A549 lung cancer cells

Zhao, Hongxia; Maruthupandy, Muthuchamy; Galil, Fahd Mohammed Abd Al; Chackaravarthi, Gnanasekaran; Ramachandran, Govindan; Chelliah, Chenthis Kanisha

doi:10.1016/j.jksus.2022.101866

Cited by 35 publications

(21 citation statements)

References 42 publications

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“…VLC. induced toxic effects on human lung and esophageal carcinoma cells in a dose-dependent manner, with IC 50 values of 500 and 37.52 μg/ml, respectively ( Nakhaeepour et al, 2019 ; Zhao et al, 2022 ). In addition, low toxicity of CuONPs on normal HDF cells in the present study is in line with another research, which reported minimal toxic effects of CuONPs on human dermal fibroblasts ( Sulaiman et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Bacterial-mediated synthesis and characterization of copper oxide nanoparticles with antibacterial, antioxidant, and anticancer potentials

Talebian

Shahnavaz²,

Nejabat³

et al. 2023

Front. Bioeng. Biotechnol.

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The application of novel bacterial strains for effective biosynthesis of nanoparticles minimizes negative environmental impact and eliminates challenges of available approaches. In the present study, cell-free extract of Stenotrophomonas sp. BS95. was used for synthesis of copper oxide nanoparticles (CuONPs). Characterization of crude and calcined CuONPs was carried out by UV-vis spectroscopy, X-ray diffraction (XRD), fourier transform infrared (FTIR) spectroscopy, zeta potential, dynamic light scattering, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. Afterward, biogenic CuONPs were evaluated for antibacterial, antioxidant, and cytotoxic effects using broth micro-dilution method, DPPH assay and alamarBlue assay, respectively. Finally, molecular mechanisms behind anticancer effects of CuONPs was ascertained by real time PCR. UV-vis absorbance spectra registered surface plasmon resonance peaks at 286 nm and 420 nm for crude and calcined CuONPs, respectively. FTIR spectra exhibited bands associated with organic functional groups of bacterial proteins, confirming capping and functionalization of CuONPs. The average crystallite size of crude and calcined CuONPs was determined as 18.24 and 21.3 nm by XRD, respectively. The average zeta potentials of crude and calcined CuONPs were as −28.57 ± 5.13 and −29.47 ± 4.78 mV, respectively, indicating their high stability. Electron microscopy revealed that crude and calcined CuONPs were roughly spherical particles with an average size of 35.24 ± 4.64 and 43.68 ± 2.31 nm, respectively. Biogenic CuONPs induced antibacterial effects with minimal inhibitory concentrations ranging from 62.5 to 1,000 μg/ml against Gram-negative and Gram-positive strains. The antioxidant activity of crude and calcined CuONPs was found to be 83% ± 2.64% and 78% ± 1.73%, respectively. More intriguingly, CuONPs exerted considerable cytotoxic effects on human colon and gastric adenocarcinoma cells, while induced low toxicity on normal cells. Anticancer effects of biogenic CuONPs were confirmed by significant changes induced in the expression of apoptosis-related genes, including P53, BAX, BCL2 and CCND1. Hence, biosynthesized CuONPs could be considered as potential antimicrobial, antioxidant and anticancer agents.

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Section: Discussionmentioning

confidence: 99%

Bacterial-mediated synthesis and characterization of copper oxide nanoparticles with antibacterial, antioxidant, and anticancer potentials

Talebian

Shahnavaz²,

Nejabat³

et al. 2023

Front. Bioeng. Biotechnol.

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“…Additionally, the research showed that the nitrate reductase enzyme was dependent on the NADH molecule and reduced Ag ions to create stable Ag nanoparticles [ 66 ]. In recent research, CuO nanoparticles were synthesized using marine endophytic actinomycetes CKV1 as the stabilizing and reducing agent [ 67 ]. These actinomycete-mediated CuO nanoparticles had a spherical morphology with an average size of 20 nm, as revealed from TEM analysis, and exerted activity against bacteria and cancer.…”

