Biological Management of Chrysanthemum Wilt  (Fusarium oxysporum f.sp. chrysanthemi

Shamsher, Alam; Hasmi, Safdar Kaiser; Aftab, Mo. Alam; Khan, R. U.

doi:10.20546/ijcmas.2020.906.081

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“…The soft rot in D. officinale caused by F. oxysporum is one of the main diseases in the productive areas of China. This pathogen harms plants’ roots, causing vascular bundle disease and plant death, which can occur along the entire growth period of D. officinale , resulting in great losses to production [ 39 , 40 , 41 , 42 ]. The seriously damaged crops reported at home and abroad are eggplant, sugarcane, blackberry, cotton, etc.…”

Section: Discussionmentioning

confidence: 99%

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Xiao

2021

Biology

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Soft rot causing Fusarium oxysporum is one of the most destructive diseases of Dendrobium officinale Kimura et Migo in China that reduces D. officinale yield and quality. A key challenge for an integrated management strategy for this disease is the rapid and accurate detection of F. oxysporum on D. officinale. Therefore, a new loop-mediated isothermal amplification (LAMP) assay was developed for this purpose. In this study, the primers were selected and designed using the translation elongation factor-1α (TEF-1α) gene region as the target DNA sequence in order to screen the best system of reaction of LAMP to detect F. oxysporum through optimizing different conditions of the LAMP reaction, including time, temperature, concentrations of MgSO4, and concentrations of inner and outer primers. The optimized system was able to efficiently amplify the target gene at 62 °C for 60 min with 1.2 μM internal primers, 0.4 μM external primers, 7 mM Mg2+, and 5 fg/µL minimum detection concentration of DNA for F. oxysporum. The amplified products could be detected with the naked eye after completion of the reaction with SYBR green I. We were better able to control the effect of soft rot in D. officinale using fungicides following a positive test result. Additionally, the control effect of synergism combinations against soft rot was higher than 75%. Thus, LAMP assays could detect F. oxysporum in infected tissues of D. officinale and soils in field, allowing for early diagnosis of the disease.

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Section: Discussionmentioning

confidence: 99%

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Xiao

2021

Biology

View full text Add to dashboard Cite

show abstract

Biological Management of Chrysanthemum Wilt (Fusarium oxysporum f.sp. chrysanthemi

Cited by 1 publication

References 0 publications

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

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