Biological decolorization of azo dyes from textile wastewater effluent by Aspergillus niger

Abstract

I N this study, fungal isolate D2-1 was isolated from contaminated soil collected from textile industry companies and showed high potentiality for decolorization of two different azo dyes. This isolate was identified as Aspergillus niger D2-1 using morphological and cultural characteristic as well ITS gene sequencing. Decolorization process under different optimized condition of azo dye concentration, pH, incubation periods, inoculum size and different carbon and nitrogen sources were investigated. The maximum decolorization efficiency against 100 ppm dye concentration of reactive yellow (4GL) and reactive red (4BL) dyes of 98.62 %and 92.42 %, respectively, were recorded for Aspergillus niger D2-1 at pH 9.0, in presence of 2 % glucose and 0.5% yeast extract as carbon and nitrogen sources respectively at room temperature after 7 days on shaking conditions. The decolorization percentage was confirmed by ultraviolet-visible (UV-Vis) spectrum analysis of untreated/treated reactive yellow (4GL) and reactive red (4BL) dyes, which showed complete disappearance of peaks at ~ 425 nm and at ~ 520 nm, indicates the degradation of dyes due to fungal activity. Treatment of textile wastewater effluent by Aspergillus niger D2-1showed high decolorization percentage (59%) for effluent, also physico-chemical characteristics of textile effluent such as chemical oxygen demand (COD), total dissolved solids(TDS) and total suspended solids (TSS) were decreased from 756 mg/L, 1597 mg/Land 821 mg/L to 391 mg/L, 845 mg/L and 362 mg/L respectively. Moreover, Gas chromatography-mass spectroscopy (GC-MS) of textile effluent before and after treatment were recorded and confirmed the potentiality of Aspergillus niger D2-1 in dyes wastewater treatment.

Fungal endophytes from leaves of Avicennia marina growing in semi‐arid environment as a promising source for bioactive compounds

“…All isolates were described depending on their morphological characteristics according to Khalil et al (2013 ) 2019and Salem et al (2019). Macroscopic morphological features including colour, texture and diameter of colonies and microscopic characteristics including vegetative and reproductive structures of the fungi were noted (Hasanin et al 2020;Hashem et al 2020).…”

Biological Treatment of Real Textile Effluent Using Aspergillus flavus and Fusarium oxysporium and Their Consortium along with the Evaluation of Their Phytotoxicity

“…Approximately, one million tons of dyes are produced every year worldwide, out of which nearly 50% are discharged in the effluent streams or end up in landfills [ 12 ]. The environmental problems correlated with textile effluents occur because of the use of various non-degradable dyes such as azo dyes, high biochemical and chemical oxygen demand (BOD and COD), high pH, salts, and increased levels of suspended solids [ 13 , 14 , 15 ]. Moreover, textile wastewater contains other pollutants, such as color residues, inorganic compounds, catalytic chemicals, dye waste, and cleaning solvents [ 16 , 17 ].…”

“…However, these methods have some restrictions, such as lesser capability, higher costs, and resulting hazardous by-products [ 24 ]. Nowadays, effective biological treatment processes are of great value due to their eco-friendly, low cost, and minor sludge-giving properties [ 15 , 25 , 26 ]. Different metabolites produced by microbes have a high potentiality in various biological activities [ 15 ].…”

Optimization of green biosynthesized visible light active CuO/ZnO nano-photocatalysts for the degradation of organic methylene blue dye

“…The common problems faced by textile industries are related to the residual dyes in the dyeing bath. Such industries implement innovative, rapid, and sustainable treatment methods to enhance the color removal of wastewater [ 4 , 5 ]. Furthermore, legislation and laws related to the limits of discharge and reduction in the removal of colors are increasingly stringent [ 6 ].…”