Biological Preparation of Chitosan-Loaded Silver Nanoparticles: Study of Methylene Blue Adsorption as Well as Antibacterial Properties under Light

Abstract

Human beings have made significant progress in the medical field since antibiotics were widely used. However, the consequences caused by antibiotics abuse have gradually shown their negative effects. Antibacterial photodynamic therapy (aPDT) has the ability to resist drug-resistant bacteria without antibiotics, and as it is increasingly recognized that nanoparticles can effectively solve the deficiency problem of singlet oxygen produced by photosensitizers, the application performance and scope of aPDT are gradually being expanded. In this study, we used a biological template method to reduce Ag+ to silver atoms in situ with bovine serum albumin (BSA) rich in various functional groups in a 50 °C water bath. The aggregation of nanomaterials was inhibited by the protein’s multistage structure so that the formed nanomaterials have good dispersion and stability. It is unexpected that we used chitosan microspheres (CMs) loaded with silver nanoparticles (AgNPs) to adsorb methylene blue (MB), which is both a pollutant and photosensitive substance. The Langmuir adsorption isothermal curve was used to fit the adsorption capacity. The exceptional multi-bond angle chelating forceps of chitosan make it have a powerful physical adsorption capacity, and dehydrogenated functional groups of proteins with negative charge can also bond to positively charged MB to form a certain amount of ionic bonds. Compared with single bacteriostatic materials, the bacteriostatic capacity of the composite materials adsorbing MB under light was significantly improved. This composite material not only has a strong inhibitory effect on Gram-negative bacteria but also has a good inhibitory effect on the growth of Gram-positive bacteria poorly affected by conventional bacteriostatic agents. In conclusion, the CMs loaded with MB and AgNPs have some possible applications in the purification or treatment of wastewater in the future.

Hydrogels Based on Chitosan and Nanoparticles and Their Suitability for Dyes Adsorption from Aqueous Media: Assessment of the Last-Decade Progresses

“…Ren et al [ 39 ] prepared chitosan microspheres loaded with silver nanoparticles and used them to adsorb Methylene blue from water.…”

The multi-integrated DNA Bioinspired hydrogel with synergic effect of MB and AuNP with conductive, self-healing, and antibacterial properties as a wearable skin-like sensor

“…Ti 3 C 2 T x MXene nanosheets were synthesized via a modified hydrofluoric acid (HF) etching protocol (19). In brief, 2.00 g of Ti 3 AlC 2 powder and 2.00 g LiF were mixed in 20 mL of HCl solution (9 M).…”

“…The fact that borax contributes to the formation of a denser PVA-PVA network that inhibits electron conduction and lowers conductivity is crucial (7,5). On the other hand, studies based on maxin-borax hydrogel, despite the conductivity of maxin, did not show favorable results in terms of increasing conductivity (40,19). But, our PADMM-borax hydrogel showed the addition of AuNPs, and MB in the PADMM-borax hydrogel failed to show a significant effect on the conductivity because it passed the percolation network threshold, where the Denser of the borax network started to occur Fig.…”

The Use of Photoactive Polymeric Nanoparticles and Nanofibers to Generate a Photodynamic-Mediated Antimicrobial Effect, with a Special Emphasis on Chronic Wounds

“…Adsorption was achieved via the electrostatic interaction between the negative chitosan and the positive MB molecules, in addition to the chelating force of chitosan. Thus, the developed system exhibited a potent antibacterial activity compared to its individual components [ 93 ]. Table 1 summarizes some of the recently conducted studies where chitosan nanoparticles synergized the antimicrobial activity of PDT.…”