Biological importance of human amniotic membrane in tissue engineering and regenerative medicine

Hu, Zeming; Luo, Yang; Ni, Renhao; Hu, Yiwei; Yang, Fang; Du, Tianyu; Zhu, Yabin

doi:10.1016/j.mtbio.2023.100790

Cited by 6 publications

(3 citation statements)

References 174 publications

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“…The pro-angiogenic effect of AM may originate from PDGF, VEGF, and angiogenin secreted by mesenchymal cells ( Farhadihosseinabadi et al, 2018 ). On the other hand, fibronectin is able to promote angiogenesis by activating the ERK signaling pathway via interacting with PDGF, EGF, and bFGF ( Hu et al, 2023 ). Tsai et al (2007) have concluded that the upregulating the expression of integrin, platelet-endothelial cell adhesion molecule-1 (PECAM-1), and VE-cadherin adhesion molecules in the cultured endothelial cells make them a potential candidate as vascular grafts.…”

Section: Bio-functionality Of Amnionmentioning

confidence: 99%

“…The applications of AM tissue and AM-derived stem cells in different fields of TE and RM are well-covered in recent literature ( Jafari et al, 2021 ; Elkhenany et al, 2022 ; Hu et al, 2023 ). This review aims to comprehensively explain the advanced multifaceted therapeutic functions of hydrogels derived from AM or dAM for tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Amnion-derived hydrogels as a versatile platform for regenerative therapy: from lab to market

Kafili,

Niknejad,

Tamjid

et al. 2024

Front. Bioeng. Biotechnol.

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Section: Bio-functionality Of Amnionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Amnion-derived hydrogels as a versatile platform for regenerative therapy: from lab to market

Kafili,

Niknejad,

Tamjid

et al. 2024

Front. Bioeng. Biotechnol.

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“…Since mounting evidence has demonstrated the efficacy of different sources of stem cells [1][2][3][4] and the derived secretome [5][6][7][8], in recent years, perinatal stem cells have been considered as an alternative and available cell source for biomedical research in vitro [9,10] and in vivo [11], including clinical trials [12][13][14].…”

mentioning

confidence: 99%

Amniotic Fluid and Placental Membranes as Sources of Stem Cells: Progress and Challenges 2.0

Maraldi,

Russo

2023

IJMS

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A hybrid construct of decellularized matrix and fibrin for differentiating adipose stem cells into insulin‐producing cells, an optimized in vitro assessment

Bozorgi,

Khazaei,

Bozorgi

et al. 2024

Cell Biochemistry & Function

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The generation of insulin‐producing cells (IPCs) is an attractive approach for replacing damaged β cells in diabetic patients. In the present work, we introduced a hybrid platform of decellularized amniotic membrane (dAM) and fibrin encapsulation for differentiating adipose tissue‐derived stem cells (ASCs) into IPCs. ASCs were isolated from healthy donors and characterized. Human AM was decellularized, and its morphology, DNA, collagen, glycosaminoglycan (GAG) contents, and biocompatibility were evaluated. ASCs were subjected to four IPC differentiation methods, and the most efficient method was selected for the experiment. ASCs were seeded onto dAM, alone or encapsulated in fibrin gel with various thrombin concentrations, and differentiated into IPCs according to a method applying serum‐free media containing 2‐mercaptoethanol, nicotinamide, and exendin‐4. PDX‐1, GLUT‐2 and insulin expression were evaluated in differentiated cells using real‐time PCR. Structural integrity and collagen and GAG contents of AM were preserved after decellularization, while DNA content was minimized. Cultivating ASCs on dAM augmented their attachment, proliferation, and viability and enhanced the expression of PDX‐1, GLUT‐2, and insulin in differentiated cells. Encapsulating ASCs in fibrin gel containing 2 mg/ml fibrinogen and 10 units/ml thrombin increased their differentiation into IPCs. dAM and fibrin gel synergistically enhanced the differentiation of ASCs into IPCs, which could be considered an appropriate strategy for replacing damaged β cells.

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Biological importance of human amniotic membrane in tissue engineering and regenerative medicine

Cited by 6 publications

References 174 publications

Amnion-derived hydrogels as a versatile platform for regenerative therapy: from lab to market

Amnion-derived hydrogels as a versatile platform for regenerative therapy: from lab to market

Amniotic Fluid and Placental Membranes as Sources of Stem Cells: Progress and Challenges 2.0

A hybrid construct of decellularized matrix and fibrin for differentiating adipose stem cells into insulin‐producing cells, an optimized in vitro assessment

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