Fungal endophytes from leaves of
            <i>Avicennia marina</i>
            growing in semi‐arid environment as a promising source for bioactive compounds

Khalil, Ahmed M. A.; Abdelaziz, Amer M.; Khaleil, Mona M.; Hashem, Amr H.

doi:10.1111/lam.13414

Cited by 90 publications

(92 citation statements)

References 66 publications

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“…Biological reactions usually produce ROS as a by-product, causing cell death due to oxidative damage to biological materials [ 42 ]. In this context, similar findings have been reported by many studies that illustrated the antioxidant properties of endophytic fungi, particularly in mangrove plants [ 43 , 44 , 45 ]. The increasing antioxidant activity of A. ochraceus under salt stress is due to the stress that induces the fungus to activate the antioxidant system by producing antioxidant compounds that prevent the damage of ROS [ 46 , 47 ].…”

Section: Discussionsupporting

confidence: 89%

“…In our study, seven fungal endophyte strains were previously isolated from leaves of Avicennia marina growing in a semi-arid environment. The fungal endophytes Aspergillus hiratsukae , Alternaria tenuissima , Chaetomium sp., Curvularia lunata , Chaetomium sp., Chaetomium globosum , and Aspergillus ochraceus were deposited in a gene bank with the accession numbers MT089951, MT089952, MT089953, MT089955, MT089956, MT089957, and MT089958, respectively [ 45 ]. All strains were inoculated on malt extract agar (MEA) (Oxoid) plates, incubated for 3–5 days at 28 ± 2 °C, and then kept at 4 °C for further use [ 103 ].…”

Section: Methodsmentioning

confidence: 99%

“…A. ochraceus was cultured on MEA media containing 0, 15%, and 30% seawater, then incubated for 7 days at 28 ± 2 °C. Crude extracts were assessed for antioxidant activity at different concentrations (1000, 500, 250, 125, 62.5, 31.25, 15.62, and 7.81 µg mL −1 ) according to the DPPH method described by Khalil et al [ 45 ], with minor modifications.…”

Section: Methodsmentioning

confidence: 99%

“…The antifungal activity of crude extracts of A. ochraceus in the case of 0, 15%, and 30% seawater was evaluated against phytopathogenic Fusarium oxysporum RCMB 08213, which was purchased from the Regional Center of Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt. The antifungal activity was evaluated according to the method used by Khalil et al [ 45 ], with modifications. Additionally, the minimum inhibitory concentration (MIC) for each extract was determined using the broth micro-dilution method [ 105 ].…”

Section: Methodsmentioning

confidence: 99%

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Enhancement of Seawater Stress Tolerance in Barley by the Endophytic Fungus Aspergillus ochraceus

et al. 2021

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Symbiotic plant-fungi interaction is a promising approach to alleviate salt stress in plants. Moreover, endophytic fungi are well known to promote the growth of various crop plants. Herein, seven fungal endophytes were screened for salt tolerance; the results revealed that Aspergillus ochraceus showed a great potentiality in terms of salt tolerance, up to 200 g L−1. The indole acetic acid (IAA) production antioxidant capacity and antifungal activity of A. ochraceus were evaluated, in vitro, under two levels of seawater stress, 15 and 30% (v/v; seawater/distilled water). The results illustrated that A. ochraceus could produce about 146 and 176 µg mL−1 IAA in 15 and 30% seawater, respectively. The yield of IAA by A. ochraceus at 30% seawater was significantly higher at all tryptophan concentrations, as compared with that at 15% seawater. Moreover, the antioxidant activity of ethyl acetate extract of A. ochraceus (1000 µg mL−1) at 15 and 30% seawater was 95.83 ± 1.25 and 98.33 ± 0.57%, respectively. Crude extracts of A. ochraceus obtained at 15 and 30% seawater exhibited significant antifungal activity against F. oxysporum, compared to distilled water. The irrigation of barley plants with seawater (15 and 30%) caused notable declines in most morphological indices, pigments, sugars, proteins, and yield characteristics, while increasing the contents of proline, malondialdehyde, and hydrogen peroxide and the activities of antioxidant enzymes. On the other hand, the application of A. ochraceus mitigated the harmful effects of seawater on the growth and physiology of barley plants. Therefore, this study suggests that the endophytic fungus A. ochraceus MT089958 could be applied as a strategy for mitigating the stress imposed by seawater irrigation in barley plants and, therefore, improving crop growth and productivity.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of Seawater Stress Tolerance in Barley by the Endophytic Fungus Aspergillus ochraceus

et al. 2021

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“…Cytotoxicity of mTHPP and mTHPP-loaded nanocomposite was determined in vitro using Vero tissue culture: the MTT protocol [62,63] with minor modification. The Vero (normal fibroblast cell line) was obtained from the American-type culture collection (ATCC).…”

Section: Determination Of Minimal Inhibitory Concentration (Mic) and Minimal Bactericidal Concentration (Mbc)mentioning

confidence: 99%

Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(m-Hydroxyphenyl)porphyrin

et al. 2021

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The development of new antimicrobial strategies that act more efficiently than traditional antibiotics is becoming a necessity to combat multidrug-resistant pathogens. Here we report the efficacy of laser-light-irradiated 5,10,15,20-tetrakis(m-hydroxyphenyl)porphyrin (mTHPP) loaded onto an ethylcellulose (EC)/chitosan (Chs) nanocomposite in eradicating multi-drug resistant Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans. Surface loading of the ethylcelllose/chitosan composite with mTHPP was carried out and the resulting nanocomposite was fully characterized. The results indicate that the prepared nanocomposite incorporates mTHPP inside, and that the composite acquired an overall positive charge. The incorporation of mTHPP into the nanocomposite enhanced the photo- and thermal stability. Different laser wavelengths (458; 476; 488; 515; 635 nm), powers (5–70 mW), and exposure times (15–45 min) were investigated in the antimicrobial photodynamic therapy (aPDT) experiments, with the best inhibition observed using 635 nm with the mTHPP EC/Chs nanocomposite for C. albicans (59 ± 0.21%), P. aeruginosa (71.7 ± 1.72%), and S. aureus (74.2 ± 1.26%) with illumination of only 15 min. Utilization of higher doses (70 mW) for longer periods achieved more eradication of microbial growth.

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Mangroves

Kashyap,

Radhakrishnan,

Singh

et al. 2023

Pharmacological Studies in Natural Oral Care

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Fungal endophytes from leaves of Avicennia marina growing in semi‐arid environment as a promising source for bioactive compounds

Cited by 90 publications

References 66 publications

Enhancement of Seawater Stress Tolerance in Barley by the Endophytic Fungus Aspergillus ochraceus

Enhancement of Seawater Stress Tolerance in Barley by the Endophytic Fungus Aspergillus ochraceus

Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(m-Hydroxyphenyl)porphyrin

Mangroves

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