Biological Reaction to Small-Diameter Vascular Grafts Made of Silk Fibroin Implanted in the Abdominal Aortae of Rats

Fukayama, Toshiharu; Takagi, Kenta; Tanaka, Ryou; Hatakeyama, Yui; Aytemiz, Derya; Suzuki, Yuichi; Asakura, Tetsuo

doi:10.1016/j.avsg.2014.10.008

Cited by 38 publications

(43 citation statements)

References 33 publications

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“…Each resulting tube was coated with an SF solution, according to previously described procedures. 22 , 23 , 25 , 26 ) Each tube was subsequently dried, fixed in ethanol, and removed from the central core to yield a graft. Light microscopy and scanning electron microscopy (SEM; VE-7800, Keyence, Tokyo, Japan) were used to assess the internal and external morphologies of the grafts pre-implantation.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have described the benefits of SF vascular grafts in small rats. 21 – 23 ) However, the long-term patency rates and histological effects of these grafts have not been clarified in larger animal models. It is key to test the SF grafts in larger animals, as the biological responses after bypass surgery with synthetic grafts, including patency, flow, coagulation, and conduit healing, vary among species.…”

Section: Introductionmentioning

confidence: 99%

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Histological Reactions and the In Vivo Patency Rates of Small Silk Vascular Grafts in a Canine Model

Haga

Yamamoto

Okamoto

et al. 2017

Annals of Vascular Diseases

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Objective: To evaluate in vivo patency rates of silk fibroin (SF) vascular grafts and resulting histological reactions in a canine model.Methods: To generate 3.5-mm inner diameter vessels, a combination of plaited silk fibers were wound with cocoon filaments and subsequently coated with an SF solution. The resulting SF grafts (n=35) were implanted into the carotid arteries of male beagles (age, 1–2 years; body weight: 9.0–10.5 kg). Expanded polytetrafluoroethylene (4-mm inner diameter, ePTFE) grafts (n=5) were used as controls. Graft patency was monitored via ultrasonography with histological changes analyzed via microscopic examination.Results: Compared with animals that received the ePTFE grafts, animals that received SF grafts exhibited the same thickness of luminal layers and fibrin accumulation and collagen fiber replacement with endothelialization at 3 months post-implantation via histological examination. The patency rates of the SF and the ePTFE grafts at 6 months post-implantation were 7.8% and 0%, respectively.Conclusion: This canine model study demonstrated that SF grafts induce unique histological reactions but fail to achieve long-term patency.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histological Reactions and the In Vivo Patency Rates of Small Silk Vascular Grafts in a Canine Model

Haga

Yamamoto

Okamoto

et al. 2017

Annals of Vascular Diseases

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“…18 Furthermore, formation of endothelial cells and smooth muscle cells were found in the earlystages. Although previous studies show that SF is useful for knitted tube material, 19,20 numerous problems still exist. The knitted SF tube cannot maintain its anastomotic strength.…”

Section: Introductionmentioning

confidence: 99%

Effect of fibroin sponge coating on in vivo performance of knitted silk small diameter vascular grafts

et al. 2015

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ABSTRACT. Vascular grafts under 5 mm or less in diameter are not developed due to a problem caused by early thrombus formation, neointimal hyperplasia, etc. Bombyx mori silk fibroin (SF) which has biodegradability and tissue infiltration is focused as tube and coating material of vascular grafts. Coating is an important factor to maintain the strength of the anastomotic region of vascular grafts, and to prevent the blood leak from the vascular grafts after implantation. Therefore, in this research, we focused on the SF concentration of the coating solution, and tissue infiltration and remodeling were compared among each SF concentration. Silk poly (-ethylene) glycol diglycidyl ether (PGDE) coating with concentrations of 1.0%, 2.5%, 5.0%, and 7.5% SF were applied for the double-raschel knitted small-sized vessel with 1.5 mm diameter and 1cm in length. The grafts were implanted in the rat abdominal aorta and removed after 3 weeks or 3 months. Vascular grafts patency was monitored by ultrasound, and morphological evaluation was performed by histopathological examination. SF concentration had no significant effects on the patency rate. However, tissue infiltration was significantly higher in the sample of 2.5% SF in 3 weeks, and 1.0% and 2.5% SF in 3 months. Also, in comparison of length inside of the graft, stenosis were not found in 3 weeks, however, found with 5.0% and 7.5% in 3 months. From these results, it is clear that 2.5% SF coating is the most suitable concentration, based on the characteristics of less stenosis, early tissue infiltration, and less neointimal hyperplasia.

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“…Silk derived from silkworms was used in various tissue engineering fields as a natural biopolymer scaffold [26,36] owing to its excellent biological properties, such as biocompatibility, biodegradability, and tissue affinity [37]. Other reasons for choosing to employ silk as a vascular tissue engineering scaffolding biomaterial are its low thrombotic and immunologic properties and ease of clinical handling [37,38]. In consideration of these properties, silk was processed into various forms of vascular scaffolds, such as tubes, films, and patches [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Angioplasty Using 4-Hexylresorcinol-Incorporated Silk Vascular Patch in Rat Carotid Defect Model

Kim

et al. 2018

Applied Sciences

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The aim of this study was to evaluate and compare the efficacy of 4-hexylresorcinol (4-HR)-incorporated silk as a vascular patch scaffold to that of the commercial polytetrafluoroethylene (PTFE) vascular patch (GORE® ACUSEAL). The expression of the vascular endothelial cell growth factor-A (VEGF-A) after application of 4-HR was studied in RAW264.7 and HUVEC cells. In the animal study, a carotid artery defect was modeled in Sprague Dawley rats (n = 30). The defect was directly closed in the control group (n = 10), or repaired with the PTFE or 4-HR silk patch in the experimental groups (n = 10 per group). Following patch angioplasty, angiography was performed and the peak systolic velocity (PSV) was measured to evaluate the artery patency. The application of 4-HR was shown to increase the expression of VEGF-A in RAW264.7 and HUVEC cells. The successful artery patency rate was 80% for the 4-HR silk group, 30% for the PTFE group, and 60% for the control group. The PSV of the 4-HR silk group was significantly different from that of the control group at one week and three weeks post-angioplasty (p = 0.005 and 0.024). Histological examination revealed new regeneration of the arterial wall, and that the arterial diameter was well maintained in the 4-HR silk group in the absence of an immune reaction. In contrast, an overgrowth of endothelium was observed in the PTFE group. In this study, the 4-HR silk patch was successfully used as a vascular patch, and achieved a higher vessel patency rate and lower PSV than the PTFE patch.

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Biological Reaction to Small-Diameter Vascular Grafts Made of Silk Fibroin Implanted in the Abdominal Aortae of Rats

Cited by 38 publications

References 33 publications

Histological Reactions and the In Vivo Patency Rates of Small Silk Vascular Grafts in a Canine Model

Histological Reactions and the In Vivo Patency Rates of Small Silk Vascular Grafts in a Canine Model

Effect of fibroin sponge coating on in vivo performance of knitted silk small diameter vascular grafts

Angioplasty Using 4-Hexylresorcinol-Incorporated Silk Vascular Patch in Rat Carotid Defect Model

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