Biological Synthesis and Characterization of Copper Oxide Nanoparticles Using Antigonon Leptopus Leaf Extract and Their Antibacterial Activity

Sravanthi, M; Kumar, Dharmendra; Уша, Б. В.; Rao, M.Mahendra; Hemalatha, K.; Ravichandra, M.

doi:10.21474/ijar01/1251

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…The copper nanoparticles formation is usually con rmed by UV-Vis spectroscopy studies of colored solution [18]. The concept of obtaining the peak in the UV-Vis spectrum is that, on the surface of metal nanoparticles the free electrons will be present which undergoes strong repulsion with the light of speci c wavelength [19].…”

Section: Ultraviolet Visible Spectrophotometermentioning

confidence: 99%

Fabrication and Characterization of Copper Nanoparticles by Green Synthesis Approach Using Plectranthus Amboinicus Leaves Extract

Parthasarathy

Jayacumar

Chakraborty

et al. 2020

Preprint

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The field of nanotechnology is gaining interest among the researchers towards the eco-friendly way of synthesis of nanoparticles. In this project, green synthesis technique was employed to induce the synthesis of copper nanoparticles using Plectranthus amboinicus, i.e. Mexican mint, identified as Coleus amboinicus leaf extract. We report an eco-friendly synthesis of copper nanoparticle using Plectranthus amboinicus leaf extract, which is a simple and an ostentatiously rapid method which produces stable nanoparticles. The copper sulphate solution was naturally employed as a precursor for synthesizing the copper nanoparticles. The extract of the plant Plectranthus amboinicus was found to showcase excellent reducing and stabilizing properties. By using Ultraviolet-Visible spectroscopy, Zeta Potential, and X-Ray Diffraction (XRD) studies, it was confirmed that copper nanoparticles have been synthesized. The UV-Spectrometer analysis shows the characteristic peak indicating the synthesis of copper nanoparticles. The pattern of XRD analysis showed particle size of 16 - 25 nm and it reveals high crystallinity of the copper nanoparticles. Zeta potential was done to find the charge of the nanoparticles and size distribution which showed to have significant stability. This method proves to be cost-effective, can be performed at ease, and it’s also free of pollutants.

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Section: Ultraviolet Visible Spectrophotometermentioning

confidence: 99%

Fabrication and Characterization of Copper Nanoparticles by Green Synthesis Approach Using Plectranthus Amboinicus Leaves Extract

Parthasarathy

Jayacumar

Chakraborty

et al. 2020

Preprint

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“…An antibacterial assay using a well-cut method was used to determine the MIC of biosynthesized and chemically synthesized CuO NPs against two test pathogens- Staphylococcus aureus ( S. aureus ) and Pseudomonas aeruginosa ( P. aeruginosa ). 88 The algal biosynthesized CuO NPs were denoted as CuO-1, CuO-2, and CuO-3 and chemically synthesized CuO NPs were denoted as CuO-4. The MIC values for both test pathogens, S. aureus and P. aeruginosa at various CuO NPs concentrations were shown in Tables 9 and 10 , respectively.…”

Section: Resultsmentioning

confidence: 99%

An Eco-Friendly Synthesis Approach for Enhanced Photocatalytic and Antibacterial Properties of Copper Oxide Nanoparticles Using Coelastrella terrestris Algal Extract

