Clinical immunology Density of Langerhans cells in chronic plaque psoriatic lesions before and after phototherapy

Rotsztejn, Helena; Chomiczewska, Dorota; Trznadel-Grodzka, Ewa; Jesionek-Kupnicka, Dorota

doi:10.5114/ceji.2012.30803

Cited by 4 publications

(6 citation statements)

References 33 publications

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“…This is further complicated by conflicting results and whether treatment affects the number of LCs in psoriatic skin. 28,[54][55][56][57][58] However, in agreement with a study on the effect of sun on macrophages and dendritic cells, we found a reduction in both cell types. 33 The number of LCs was significantly increased in LS compared with NL skin at baseline.…”

Section: Discussionsupporting

confidence: 92%

Climatotherapy at the Dead Sea for psoriasis is a highly effective anti‐inflammatory treatment in the short term: An immunohistochemical study

Emmanuel

Petersen

Houborg

et al. 2022

Experimental Dermatology

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Climatotherapy is a well‐described treatment of psoriasis. Dead Sea climatotherapy (DSC) in Israel consists of intensive sun and Dead Sea bathing and is very effective in improving clinical and patient‐reported outcomes. However, the effect of DSC has not been widely studied. We aimed to investigate the effect of DSC on psoriasis skin using quantitative immunohistochemistry techniques and analysis of blood samples. Skin punch biopsies from 18 psoriasis patients from a previous cohort study were used. Biopsies were obtained from non‐lesional skin and from a psoriasis target lesion at baseline. A biopsy was acquired from the target lesion after DSC. Among patients who achieved complete visual clearance, a biopsy was also obtained at relapse. Blood samples were obtained at the same time points. We performed haematoxylin and eosin staining and quantitative immunohistochemical analysis of CD3, CD4, CD8, CD11c, CD103, CD163, CD207, forkhead box P3, Ki67 and myeloperoxidase. We performed blood tests of cholesterol, c‐reactive protein, glucose, haemoglobin A1c and triglycerides. All skin biomarkers except for CD207 were decreased after DSC. At relapse, none of the biomarkers were significantly different from the baseline lesional measurements. Total CD207 staining correlated with psoriasis area and severity index at baseline while CD163 staining correlated with psoriasis area and severity index at EOT. No changes were observed in selected blood tests during the study. Consistent with clinical results, DSC is highly effective in the short term almost normalising all investigated biomarkers. However, at relapse, biomarkers were upregulated to the baseline level.

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Section: Discussionsupporting

confidence: 92%

Climatotherapy at the Dead Sea for psoriasis is a highly effective anti‐inflammatory treatment in the short term: An immunohistochemical study

Emmanuel

Petersen

Houborg

et al. 2022

Experimental Dermatology

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“…The level of LC has previously been reported to be downregulated in lesional psoriatic skin, but discrepancies in the reported results exist . These discrepancies could be due to the use of different quantification methods.…”

Section: Discussionmentioning

confidence: 84%

“…The role of LC in the pathogenesis of psoriasis remains unknown. It has been shown that the number of LC is downregulated in lesional psoriatic skin, but conflicting results exist . Moreover, the depletion of LC in psoriasis aggravated psoriasis symptoms during ongoing disease in a mouse model where psoriasis‐like skin diseases were mimicked by deletion of Jun and JunB genes via a tamoxifen‐inducible cre recombinase in keratin‐5‐expressing cells .…”

Section: Introductionmentioning

confidence: 99%

Langerhans cell markers CD1a and CD207 are the most rapidly responding genes in lesional psoriatic skin following adalimumab treatment

Raaby

Rosada

Langkilde

et al. 2017

Experimental Dermatology

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TNFα-, IL-23- and IL-17-targeting drugs are highly effective in the treatment of psoriasis. However, the precise molecular mechanism remains unknown. In psoriatic skin, the presence of Langerhans cells (LCs) is reduced, but the role of LC is poorly understood. The purpose of this study was to investigate the impact of TNFα and IL-23/IL-17 on the presence of LC in the skin during treatment. Therefore, psoriatic skin was investigated before and after 4 days of adalimumab or ustekinumab treatment. Furthermore, TNFα and IL-17A stimulation was investigated in an ex vivo model of epidermis and dermis from healthy normal skin kept in cultures at an air-liquid interphase for 4 days. In a gene array analysis, we found that the two LC markers, CD1a and CD207, were among the most up- or downregulated genes in psoriatic skin after anti-TNFα therapy. Validation showed that both mRNA expression and protein level followed the same pattern and became significantly upregulated after 4 days of treatment. No changes were seen after ustekinumab treatment. In the ex vivo skin model, a decrease in the CD1a level was seen after TNFα stimulation and it was caused by LC migration from epidermis. No response in LC migration was seen after IL-17A stimulation. Taken together, we demonstrated that changes in the LC level in epidermis precede the histological and clinical changes during adalimumab treatment in psoriatic skin. Furthermore, TNFα plays a prominent role in orchestrating LC migration in the skin. This seems not to be the true for the IL-23/IL-17A pathway.

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“…Its determination is also used in non-neoplastic lesions which are characterized by uncontrollable proliferation of cells, for example in psoriasis [10]. In this disease, excessive proliferation of cells and an improper keratinization process, together with immune disorders, belong to basic pathogenetic mechanisms [11,12]. Epidermal hyperproliferation is connected with intense cell proliferation in the basal layer and too rapid, thus irregular keratinocyte maturation.…”

Section: Introductionmentioning

confidence: 99%

Influence of phototherapy in psoriasis on ki-67 antigen expression: a preliminary study

Jesionek-Kupnicka

Chomiczewska-Skóra²,

Rotsztejn³

2013

pjp

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Antigen Ki-67 is a non-histone nuclear protein, closely connected with cell proliferation. Determining Ki-67 allows for a quick and reliable evaluation of the growth fraction of the studied cell population. It serves as a marker of proliferative activity in neoplasms and other diseases with excessive cell proliferation such as psoriasis. Evaluation of Ki-67 expression in epidermal cells was performed in 10 individuals suffering from plaque psoriasis, before and after one of three methods of phototherapy: broadband UVB, narrowband UVB or PUVA. We observed increased Ki-67 antigen expression in psoriatic lesions. The percentage of Ki-67 positive nuclei in the epidermis affected by the disease ranged from 0 to 30% and in the macroscopically healthy surrounding tissue the percentage was from 0 to 10%. After phototherapy the expression in lesional skin decreased (p < 0.01). The greatest decrease was observed after the application of PUVA therapy (from 25 to 10% of cells, p < 0.05). No significant differences were observed in the perilesional skin with regard to Ki-67 antigen expression before or after phototherapy. On the basis of our own study and studies conducted by other researchers, it can be concluded that reduced Ki-67 expression after the application of PUVA and UVB therapies in the psoriatic epidermis is not a characteristic result of phototherapy only. It is a characteristic property of any effective psoriasis therapy.

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Clinical immunology Density of Langerhans cells in chronic plaque psoriatic lesions before and after phototherapy

Cited by 4 publications

References 33 publications

Climatotherapy at the Dead Sea for psoriasis is a highly effective anti‐inflammatory treatment in the short term: An immunohistochemical study

Climatotherapy at the Dead Sea for psoriasis is a highly effective anti‐inflammatory treatment in the short term: An immunohistochemical study

Langerhans cell markers CD1a and CD207 are the most rapidly responding genes in lesional psoriatic skin following adalimumab treatment

Influence of phototherapy in psoriasis on ki-67 antigen expression: a preliminary study

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