Biological evaluation of an ionomeric bone cement by osteoblast cell culture methods

Meyer, Ulrich; Szulczewski, Dale H.; Barckhaus, R.H.; Atkinson, Michael J.; Jones, David B.

doi:10.1016/0142-9612(93)90133-m

Cited by 38 publications

(19 citation statements)

References 32 publications

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“…Such adhesive properties are important for cell proliferation and differentiation into bone forming cells. 35 Osteoblasts cultured on zirconia and alumina presented similar morphological features with polygonal aspects compared to those on glass coverslips. Similar observations were reported by Malik et al 34 and Howlett et al 10 A number of studies focused on the mechanism of initial attachment of bone-derived cells on different materials such as ceramics and metallic surfaces.…”

Section: Discussionmentioning

confidence: 83%

“…34 Many models are based on the ability of osteoblasts to migrate and colonize foreign materials. 35 To our knowledge, very little is known about the behavior of cells in culture with zirconia 14,17,19 and alumina. 9,10,17,20 In this work we used osteoblast cell culture models obtained from human bone explantation to mimic the cellular environment at the implant site of an in vivo situation.…”

Section: Discussionmentioning

confidence: 99%

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In vitro reactions of human osteoblasts in culture with zirconia and alumina ceramics

Josset

Oum'Hamed

Zarrinpour

et al. 1999

J. Biomed. Mater. Res.

145

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The biocompatibility of two implantable materials, zirconia and alumina ceramics, was investigated in vitro using human osteoblast cell cultures. The viability of osteoblast cells with the materials was evaluated by the methylthiazole sulfate test that revealed an absence of any cytostatic or cytotoxic effect. Cell proliferation kinetic and total protein synthesis in osteoblasts with zirconia or alumina were similar to that observed in control cells cultured on glass coverslips. Light and scanning electron microscopic examinations showed an intimate contact between osteoblasts and the substrates; well-spread cells were observed on the surfaces of both materials. Adhesion ability and morphological characteristics were preserved in osteoblast cultures with these substrates. Moreover, immunohistochemical staining in osteoblasts with zirconia and alumina showed the capacity of these cells to elaborate the extracellular matrix composed of types I and V collagen, osteocalcin, osteonectin, bone sialoprotein, and cellular fibronectin. Finally, DNA image cytometry and interphase silver-nucleolar organizer regions quantification were applied as complementary biocompatibility tests to detect any changes in DNA synthesis and cell proliferation, respectively. The results showed that neither material altered cell ploidy or cell growth rate in accordance with the absence of any inducing effect on DNA synthesis or proliferation.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

In vitro reactions of human osteoblasts in culture with zirconia and alumina ceramics

Josset

Oum'Hamed

Zarrinpour

et al. 1999

J. Biomed. Mater. Res.

145

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“…16 Nevertheless, for a cement to be adequate for clinical use, its components must be biocompatible. 15,[17][18][19][20][21] In this context, the aim of the present experiment was to show evidence of the action of different dental ionomers on tissues, by means of histological analysis. This analysis must fundamentally be based on how live vascularized tissue is influenced by aggression, which may be associated with cell death and necrosis, and how this aggression influences the developmental characteristics of capsule collagenization, inflammatory infiltrate and macrophage action.…”

Section: Discussionmentioning

confidence: 99%

“…7 These acids have been pointed out as being substances capable of causing oxidative stress within the cells and interfering in cellular response. 19 Moreover, the low pH of these materials, especially when recently prepared, may have an influence on the potential inflammatory response, [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] as supported by Ribeiro et al 7 These authors demonstrated that Ketac Cem cement powder did not induce alteration in cell DNA, whereas measurable genotoxicity was found for Ketac Cem liquid, composed of tartaric and benzoic acid, in all the dilutions tested in cell cultures.…”

Section: Discussionmentioning

confidence: 99%

“…15,22 In addition to the inflammatory infiltrate, circulatory alterations (edema) and multinucleated giant cells proved expressively greater with the Meron cement at 7 days, contrary to the respective results observed for the other cements. This was a proportional response of the body to the presence of aluminum and/or iron ions, [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] whereby giant cells are released in the tissue to promote phagocytosis, a process by which the ions are surrounded and contained. Moreover, leachable substances from the GICs may also affect the epithelial cell rate of progression through the cellular cycle.…”

Section: Discussionmentioning

confidence: 99%

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Histological analysis of biocompatibility of ionomer cements with an acid-base reaction

et al. 2014

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histological analysis of biocompatibility of ionomer cements with an acid-base reaction abstract: The purpose of this study was to evaluate the inflammatory and cure events of acid-based reactions using glass ionomer cement used for cementation of crowns, bridges, onlays and orthodontic bands implanted in subcutaneous tissue, at different time intervals. A total of 48 male Wistar rats were used, distributed into 4 groups (n = 12), as follows: Group C (control, polyethylene), Group ME (Meron), Group KC (Ketac Cem) and Group PR (Precedent). The animals were sacrificed after time intervals of 7, 15 and 30 days, and their tissues were analyzed under an optical microscope for such events as inflammatory infiltrate, edema, necrosis, granulation tissue, multinucleated giant cells, young fibroblasts and collagen. The results was assessed using Kruskal-Wallis and Dunn's tests (p < 0.05). In the initial period, intense inflammatory infiltrate was observed for all the materials with no significant difference among them (p = 0.104). Groups PR and KC showed significant difference in relation to Group C, at 7 days (p = 0.025) and 15 days (p = 0.006). Edema and giant cells were more expressive in Group ME, differing significantly from Groups C (p = 0.023) and KC (p = 0.039), respectively, at 7 days. Group ME showed a statistically significant difference in relation to Groups PR and KC for the presence of young fibroblasts (p = 0.009) and for collagen (p = 0.002), at 7 days. Within the limits of this in vivo study, Precedent and Ketac Cem glass ionomer cements showed better tissue healing with a greater number of fibroblasts and collagen, as compared to Meron.

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Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses

Anselme

Bigerelle

Noël

et al. 2000

J. Biomed. Mater. Res.

134

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We quantitatively evaluated the adhesion of human osteoblasts on orthopedic metallic substrates (Ti6Al4V alloy) with various surface roughnesses at several times after inoculation and studied its correlation with qualitative changes in the expression of adhesion proteins and with parameters extensively describing the surface topographies. Cells were orientated in a parallel order on polished surfaces. This orientation was not affected by residual grooves after polishing. On sandblasted surfaces the cells never attained confluence and had a stellate shape, and the cell layer had no particular organization. Extracellular matrix (fibronectin, type I collagen, osteopontin) and cytoskeletal protein (actin, vinculin) orientation reflected the cell layer organization. In our experiment human osteoblasts expressed ␣3␤1 integrin but not ␣2␤1 integrin. In addition to currently ana-lyzed roughness magnitude parameters, we calculated roughness organization parameters (fractal dimension parameters) of the substrates. We observed lower adhesion and proliferation on less organized surfaces (i.e., sandblasted ones). The significant statistical correlation observed between fractal dimension parameters (describing surface roughness organization) and cell parameters adds a new concept to the studies of substratum roughness influence on cell behavior. An attempt at modelization of the cell-surface interaction was made that includes the influence of fractal dimensions parameters.

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Biological evaluation of an ionomeric bone cement by osteoblast cell culture methods

Cited by 38 publications

References 32 publications

In vitro reactions of human osteoblasts in culture with zirconia and alumina ceramics

In vitro reactions of human osteoblasts in culture with zirconia and alumina ceramics

Histological analysis of biocompatibility of ionomer cements with an acid-base reaction

Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses

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