Biological Effect of Organically Coated<i>Grias neuberthii</i>and<i>Persea americana</i>Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines

Salazar, Lizeth; López, María José Vallejo; Grijalva, Marcelo; Castillo, Luis; Maldonado, Alexander

doi:10.1155/2018/9689131

Cited by 12 publications

(3 citation statements)

References 72 publications

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“…Cell viability of three cell lines (HeLa, HepG2, and A549 cells) decreased significantly after incubation with AgNPs at various concentrations, and toxic effects of AgNPs on HeLa cells after 2 h (IC 50 = 7.711) was more pronounced when compared to that on Hep G2 cells (IC 50 = 12.44), while after 24 incubation hours, the values were closer to the three cancer cells studied (IC 50 = 6.72 for Hela cells, IC 50 = 6.88 for Hep G2 cells, and IC 50 = 6.08 for A549 cells). These results proved the high cytotoxic effect of AgNPs on HeLa, HepG2, and A549 cells compared with previous research [ 60 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 ] respectively, and it can be assumed that this higher cytotoxicity originates from phenolic compounds from St John’s extract that coat these nanoparticles. To achieve targeting we are currently working on the functionalization of the AgNPs with cancer-specific aptamers.…”

Section: Resultssupporting

confidence: 81%

Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities

et al. 2021

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This contribution focuses on the green synthesis of silver nanoparticles (AgNPs) with a size < 100 nm for potential medical applications by using silver nitrate solution and Hypericum Perforatum L. (St John’s wort) aqueous extracts. Various synthesis methods were used and compared with regard to their yield and quality of obtained AgNPs. Monodisperse spherical nanoparticles were generated with a size of approximately 20 to 50 nm as elucidated by different techniques (SEM, TEM). XRD measurements showed that metallic silver was formed and the particles possess a face-centered cubic structure (fcc). SEM images and FTIR spectra revealed that the AgNPs are covered by a protective surface layer composed of organic components originating from the plant extract. Ultraviolet-visible spectroscopy, dynamic light scattering, and zeta potential were also measured for biologically synthesized AgNPs. A potential mechanism of reducing silver ions to silver metal and protecting it in the nanoscale form has been proposed based on the obtained results. Moreover, the AgNPs prepared in the present study have been shown to exhibit a high antioxidant activity for 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation, and super oxide anion radical and 2,2-diphenyl-1-picrylhydrazyl. Synthesized AgNPs showed high cytotoxicity by inhibiting cell viability for Hela, Hep G2, and A549 cells.

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Section: Resultssupporting

confidence: 81%

Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities

et al. 2021

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“…Moreover, the cytotoxicity of silver nanoparticles was checked against different cancer cell lines including HepG2 (liver carcinoma), Caco2 (colon carcinoma), MC3T3-C1 (osteoblast) [18], MCF-7 (breast cancer), and HeLa (cervical cancer) cells [20]. Thus, we aimed to investigate the ability of Aspergillus fumigatus to produce silver nanoparticles using varied conditions and resources and further tested their antibacterial and antiproliferative potential using various bacterial strains and liver carcinoma cell line, HepG2, respectively.…”

