Amniotic Fluid and Placental Membranes as Sources of Stem Cells: Progress and Challenges 2.0

Maraldi, Tullia; Russo, Valentina

doi:10.3390/ijms242216020

IJMS

2023

DOI: 10.3390/ijms242216020

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Amniotic Fluid and Placental Membranes as Sources of Stem Cells: Progress and Challenges 2.0

Tullia Maraldi,

Valentina Russo

Abstract: The aim of the second edition of this Special Issue was to collect both review and original research articles that investigate and elucidate the possible therapeutic role of perinatal stem cells in pathological conditions, such as cardiovascular and metabolic diseases, as well as inflammatory, autoimmune, musculoskeletal, and degenerative diseases [...]

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2024

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Comparative in‐vitro analysis of amniotic Fluid's efficacy in sustaining viability and regulating apoptosis of periodontal fibroblasts versus HBSS

Harjai,

Shanbhog

2024

Dental Traumatology

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BackgroundTooth avulsion necessitates swift replantation, for which the preservation of periodontal ligament (PDL) cell viability is paramount. Various storage media have been explored, yet a comparison between amniotic fluid (AF) obtained at different gestational stages (amniocentesis and full‐term) and HBSS is lacking.AimThis study aims to evaluate AF (amniocentesis and full‐term) against HBSS in sustaining PDL cell viability and regulating apoptosis at different time points.Material and MethodsPeriodontal fibroblasts cultured in α‐MEM were treated with 100% AF (amniocentesis), 100% AF (full‐term), and HBSS, incubated for 1, 3, 24, and 48 h at 37°C, and assessed using the MTT assay for viability and AO/EB staining for apoptosis, which was analyzed via fluorescent microscopy after 24 h. Statistical analysis was conducted using one‐way ANOVA, multivariate ANOVA, and post hoc Tukey's multiple comparison tests (p < .05).ResultsAmniotic fluid (amniocentesis) exhibited the highest optical density (OD), which implies the highest cell viability across time intervals, followed by AF (full‐term) and HBSS. While HBSS maintained PDL morphology, both AF groups showed altered morphology. No cell death was observed after 24 h.ConclusionsWithin the limitations of this study, both AF groups showed the potential to sustain PDL cell viability after 1, 3, 24, and 48 h of storage. However, further investigation is warranted regarding their suitability as storage media.

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Comparative in‐vitro analysis of amniotic Fluid's efficacy in sustaining viability and regulating apoptosis of periodontal fibroblasts versus HBSS

Harjai,

Shanbhog

2024

Dental Traumatology

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Amniotic Fluid and Placental Membranes as Sources of Stem Cells: Progress and Challenges 2.0

Cited by 1 publication

References 41 publications

Comparative in‐vitro analysis of amniotic Fluid's efficacy in sustaining viability and regulating apoptosis of periodontal fibroblasts versus HBSS

Comparative in‐vitro analysis of amniotic Fluid's efficacy in sustaining viability and regulating apoptosis of periodontal fibroblasts versus HBSS

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