Biological functionalization of dental implants with collagen and glycosaminoglycans—A comparative study

Stadlinger, Bernd; Hintze, Vera; Bierbaum, Susanne; Möller, Stephanie; Schulz, Matthias C.; Mai, Ronald; Kuhlisch, Eberhard; Heinemann, Sascha; Scharnweber, Dieter; Schnabelrauch, Matthias; Eckelt, Uwe

doi:10.1002/jbm.b.31953

Cited by 53 publications

(55 citation statements)

References 48 publications

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“…From a minipig study evaluating the submerged healing of dental implants in the maxilla (Stadlinger et al, 2011), six implant/host bone samples were randomly selected from different animals after a two-month healing period. The study protocol was approved by the commission for animal studies at the District Government Offi ce, Dresden, Germany.…”

Section: Methodsmentioning

confidence: 99%

Comparison of bone-implant contact and bone-implant volume between 2D-histological sections and 3D-SRµCT slices

Bernhardt

Kuhlisch

Schulz

et al. 2012

eCM

103

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Histological imaging is still considered the gold standard for analysing bone formation around metallic implants. Generally, a limited number of histological sections per sample are used for the approximation of mean values of peri-implant bone formation. In this study we compared statistically the results of bone-implant contact (BIC) and bone-implant volume (BIV) obtained by histological sections, with those obtained by X-ray absorption images from synchrotron radiation micro-computed tomography (SRμCT) using osseointegrated screw-shaped implants from a mini-pig study. Comparing the BIC results of 3-4 histological sections per implant sample with the appropriate 3-4 SRμCT slices showed a non-signifi cant difference of 1.9 % (p = 0.703). The contact area assessed by the whole 3D information from the SRμCT measurement in comparison to the histomorphometric results showed a non-signifi cant difference in BIC of 4.9 % (p = 0.171). The amount of the bone-implant volume in the histological sections and the appropriate SRμCT slices showed a nonsignifi cant difference by only 1.4 % (p = 0.736) and also remains non-signifi cant with 2.6 % (p = 0.323) using the volumetric SRμCT information. We conclude that for a clinical evaluation of implant osseointegration with histological imaging at least 3-4 sections per sample are suffi cient to represent the BIC or BIV for a sample. Due to the fact that in this study we have found a signifi cant intra-sample variation in BIC of up to ± 35 % the selection of only one or two histological sections per sample may strongly infl uence the determined BIC.

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Section: Methodsmentioning

confidence: 99%

Comparison of bone-implant contact and bone-implant volume between 2D-histological sections and 3D-SRµCT slices

Bernhardt

Kuhlisch

Schulz

et al. 2012

eCM

103

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“…Six titanium coated polyetheretherketone (PEEK) implants of a special design-to create a defined area between the implant surface and the round drill hole reported in [24,45]-were inserted in the mandibular bone of minipigs. The mandibles were resected after healing periods of two, four, and eight weeks.…”

Section: Sample Preparationmentioning

confidence: 99%

Early Stages of Biomineral Formation—A Solid-State NMR Investigation of the Mandibles of Minipigs

et al. 2017

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Solid-state nuclear magnetic resonance (NMR) spectroscopy allows for the identification of inorganic species during the biomineral formation, when crystallite particles visible in direct imaging techniques have not yet been formed. The bone blocks surrounding dental implants in minipigs were dissected after the healing periods of two, four, and eight weeks, and newly formed tissues formed around the implants were investigated ex vivo. Two-dimensional 31 P-1 H heteronuclear correlation (HETCOR) spectroscopy is based on the distance-dependent heteronuclear dipolar coupling between phosphate-and hydrogen-containing species and provides sufficient spectral resolution for the identification of different phosphate minerals. The nature of inorganic species present at different mineralization stages has been determined based on the 31 P chemical shift information. After a healing time of two weeks, pre-stages of mineralization with a rather unstructured distribution of structural motives were found. After four weeks, different structures, which can be described as nanocrystals exhibiting a high surface-to-volume ratio were detected. They grew and, after eight weeks, showed chemical structures similar to those of matured bone. In addition to hydroxyapatite, amorphous calcium phosphate, and octacalcium phosphate, observed in a reference sample of mature bone, signatures of ß-tricalcium phosphate and brushite-like structures were determined at the earlier stages of bone healing.