Section: Introductionmentioning

confidence: 99%

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Shahzad

Saeed

Iqtedar

et al. 2019

Journal of Nanomaterials

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The biogenesis of silver nanoparticles by fungi is an ecologically clean and nontoxic method compared to other physical and chemical methods. Thus, we aimed to discuss the mycosynthesis of extracellular size-controlled AgNPs. After comprehensive screening, Aspergillus fumigatus BTCB10 (KY486782) was selected for the synthesis of AgNPs of controlled size. Characterization was performed by UV-Vis spectrophotometer, Zetasizer, X-Ray Diffraction (XRD), FTIR (Fourier-transform infrared), Atomic Force Microscopy (AFM), and Scanning Electron Microscopy (SEM) along with functional assays—antibacterial and MTT assays. Data suggested that under optimized conditions, i.e., temperature 25°C, AgNO3 concentration 1 mM, biomass 7 g, fungal culture age 7 days, pH 6, ratio of cell-free filtrate (CFF)/silver nitrate (3 : 2), NaCl 20%, and under dark light, the smallest size AgNPs of 0.681 nm with 100% monodispersity was obtained as evident by a zeta potential of -23.4 mV, UV-Vis band at 400 nm, and the presence of O-H and C=O groups confirmed by ATR-FTIR; XRD revealed the crystalline nature of AgNPs; additionally, cube-shaped AgNPs were revealed by Scanning Electron Microscopy (SEM). Moreover, synthesized AgNPs exhibited antibacterial activity against multidrug-resistant bacterial strains, notably, Klebsiella pneumoniae BTCB04, Acinetobacter BTCB05, Pseudomonas aeruginosa BTCB01, and Escherichia coli BTCB03, while maximum 7-fold was observed with Acinetobacter BTCB05. AgNPs demonstrated no cytotoxic activity against HepG2 cells; however, in combination with cisplatin, antiproliferative and cytotoxic effects became more evident and significant in comparison to control and as single agent. Taken together, the data suggested that economical and smallest size AgNPs can be biosynthesized from Aspergillus fumigatus BTCB10 and be used as antibacterial and antiproliferative agents in combination with current drugs against clinically relevant multiple drug-resistant bacterial and tumoral cells. Further studies are required to confirm their effects employing in vivo disease models.

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“…Because of their contact with the bacterial cell wall, 4NBZnO NPs have antibacterial properties. The NPs' activity could be initiated in two ways: by directly or electrostatically interacting with the NPs and causing injury to the bacterial cell wall's outer membrane [53]. Second, producing ROS involves the formation of H 2 O 2 and the release of zinc ions into a bacterium-infested environment, which is toxic [54,55].…”

Section: Discussionmentioning

confidence: 99%

Synthesis and characterization of 4‐nitro benzaldehyde with ZnO‐based nanoparticles for biomedical applications

Indumathi,

Kumaresan,

Suriyaprakash

et al. 2023

J Basic Microbiol

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Globally, cancer is the leading cause of death and morbidity, and skin cancer is the most common cancer diagnosis. Skin problems can be treated with nanoparticles (NPs), particularly with zinc oxide (ZnO) NPs, which have antioxidant, antibacterial, anti‐inflammatory, and anticancer properties. An antibacterial activity of zinc oxide nanoparticles prepared in the presence of 4‐nitrobenzaldehyde (4NB) was also tested in the present study. In addition, the influence of synthesized NPs on cell apoptosis, cell viability, mitochondrial membrane potential (MMP), endogenous reactive oxygen species (ROS) production, apoptosis, and cell adhesion was also examined. The synthesized 4‐nitro benzaldehyde with ZnO (4NBZnO) NPs were confirmed via characterization techniques. 4NBZnO NPs showed superior antibacterial properties against the pathogens tested in antibacterial investigations. As a result of dose‐based treatment with 4NBZnO NPs, cell viability, and MMP activity of melanoma cells (SK‐MEL‐3) cells were suppressed. A dose‐dependent accumulation of ROS was observed in cells exposed to 4NBZnO NPs. As a result of exposure to 4NBZnO NPs in a dose‐dependent manner, viable cells declined and apoptotic cells increased. This indicates that apoptotic cell death was higher. The cell adhesion test revealed that 4NBZnO NPs reduced cell adhesion and may promote apoptosis of cancer cells because of enhanced ROS levels.

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Biological Effect of Organically CoatedGrias neuberthiiandPersea americanaSilver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines

Cited by 12 publications

References 72 publications

Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities

Hypericum perforatum L.-Mediated Green Synthesis of Silver Nanoparticles Exhibiting Antioxidant and Anticancer Activities

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Synthesis and characterization of 4‐nitro benzaldehyde with ZnO‐based nanoparticles for biomedical applications

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