Biological Properties and Characterization of ASL50 Protein from Aged Allium sativum Bulbs

Abstract

Allium sativum is well known for its medicinal properties. The A. sativum lectin 50 (ASL50, 50 kDa) was isolated from aged A. sativum bulbs and purified by gel filtration chromatography on Sephacryl S-200 column. Agar well diffusion assay were used to evaluate the antimicrobial activity of ASL50 against Candida species and bacteria then minimal inhibitory concentration (MIC) was determined. The lipid A binding to ASL50 was determined by surface plasmon resonance (SPR) technology with varying concentrations. Electron microscopic studies were done to see the mode of action of ASL50 on microbes. It exerted antimicrobial activity against clinical Candida isolates with a MIC of 10-40 μg/ml and clinical Pseudomonas aeruginosa isolates with a MIC of 10-80 μg/ml. The electron microscopic study illustrates that it disrupts the cell membrane of the bacteria and cell wall of fungi. It exhibited antiproliferative activity on oral carcinoma KB cells with an IC50 of 36 μg/ml after treatment for 48 h and induces the apoptosis of cancer cells by inducing 2.5-fold higher caspase enzyme activity than untreated cells. However, it has no cytotoxic effects towards HEK 293 cells as well as human erythrocytes even at higher concentration of ASL50. Biological properties of ASL50 may have its therapeutic significance in aiding infection and cancer treatments.

Aged garlic and cancer: A systematic review

“…ASL50 exhibited antiproliferative activity on oral carcinoma KB cells with an IC50 of 36 μg/ml after treatment for 48 h and induced the apoptosis of cancer cells by inducing 2.5-fold higher caspase enzyme activity, a family of protease enzymes playing essential roles in programmed cell death, than untreated cells. [ 37 ]…”

“…[ 20 22 23 24 ] Furthermore, most animal and laboratory evidence reported effectiveness of aged garlic and components derived from aged garlic on tumor markers reduction. [ 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 ] However, some of them showed contradictory effects.…”

In-vitro Analysis of Chenopodium murale extract for resistance against potato virus Y

“…Lectins are known to induce cytotoxicity in cancer cells by interacting with them or their receptors to produce necrosis, autophagy, apoptosis or inhibit cell growth [9]. The cytotoxicity of lectin protein named ASL 50, isolated from Allium sativum, showed no cytotoxic effect on HEK cells and human erythrocytes, but on oral carcinoma KB cells showed the cytotoxic effect and IC50 was observed as 36 µg/ml [20]. Different cell lines were used to estimate the inhibitory concentration of Galanthus nivalis agglutinin (GNA).…”