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“…It is difficult to speculate on the why as the actual processes and interactions that take place during their osseointegration are not completely understood. 31 One reason may be the added structural effects of such coatings, as morphological changes are known to influence cell behavior, 32 and both peptide and GF coatings do not contribute to this aspect. Another possibility is direct and receptor-specific interactions between the coatings and cells.…”

Section: Effect Of Coatingmentioning

confidence: 99%

A systematic review and meta‐analysis on the influence of biological implant surface coatings on periimplant bone formation

Jenny

Jauernik

Bierbaum

et al. 2016

J Biomedical Materials Res

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This systematic review and meta-analysis evaluated the influence of biological implant surface coatings on periimplant bone formation in comparison to an uncoated titanium reference surface in experimental large animal models. The analysis was structured according to the PRISMA criteriae. Of the1077 studies, 30 studies met the inclusion criteriae. Nineteen studies examined the bone implant contact (BIC) and were included in the meta-analysis. Overall, the mean increase in BIC for the test surfaces compared to the reference surfaces was 3.7 percentage points (pp) (95% CI -3.9-11.2, p = 0.339). Analyzing the increase in BIC for specific coated surfaces in comparison to uncoated reference surfaces, inorganic surface coatings showed a significant mean increase in BIC of 14.7 pp (95% CI 10.6-18.9, p < 0.01), extracellular matrix (ECM) surface coatings showed an increase of 10.0 pp (95% CI 4.4-15.6, p < 0.001), and peptide coatings showed a statistical trend with 7.1 pp BIC increase (95% CI -0.8-15.0, p = 0.08). In this review, no statistically significant difference could be found for growth factor surface coatings (observed difference -3.3 pp, 95% CI -16.5-9.9, p = 0.6). All analyses are exploratory in nature. The results show a statistically significant effect of inorganic and ECM coatings on periimplant bone formation. Abstract: This systematic review and meta-analysis evaluated the influence of biological implant surface coatings on periimplant bone formation in comparison to an uncoated titanium reference surface in experimental large animal models. The analysis was structured according to the PRISMA criteriae. Of the1077 studies, 30 studies met the inclusion criteriae. Nineteen studies examined the bone implant contact (BIC) and were included in the meta-analysis. Overall, the mean increase in BIC for the test surfaces compared to the reference surfaces was 3.7 percentage points (pp) (95% CI 23.9-11.2, p 5 0.339). Analyzing the increase in BIC for specific coated surfaces in comparison to uncoated reference surfaces, inorganic surface coatings showed a significant mean increase in BIC of 14.7 pp (95% CI 10.6-18.9, p < 0.01), extracellular matrix (ECM) surface coatings showed an increase of 10.0 pp (95% CI 4.4-15.6, p < 0.001), and peptide coatings showed a statistical trend with 7.1 pp BIC increase (95% CI 20.8-15.0, p 5 0.08). In this review, no statistically significant difference could be found for growth factor surface coatings (observed difference 23.3 pp, 95% CI 216.5-9.9, p 5 0.6). All analyses are exploratory in nature. The results show a statistically significant effect of inorganic and ECM coatings on periimplant bone formation.

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Biological functionalization of dental implants with collagen and glycosaminoglycans—A comparative study

Cited by 53 publications

References 48 publications

Comparison of bone-implant contact and bone-implant volume between 2D-histological sections and 3D-SRµCT slices

Comparison of bone-implant contact and bone-implant volume between 2D-histological sections and 3D-SRµCT slices

Early Stages of Biomineral Formation—A Solid-State NMR Investigation of the Mandibles of Minipigs

A systematic review and meta‐analysis on the influence of biological implant surface coatings on periimplant bone formation

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