Biological effects of narrow-band (311 nm TL01) UVB irradiation: a review

El‐Ghorr, Ali A.; Norval, Mary

doi:10.1016/s1011-1344(96)07454-4

Cited by 136 publications

(127 citation statements)

References 36 publications

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“…In healthy volunteers, a significant decrease in epidermal LCs count was observed post broad-and narrowband UVB exposure, without a significant difference in effect between them [38]. In previous studies, BB-UVB resulted in stronger reduction in LCs density in murine epidermis than NB-UVB irradiation [39]. …”

Section: Discussionmentioning

confidence: 75%

Clinical immunology Density of Langerhans cells in chronic plaque psoriatic lesions before and after phototherapy

Rotsztejn¹,

Chomiczewska²,

Trznadel-Grodzka³

et al. 2012

cejoi

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Section: Discussionmentioning

confidence: 75%

Clinical immunology Density of Langerhans cells in chronic plaque psoriatic lesions before and after phototherapy

Rotsztejn¹,

Chomiczewska²,

Trznadel-Grodzka³

et al. 2012

cejoi

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“…(10)(11)(12)(13) Narrowband UVB is widely used in the treatment of skin disease, and the current trend toward the increased use of narrowband UVB phototherapy is justified. (14) Its carcinogenic potential is judged to be substantially less than that of psoralen plus UVA (PUVA) photochemotherapy. (15) Although the results of studies in mice indicate that narrowband UVB could induce more skin cancers than broadband UVB therapy, (16) the participants in a workshop on the use of narrowband UVB in phototherapy concluded that the long-term human cancer risk should be no greater than that with broadband phototherapy.…”

mentioning

confidence: 99%

Formation of 8‐hydroxy‐2′‐deoxyguanosine in the DNA of cultured human keratinocytes by clinically used doses of narrowband and broadband ultraviolet B and psoralen plus ultraviolet A

et al. 2006

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Psoralen plus ultraviolet A (PUVA) and narrowband ultraviolet B (UVB) are widely used in skin disease phototherapy. Recently, the efficacy of UVB therapy has been greatly improved by narrowband UVB, compared to conventional broadband UVB. The objectives of the current study were to evaluate the influence of UVB-induced and PUVA-induced oxidative stress on cultured keratinocytes. We analyzed 8-hydroxy-2′ ′ ′ ′-deoxyguanosine (8-OH-dG) in human keratinocytes (HaCaT cell line) using a highperformance liquid chromatography system equipped with an electrochemical detector. Non-irradiated human keratinocytes contained a baseline of 1.48 ± ± ± ± 0.22 (mean ± ± ± ± SD) 8-OH-dG per 10 6 deoxyguanosine (dG) residues in cellular DNA, which increased linearly with higher doses of UVB. When their abilities to induce 8-OH-dG were compared to each other, based on the minimal erythemal and therapeutically used doses, by irradiating them with broadband UVB at 100 mJ/cm 2 , the amount of 8-OH-dG increased to 3.42 ± ± ± ± 0.46 residues per 10 6 dG, while a narrowband UVB treatment at 1000 mJ/cm 2 , with biological effects comparable to those elicited by 100 mJ/cm 2 broadband UVB, increased it to 2.06 ± ± ± ± 0.31 residues per 10 6 dG. PUVA treatment, with 100 ng/mL 8-methoxypsoralen and 5000 mJ/cm 2 UVA, increased the 8-OH-dG level to 4.52 ± ± ± ± 0.42 residues per 10 6 dG. When HaCaT cells treated with 2000 mJ/cm 2 narrowband UVB were cultured and the amount of 8-OH-dG was monitored in the living cells, 65.6% of the residues were repaired 24 h after treatment. Our study provides a warning that widely used narrowband UVB and PUVA induce cellular oxidative DNA damage at the therapeutically used doses, although to a lesser degree than broadband UVB with the same clinically effective dose. (Cancer Sci 2006; 97: 99 -105) E ight-hydroxy-2′-deoxyguanosine (8-OH-dG), also known as 7,8-dihydro-8-oxo-deoxyguanosine (8-oxo-dG), (1) has been proposed as a key biomarker of oxidative DNA damage relevant to carcinogenesis (1,2) and pathogenesis of autoimmune disorders. (3,4) This DNA damage is induced by the reactions of reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ), superoxide anions ( ), singlet oxygen and hydroxyl radicals (·OH).Human skin is constantly exposed to environmental stresses, and is vulnerable to the effects of ROS generated by exposure to ultraviolet (UV) radiation.(5) Yamamoto et al. (6) reported that the formation of 8-OH-dG in DNA might be one of the mechanisms of daylight-induced mutagenesis. In fact, irradiation with a fluorescent sun lamp or with UVB does induce 8-OH-dG in the epidermis of hairless mice. (7,8) Parrish and Jaenicke (9) found that 313 nm UVB radiation is the most effective wavelength for the treatment of psoriasis. This finding provided the impetus for developing the Philips TL-01 fluorescent bulb, a narrowband UVB light source that produces a spectral emission between 310 and 315 nm. Narrowband UVB phototherapy has thus significantly improved the therapeutic efficacy of conventional br...

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“…10 Narrowband UV-B was introduced recently for the treatment of psoriasis and found to be more effective than broadband UV-B at clearing psoriatic lesions. 11 Indeed, even when compared with PUVA photochemotherapy, narrowband UV-B was reported to have on average the same efficacy but fewer side effects. 12 Therefore, the purpose of our study was to investigate the effect of narrowband (311-nm) UV-B in patients with SPP and early-stage MF.…”

mentioning

confidence: 99%

Narrowband (311-nm) UV-B Therapy for Small Plaque Parapsoriasis and Early-Stage Mycosis Fungoides

Hofer

Cerroni²,

Kerl³

et al. 1999

Arch Dermatol

131

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Biological effects of narrow-band (311 nm TL01) UVB irradiation: a review

Cited by 136 publications

References 36 publications

Clinical immunology Density of Langerhans cells in chronic plaque psoriatic lesions before and after phototherapy

Clinical immunology Density of Langerhans cells in chronic plaque psoriatic lesions before and after phototherapy

Formation of 8‐hydroxy‐2′‐deoxyguanosine in the DNA of cultured human keratinocytes by clinically used doses of narrowband and broadband ultraviolet B and psoralen plus ultraviolet A

Narrowband (311-nm) UV-B Therapy for Small Plaque Parapsoriasis and Early-Stage Mycosis Fungoides

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