Biological Nitrogen Fixation (BNF) in Non-Leguminous Plants: the Role of Endophytic Diazotrophs

Baldani, J. I.; Oliveira, André L.M.; Guimarães, Salomão Lima; Baldani, V. L. D.; Reis, Fábio Bueno dos; Silva, L. G.; Reis, Verônica Massena; Teixeira, K. R. dos S.; Döbereiner, Johanna

doi:10.1007/0-306-47615-0_216

Cited by 12 publications

(4 citation statements)

References 17 publications

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“…strain STM678). Burkholderia tropicalis (strain PPe8), isolated from sugar cane (Baldani 1996; Baldani et al . 2000), showed high identity with B. brasilensis (98%), B. kururiensis (98%) and Burkholderia sp.…”

Section: Resultsmentioning

confidence: 99%

Characterization of amplified polymerase chain reaction glnB and nifH gene fragments of nitrogen-fixing Burkholderia species

Marin

Teixeira

Baldani

2003

Lett Appl Microbiol

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Aims: To clone and sequence polymerase chain reaction (PCR)-amplified glnB and nifH genes of the nitrogenfixing bacteria Burkholderia brasilensis strain M130, B. tropicalis strain PPe8 and B. kururiensis strain KP23. Methods and Results: The glnB and nifH gene fragments were amplified by PCR using universal degenerated primers. A very high percentage of similarity for the nifH (100%) and glnB (96%) genes was observed between strains M130 and KP23. A similarity of 100% for the nifH gene was also observed between strains M130 and PPe8. However, the identity for the glnB gene was 98% and the similarity 88%. The phylogenetic tree of the nifH gene showed a very high degree of similarity to the 16S rDNA gene. Conclusions:The nitrogen-fixing bacteria of the Burkholderia genus formed a cluster separated from the other species of the genus mainly when the nifH rather than the glnB gene was used to construct the phylogenetic tree. Significance and Impact of the Study: Knowledge of the nifH and glnB gene sequences of B. brasilensis, B. tropicalis and B. kururiensis will support new studies on the diversity of these diazotrophs in natural environments.

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Section: Resultsmentioning

confidence: 99%

Characterization of amplified polymerase chain reaction glnB and nifH gene fragments of nitrogen-fixing Burkholderia species

Marin

Teixeira

Baldani

2003

Lett Appl Microbiol

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“…In rice and maize, BNF contribution is similarly derived from a number of different species including members of Azospirillum, Azoarcus, Herbaspirillum, Bacillus, Enterobacter, Klebsiella and Pseudomonas (Boddey et al, 1995;Hurek et al, 1994;Kirchhof et al, 1998;Monteiro et al, 2012a). In field experiments using wild rice, grain yields increased to the equivalent of using an additional nitrogen fertilizer application of 40 kg N/ha following inoculation with Herbaspirillum seropedicea (Baldani et al, 2000;Pereira and Baldani, 1995). In another study, up to 30% of the total nitrogen accumulated in rice plants was derived from BNF, again demonstrating the potential gains to be made from bacterial associations (Malik et al, 1997).…”

Section: Nutrient Acquisitionmentioning

confidence: 99%

Understanding and engineering beneficial plant–microbe interactions: plant growth promotion in energy crops

Farrar

Bryant

Cope-Selby

2014

Plant Biotechnology Journal

241

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Plant production systems globally must be optimized to produce stable high yields from limited land under changing and variable climates. Demands for food, animal feed, and feedstocks for bioenergy and biorefining applications, are increasing with population growth, urbanization and affluence. Low-input, sustainable, alternatives to petrochemical-derived fertilizers and pesticides are required to reduce input costs and maintain or increase yields, with potential biological solutions having an important role to play. In contrast to crops that have been bred for food, many bioenergy crops are largely undomesticated, and so there is an opportunity to harness beneficial plant–microbe relationships which may have been inadvertently lost through intensive crop breeding. Plant–microbe interactions span a wide range of relationships in which one or both of the organisms may have a beneficial, neutral or negative effect on the other partner. A relatively small number of beneficial plant–microbe interactions are well understood and already exploited; however, others remain understudied and represent an untapped reservoir for optimizing plant production. There may be near-term applications for bacterial strains as microbial biopesticides and biofertilizers to increase biomass yield from energy crops grown on land unsuitable for food production. Longer term aims involve the design of synthetic genetic circuits within and between the host and microbes to optimize plant production. A highly exciting prospect is that endosymbionts comprise a unique resource of reduced complexity microbial genomes with adaptive traits of great interest for a wide variety of applications.

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“…Endophytic nitrogen-fixing species of the genus Herbaspirillum colonize sugar cane plants and promote growth and productivity Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans are the two species often found associated with sugar cane, being detected in many varieties collected in Brazil, Australia, and India . Although both species are diazotrophic and can colonize sugar cane endophytically and stimulate the plant growth, H.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Herbaspirillum seropedicae Cultivated in the Presence of Sugar Cane Extract

et al. 2013

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Bacterial endophytes of the genus Herbaspirillum colonize sugar cane and can promote plant growth. The molecular mechanisms that mediate plant- H. seropedicae interaction are poorly understood. In this work, we used 2D-PAGE electrophoresis to identify H. seropedicae proteins differentially expressed at the log growth phase in the presence of sugar cane extract. The differentially expressed proteins were validated by RT qPCR. A total of 16 differential spots (1 exclusively expressed, 7 absent, 5 up- and 3 down-regulated) in the presence of 5% sugar cane extract were identified; thus the host extract is able to induce and repress specific genes of H. seropedicae. The differentially expressed proteins suggest that exposure to sugar cane extract induced metabolic changes and adaptations in H. seropedicae presumably in preparation to establish interaction with the plant.

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Biological Nitrogen Fixation (BNF) in Non-Leguminous Plants: the Role of Endophytic Diazotrophs

Cited by 12 publications

References 17 publications

Characterization of amplified polymerase chain reaction glnB and nifH gene fragments of nitrogen-fixing Burkholderia species

Characterization of amplified polymerase chain reaction glnB and nifH gene fragments of nitrogen-fixing Burkholderia species

Understanding and engineering beneficial plant–microbe interactions: plant growth promotion in energy crops

Proteomic Analysis of Herbaspirillum seropedicae Cultivated in the Presence of Sugar Cane Extract

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