Section: Green Synthesis Of Nanomaterialsmentioning

confidence: 99%

Plant and Microbial Approaches as Green Methods for the Synthesis of Nanomaterials: Synthesis, Applications, and Future Perspectives

et al. 2023

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The unique biological and physicochemical characteristics of biogenic (green-synthesized) nanomaterials (NMs) have attracted significant interest in different fields, with applications in the agrochemical, food, medication delivery, cosmetics, cellular imaging, and biomedical industries. To synthesize biogenic nanomaterials, green synthesis techniques use microorganisms, plant extracts, or proteins as bio-capping and bio-reducing agents and their role as bio-nanofactories for material synthesis at the nanoscale size. Green chemistry is environmentally benign, biocompatible, nontoxic, and economically effective. By taking into account the findings from recent investigations, we shed light on the most recent developments in the green synthesis of nanomaterials using different types of microbes and plants. Additionally, we cover different applications of green-synthesized nanomaterials in the food and textile industries, water treatment, and biomedical applications. Furthermore, we discuss the future perspectives of the green synthesis of nanomaterials to advance their production and applications.

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“…UV-visible absorption spectroscopy can be used to characterize metallic nanoparticles based on surface plasmon resonance (SPR) (Upadhyay et al, 2023). UV-visible spectroscopy (wavelength scan Frontiers in Chemistry frontiersin.org between 200 and 1,000 nm) was used to observe the solution resulting from the bluish-to-greenish color alterations of copper-tolerant bacteria (Zhao et al, 2022). The spectra of CuO-NPs generated employing coppertolerant bacteria showed pronounced absorption at 285 nm wavelength, confirming the conversion of the starting material (copper sulfate) to the final product (CuO nanoparticles) as shown in Figure 3.…”

Section: Influence Of Cuo-nps On Maize Seedlingmentioning

confidence: 99%

Bacteria assisted green synthesis of copper oxide nanoparticles and their potential applications as antimicrobial agents and plant growth stimulants

et al. 2023

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Copper oxide nanoparticles (CuO-NPs) have piqued the interest of agricultural researchers due to their potential application as fungicides, insecticides, and fertilizers. The Serratia sp. ZTB29 strain, which has the NCBI accession number MK773873, was a novel isolate used in this investigation that produced CuO-NPs. This strain can survive concentrations of copper as high as 22.5 mM and can also remove copper by synthesizing pure CuO-NPs. UV-VIS spectroscopy, DLS, Zeta potential, FTIR, TEM, and XRD techniques were used to investigate the pure form of CuO-NPs. The synthesized CuO-NPs were crystalline in nature (average size of 22 nm) with a monoclinic phase according to the XRD pattern. CuO-NPs were found to be polydisperse, spherical, and agglomeration-free. According to TEM and DLS inspection, they ranged in size from 20 to 40 nm, with a typical particle size of 28 nm. CuO-NPs were extremely stable, as demonstrated by their zeta potential of −15.4 mV. The ester (C=O), carboxyl (C=O), amine (NH), thiol (S-H), hydroxyl (OH), alkyne (C-H), and aromatic amine (C-N) groups from bacterial secretion were primarily responsible for reduction and stabilization of CuO-NPs revealed in an FTIR analysis. CuO-NPs at concentrations of 50 μg mL−1 and 200 μg mL−1 displayed antibacterial and antifungal activity against the plant pathogenic bacteria Xanthomonas sp. and pathogenic fungus Alternaria sp., respectively. The results of this investigation support the claims that CuO-NPs can be used as an efficient antimicrobial agent and nano-fertilizer, since, compared to the control and higher concentrations of CuO-NPs (100 mg L−1) considerably improved the growth characteristics of maize plants.

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Biological synthesis of copper oxide nanoparticles using marine endophytic actinomycetes and evaluation of biofilm producing bacteria and A549 lung cancer cells

Cited by 35 publications

References 42 publications

Bacterial-mediated synthesis and characterization of copper oxide nanoparticles with antibacterial, antioxidant, and anticancer potentials

Bacterial-mediated synthesis and characterization of copper oxide nanoparticles with antibacterial, antioxidant, and anticancer potentials

Plant and Microbial Approaches as Green Methods for the Synthesis of Nanomaterials: Synthesis, Applications, and Future Perspectives

Bacteria assisted green synthesis of copper oxide nanoparticles and their potential applications as antimicrobial agents and plant growth stimulants

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