Biological Evaluation of Polyvinyl Alcohol Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite

Chocholata, Petra; Kulda, Vlastimil; Dvořáková, Jana; Dobrá, Jana; Babuška, Václav

doi:10.3390/ijms21165719

Cited by 29 publications

(34 citation statements)

References 24 publications

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“…There are created cellseeded three-dimensional scaffolds, which are inserted into the environment of the human body. Materials used in bone tissue engineering for the scaffolds should be as similar as possible to human tissues [3,4].…”

Section: Introductionmentioning

confidence: 99%

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In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

Chocholata

Kulda

Dvořáková

et al. 2021

IJMS

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Bone tissue engineering tries to simulate natural behavior of hard tissues. This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp. In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity. The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp. For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001). It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.

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Section: Introductionmentioning

confidence: 99%

“…Hydrogels are three-dimensional hydrophilic biopolymeric networks that can tightly absorb and retain large quantities of water or a biological fluid without dissolving in their swollen state [3,5,6]. According to the origin of the polymers of which they are composed, hydrogels can be divided into natural, synthetic or hybrid.…”

Section: Introductionmentioning

confidence: 99%

In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

Chocholata

Kulda

Dvořáková

et al. 2021

IJMS

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“…In order to confirm the cytotoxicity of the high concentration of the vancomycin (50 mg/mL) and gentamicin (25 mg/mL) used to medicate the scaffolds, a preliminary 2D in vitro study was carried out using the MG63 osteosarcoma cell line as a simplified model of an osteoblast-like cellular phenotype; it is widely selected for various cytotoxicity tests [62][63][64][65]. The quantitative MTT assay was performed in order to evaluate cell viability and proliferation in the presence of the two free antibiotics.…”

Section: Biological Evaluationsmentioning

confidence: 99%

Medicated Hydroxyapatite/Collagen Hybrid Scaffolds for Bone Regeneration and Local Antimicrobial Therapy to Prevent Bone Infections

et al. 2021

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Microbial infections occurring during bone surgical treatment, the cause of osteomyelitis and implant failures, are still an open challenge in orthopedics. Conventional therapies are often ineffective and associated with serious side effects due to the amount of drugs administered by systemic routes. In this study, a medicated osteoinductive and bioresorbable bone graft was designed and investigated for its ability to control antibiotic drug release in situ. This represents an ideal solution for the eradication or prevention of infection, while simultaneously repairing bone defects. Vancomycin hydrochloride and gentamicin sulfate, here considered for testing, were loaded into a previously developed and largely investigated hybrid bone-mimetic scaffold made of collagen fibers biomineralized with magnesium doped-hydroxyapatite (MgHA/Coll), which in the last ten years has widely demonstrated its effective potential in bone tissue regeneration. Here, we have explored whether it can be used as a controlled local delivery system for antibiotic drugs. An easy loading method was selected in order to be reproducible, quickly, in the operating room. The maintenance of the antibacterial efficiency of the released drugs and the biosafety of medicated scaffolds were assessed with microbiological and in vitro tests, which demonstrated that the MgHA/Coll scaffolds were safe and effective as a local delivery system for an extended duration therapy—promising results for the prevention of bone defect-related infections in orthopedic surgeries.

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“…HYH was also combined with other biopolymers in order to improve porosity and biocompatibility [ 67 , 70 , 71 , 72 , 73 ]. HYH–Chitosan–Collagen–HAP scaffolds have been developed with a porosity of over 90%.…”

Section: Hyh–hap and Hyh–cap Compositesmentioning

confidence: 99%

Hyaluronic-Acid-Based Organic-Inorganic Composites for Biomedical Applications

2021

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Applications of natural hyaluronic acid (HYH) for the fabrication of organic–inorganic composites for biomedical applications are described. Such composites combine unique functional properties of HYH with functional properties of hydroxyapatite, various bioceramics, bioglass, biocements, metal nanoparticles, and quantum dots. Functional properties of advanced composite gels, scaffold materials, cements, particles, films, and coatings are described. Benefiting from the synergy of properties of HYH and inorganic components, advanced composites provide a platform for the development of new drug delivery materials. Many advanced properties of composites are attributed to the ability of HYH to promote biomineralization. Properties of HYH are a key factor for the development of colloidal and electrochemical methods for the fabrication of films and protective coatings for surface modification of biomedical implants and the development of advanced biosensors. Overcoming limitations of traditional materials, HYH is used as a biocompatible capping, dispersing, and structure-directing agent for the synthesis of functional inorganic materials and composites. Gel-forming properties of HYH enable a facile and straightforward approach to the fabrication of antimicrobial materials in different forms. Of particular interest are applications of HYH for the fabrication of biosensors. This review summarizes manufacturing strategies and mechanisms and outlines future trends in the development of functional biocomposites.

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Biological Evaluation of Polyvinyl Alcohol Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite

Cited by 29 publications

References 24 publications

In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

Medicated Hydroxyapatite/Collagen Hybrid Scaffolds for Bone Regeneration and Local Antimicrobial Therapy to Prevent Bone Infections

Hyaluronic-Acid-Based Organic-Inorganic Composites for Biomedical Applications

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