Khandelwal,

Choudhary,

Harish

et al. 2024

IJN

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Background In the current scenario, the synthesis of nanoparticles (NPs) using environmentally benign methods has gained significant attention due to their facile processes, cost-effectiveness, and eco-friendly nature. Methods In the present study, copper oxide nanoparticles (CuO NPs) were synthesized using aqueous extract of Coelastrella terrestris algae as a reducing, stabilizing, and capping agent. The synthesized CuO NPs were characterized by X-ray diffraction (XRD), UV-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and field emission scanning electron microscopy (FE-SEM) coupled with energy-dispersive X-ray spectroscopy (EDS). Results XRD investigation revealed that the biosynthesized CuO NPs were nanocrystalline with high-phase purity and size in the range of 4.26 nm to 28.51 nm. FTIR spectra confirmed the existence of secondary metabolites on the surface of the synthesized CuO NPs, with characteristic Cu–O vibrations being identified around 600 cm −1 , 496 cm −1 , and 440 cm −1 . The FE-SEM images predicted that the enhancement of the algal extract amount converted the flattened rice-like structures of CuO NPs into flower petal-like structures. Furthermore, the degradation ability of biosynthesized CuO NPs was investigated against Amido black 10B (AB10B) dye. The results displayed that the optimal degradation efficacy of AB10B dye was 94.19%, obtained at 6 pH, 50 ppm concentration of dye, and 0.05 g dosage of CuO NPs in 90 min with a pseudo-first-order rate constant of 0.0296 min −1 . The CuO-1 NPs synthesized through algae exhibited notable antibacterial efficacy against S. aureus with a zone of inhibition (ZOI) of 22 mm and against P. aeruginosa with a ZOI of 17 mm. Conclusion Based on the findings of this study, it can be concluded that utilizing Coelastrella terrestris algae for the synthesis of CuO NPs presents a promising solution for addressing environmental contamination.

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“…Standard deviation (S.D): 0.153 Cut-Off: 0.567 ‫٭‬moderate biofilm producer ‫٭٭‬strong biofilm producer Accordingly, isolates were classified as follows: O.D < 0.12 = no biofilm producer or weak biofilm producer, 0.12 < O.D ≤ 0.24 = moderate biofilm producer and 0.24 < O.D = strong biofilm producer (Magesh et al, 2013) [27]. The effect of nanometals on inhibition of the biofilm formation is due to released that metal ions subsequently may bind with DNA molecules and lead to disordering of the helical structure by cross-linking within and between the nucleic acid strands and also disrupt the biochemical processes and protein denaturation and cause cell death [28,29]. Another proposed mechanism is, there will be metal ions released from the nanoparticles that may attach to the negatively charged bacterial cell wall and rupture it, thereby leading to protein denaturation and cause cell death [28].…”

Section: The Effect Of Ag-sio 2 Nps On Biofilm Formation Using Microtiter Plate Methodsmentioning

confidence: 99%

Ag-SiO2 nanoparticales prepared by Sol-Gel methods for antibacterial effect

Mohammed¹

2019

IJP

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Sol-gel method was use to prepare Ag-SiO2 nanoparticles. Crystal structure of the nanocomposite was investigated by means of X-ray diffraction patterns while the color intensity was evaluated by spectrophotometry. The morphology analysis using atomic force microscopy showed that the average grain sizes were in range (68.96-75.81 nm) for all samples. The characterization of Ag-SiO2 nanoparticles were investigated by using Scanning Electron Microscopy (SEM). Ag-SiO2 NPs are highly stable and have significant effect on both Gram positive and negative bacteria. Antibacterial properties of the nanocomposite were tested with the use of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria. The results have shown antibacterial effect of the Ag-SiO2 prepared as nanogel and nanopowder states, while the Ag-SiO2 nanopowder showed the highest capability against S. aureus. Both methods of biofilm showed an inhibition effect for Ag-SiO2 NPs, the synthetic Ag-SiO2 NPs showed highest inhibition effect on Gram positive bacteria S. aureus by using the biofilm microtiter method.

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Biological Synthesis and Characterization of Copper Oxide Nanoparticles Using Antigonon Leptopus Leaf Extract and Their Antibacterial Activity

Cited by 7 publications

References 12 publications

Fabrication and Characterization of Copper Nanoparticles by Green Synthesis Approach Using Plectranthus Amboinicus Leaves Extract

Fabrication and Characterization of Copper Nanoparticles by Green Synthesis Approach Using Plectranthus Amboinicus Leaves Extract

An Eco-Friendly Synthesis Approach for Enhanced Photocatalytic and Antibacterial Properties of Copper Oxide Nanoparticles Using Coelastrella terrestris Algal Extract

Ag-SiO2 nanoparticales prepared by Sol-Gel methods for antibacterial effect

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