Biological potential of silver nanoparticles synthesized by an endophytic fungus Metapochonia suchlasporia-KUMBMDBT-23

Abstract

In this study, we demonstrated a simple, green, and eco-friendly method for the synthesis of silver nanoparticles using the endophytic fungus Metapochonia suchlasporia-KUMBMDBT-23 isolated from the medicinal plant Argemone mexicana. Silver nanoparticles were synthesised using the biomass cell filtrate of Metapochonia suchlasporia, which was identified by morphological and molecular characterization. The fungus extract was extracted with ethyl acetate (1:1 V/V) and was analysed by GC-MS. Biosynthesized silver nanoparticles were investigated by Bio-spectrophotometer, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy – Energy Dispersive Analysis of X-ray, (SEM-EDAX), X-ray diffraction (XRD) and Particle size analysis by dynamic light scattering (DLS). Metapochonia suchlasporia synthesised silver nanoparticles were effective against gram-positive and gram-negative human pathogenic bacteria. Using market antibiotics, the antimicrobial enhancing activity and minimum inhibitory concentration of silver nanoparticles was evaluated. The antifungal activity of silver nanoparticles against fungal infections was determined. Moreover, silver nanoparticles synthesised showed effect of DPPH and ABTS scavenging activities. Further in -vitro anticancer activity was determined by MTT assay using HePG2 and A498 cell lines. In -vitro anti-inflammatory activity was determined by egg albumin, bovine serum albumin denaturation test and membrane stabilization test, HRBC, in- vivo anti-inflammatory activity was determined by inducing Female Swiss Albino mice with inflammatory agent’s carrageenan paw edema. This study showed silver nanoparticles generated by microbes are effective inhibitors and have potential biological implications.