Biological stability of a strain of Cowpea severe mosaic virus over 20 years

Lima, J.A.; Nascimento, Aline Kelly Queiroz do; Silva, Ana Kelly Firmino da; Aragão, Maria do Livramento

doi:10.1590/s1806-66902012000100013

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…Another common characteristic of this compatible interaction was the presence of symptoms in young tertiary leaves, showing the rapid accumulation and systemic virus movement in the course of infection (Figure B). These symptoms were similar to those found in other cowpea CPSMV-susceptible cultivars. , …”

Section: Resultssupporting

confidence: 87%

“…These symptoms were similar to those found in other cowpea CPSMV-susceptible cultivars. 4,16 Phenolic, H 2 O 2 , and Lignin Contents in Cowpea Associated with the CPSMV Disease Development…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Although well adapted to unfavorable environmental conditions, pest and pathogen attacks limit cowpea production . Cowpea can be infected by more than 20 different virus species and Cowpea Severe Mosaic Virus (CPSMV) is one of the most devastating, responsible for huge losses in productivity. , CPSMV is classified into the order picornavirales , family Secoviridae , subfamily Comovirinae, and the genus Comovirus. Viruses that belong to Comovirus are nonenveloped, have capsids with icosahedral shapes, a bipartite linear single-stranded positive RNA (ssRNA+) genome composed of RNA-1 (6–8 Kb) and RNA-2 (4 to 7 kb), which are translated into two polyproteins further processed in functional proteins. , …”

Section: Introductionmentioning

confidence: 99%

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Label-free Proteomic Reveals that Cowpea Severe Mosaic Virus Transiently Suppresses the Host Leaf Protein Accumulation During the Compatible Interaction with Cowpea (Vigna unguiculata [L.] Walp.)

Paiva¹,

Oliveira²,

Souza³

et al. 2016

J. Proteome Res.

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Viruses are important plant pathogens that threaten diverse crops worldwide. Diseases caused by Cowpea severe mosaic virus (CPSMV) have drawn attention because of the serious damages they cause to economically important crops including cowpea. This work was undertaken to quantify and identify the responsive proteins of a susceptible cowpea genotype infected with CPSMV, in comparison with mock-inoculated controls, using label-free quantitative proteomics and databanks, aiming at providing insights on the molecular basis of this compatible interaction. Cowpea leaves were mock- or CPSMV-inoculated and 2 and 6 days later proteins were extracted and analyzed. More than 3000 proteins were identified (data available via ProteomeXchange, identifier PXD005025) and 75 and 55 of them differentially accumulated in response to CPSMV, at 2 and 6 DAI, respectively. At 2 DAI, 76% of the proteins decreased in amount and 24% increased. However, at 6 DAI, 100% of the identified proteins increased. Thus, CPSMV transiently suppresses the synthesis of proteins involved particularly in the redox homeostasis, protein synthesis, defense, stress, RNA/DNA metabolism, signaling, and other functions, allowing viral invasion and spread in cowpea tissues.

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Section: Resultssupporting

confidence: 87%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Label-free Proteomic Reveals that Cowpea Severe Mosaic Virus Transiently Suppresses the Host Leaf Protein Accumulation During the Compatible Interaction with Cowpea (Vigna unguiculata [L.] Walp.)

Paiva¹,

Oliveira²,

Souza³

et al. 2016

J. Proteome Res.

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“…Surprisingly, two of the seven types of produced peptides were fragments of the cowpea severe mosaic virus (CPSMV) polyprotein RNA1. CPSMV is most commonly found in cowpea, but Lima et al ( 41 ) reported that the virus was also isolated from jack bean. All produced peptides were tested for toxicity prediction using a toxin prediction program ( 42 ), which revealed that all peptides (JF72) absorbed by the small intestine were non-toxic.…”

Section: Resultsmentioning

confidence: 99%

Jack Bean (Canavalia ensiformis) Tempeh: ACE-Inhibitory Peptide Formation during Absorption in the Small Intestine

Puspitojati

Cahyanto

Marsono

et al. 2023

Food Technol. Biotechnol. (Online)

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Research background. High blood pressure is the most significant cause of mortality globally. Some fermented foods include ACE inhibitor peptides that help fight this disease. The ability of fermented jack bean (tempeh) to inhibit ACE during consumption has not been demonstrated. This study identified and characterised ACE inhibitor peptides from jack bean tempehh produced by small intestine absorption using the everted intestinal sacs model. Experimental approach. Sequentially, the protein extract of jack bean tempehh and unfermented jack bean was hydrolysed using pepsin-pancreatin for 240 min. The hydrolysed samples were then evaluated for the peptide absorption using three-segmented everted intestinal sacs (duodenum, jejunum, and ileum). The peptides absorbed from all intestine segments were mixed as the mixtures of peptides absorbed in the small intestine. Results and conclusion. The data showed that both jack bean tempehh and unfermented jack bean possessed the same peptide absorption pattern, with the highest percentage of peptide absorption in the jejunum, followed by the duodenum and ileum. The absorbed peptides of jack bean tempeh exhibited the same strong ACE inhibition activity in all intestine segments, while the unfermented jack bean showed strong activity only in the jejunum. The mixture of peptides that absorbed in the small intestine from jack bean tempeh possessed higher ACE inhibitory activity (81.09 %) than the unfermented jack bean (72.22 %). The peptides produced from jack bean tempeh were identified as pro-drug ACE inhibitors and possessed the mixed inhibition pattern. The mixture peptides consisted of seven types of the peptide with a molecular weight of 826,86 – 978,20 Da (DLGKAPIN, GKGRFVYG, PFMRWR, DKDHAEI, LAHLYEPS, KIKHPEVK and LLRDTCK). Novelty and scientific contribution. This study discovered that consuming jack bean tempeh generated more potent ACE inhibitory peptides during small intestine absorption than boiling jack beans. Absorbed tempeh peptides provide seven peptides with high ACE inhibitory action.

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A resistant cowpea (Vigna unguiculata [L.] Walp.) genotype became susceptible to cowpea severe mosaic virus (CPSMV) after exposure to salt stress

Varela

Oliveira

Komatsu

et al. 2019

Journal of Proteomics

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Biological stability of a strain of Cowpea severe mosaic virus over 20 years

Cited by 4 publications

References 11 publications

Label-free Proteomic Reveals that Cowpea Severe Mosaic Virus Transiently Suppresses the Host Leaf Protein Accumulation During the Compatible Interaction with Cowpea (Vigna unguiculata [L.] Walp.)

Label-free Proteomic Reveals that Cowpea Severe Mosaic Virus Transiently Suppresses the Host Leaf Protein Accumulation During the Compatible Interaction with Cowpea (Vigna unguiculata [L.] Walp.)

Jack Bean (Canavalia ensiformis) Tempeh: ACE-Inhibitory Peptide Formation during Absorption in the Small Intestine

A resistant cowpea (Vigna unguiculata [L.] Walp.) genotype became susceptible to cowpea severe mosaic virus (CPSMV) after exposure to salt stress

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