Biological control of stem rot of groundnut (<i>Arachis hypogaea</i>L.) caused by<i>Sclerotium rolfsii</i>Sacc. with actinomycetes

Adhilakshmi, M.; Latha, P.; Paranidharan, V.; Balachandar, D.; Velazhahan, R.

doi:10.1080/03235408.2013.809224

Cited by 21 publications

(6 citation statements)

References 38 publications

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“…Actinobacterial isolate Actino 48 more effectively inhibited mycelia growth of S. rolfssii than other actinobacterial isolates. These results agree with those obtained by Adhilakshmi et al [39], who reported that Streptomyces sp. MDU most effectively inhibited the growth of S. rolfsii.…”

Section: Discussionsupporting

confidence: 93%

Application of Bio-Friendly Formulations of Chitinase-Producing Streptomyces cellulosae Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by Sclerotium rolfsii

Abo-Zaid

Abdelkhalek

Matar

et al. 2021

JoF

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Of ten actinobacterial isolates, Streptomyces cellulosae Actino 48 exhibited the strongest suppression of Sclerotium rolfsii mycelium growth and the highest chitinase enzyme production (49.2 U L-1 min-1). The interaction between Actino 48 and S. rolfsii was studied by scanning electron microscope (SEM), which revealed many abnormalities, malformations, and injuries of the hypha, with large loss of S. rolfsii mycelia density and mass. Three talc-based formulations with culture broth, cell-free supernatant, and cell pellet suspension of chitinase-producing Actino 48 were characterized using SEM, Fourier transform infrared spectroscopy (FTIR), and a particle size analyzer. All formulations were evaluated as biocontrol agents for reducing damping-off, root rot, and pods rot diseases of peanut caused by S. rolfsii under greenhouse and open-field conditions. The talc-based culture broth formulation was the most effective soil treatment, which decreased the percentage of peanut diseases under greenhouse and open-field conditions during two successive seasons. The culture broth formulation showed the highest increase in the dry weight of peanut shoots, root systems, and yielded pods. The transcriptional levels of three defense-related genes (PR-1, PR-3, and POD) were elevated in the culture broth formulation treatment compared with other formulations. Subsequently, the bio-friendly talc-based culture broth formulation of chitinase-producing Actino 48 could potentially be used as a biocontrol agent for controlling peanut soil-borne diseases caused by S. rolfsii.

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Section: Discussionsupporting

confidence: 93%

Application of Bio-Friendly Formulations of Chitinase-Producing Streptomyces cellulosae Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by Sclerotium rolfsii

Abo-Zaid

Abdelkhalek

Matar

et al. 2021

JoF

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“…46 Bacillus subtilis and Streptomyces cellulosae have been proven to decrease the peanut pod rot disease caused by Fusarium solani and Sclerotium rolfsii. 47,48 Microplastics could act as a reservoir and shelter for soil pathogens, causing a negative effect on beneficial bacteria present in soil and increasing the risk of plant infection by soil-borne diseases. 49,50 In our study, the relative abundance of Bacillus subtilis and Streptomyces cellulosae in the geocarposphere soil was reduced with microplastic contamination, but induced by biochar application.…”

Section: Discussionmentioning

confidence: 99%

“…The increase in some specific taxa of soil bacteria often positively correlated with soil‐borne disease suppression 46 . Bacillus subtilis and Streptomyces cellulosae have been proven to decrease the peanut pod rot disease caused by Fusarium solani and Sclerotium rolfsii 47,48 . Microplastics could act as a reservoir and shelter for soil pathogens, causing a negative effect on beneficial bacteria present in soil and increasing the risk of plant infection by soil‐borne diseases 49,50 .…”

Section: Discussionmentioning

confidence: 99%

Biochar alleviated the toxic effects of microplastics‐contaminated geocarposphere soil on peanut (Arachis hypogaea L.) pod development: roles of pod nutrient metabolism and geocarposphere microbial modulation

Yang,

Liang,

et al. 2023

J Sci Food Agric

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BACKGROUNDThe accumulation of microplastics in agricultural soil poses a threat to the sustainability of agriculture, impacting crop growth and soil health. Due to the geocarpy feature of peanut, geocarposphere soil environment is critical to pod development and its nutritional quality. While the effects of microplastics in the rhizosphere have been studied, their impact on peanut pod in the geocarposphere remains unknown. Biochar has emerged as a potential soil agent with the ability to remediate soil contamination. However, the mechanisms of biochar in mitigating the toxic effects of microplastics‐contaminated geocarposphere soil on peanut pod development remain largely unexplored.RESULTSWe evaluated the peanut pod performance and microbiome when facing microplastics contamination and biochar amendment in geocarposphere soil. The results showed that microplastics present in geocarposphere soil could directly enter the peanut pod, cause pod developmental disorder and exert adverse effects on nutritional quality. Aberrant expression of key genes associated with amino acid metabolism, lipid synthesis, and auxin and ethylene signaling pathways were the underlying molecular mechanisms of microplastics‐induced peanut pod developmental inhibition. However, these expression abnormalities could be reversed by biochar application. In addition, peanut geocarposphere microbiome results showed that biochar application could restore the diversity of microbial communities inhibited by microplastics contamination and promote the relative abundance of bacteria correlated with pathogen resistance and nitrogen cycle of geocarposphere soil, further promoting peanut pod development.CONCLUSIONThis study demonstrated that biochar application is an effective strategy to mitigate the toxic effects of microplastics‐contaminated geocarposphere soil on pod development and nutritional quality. © 2023 Society of Chemical Industry.

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“…The cultivars were grouped into group 1 (resistant), group 2 (moderately resistant), group 3 (susceptible) and group 4 (highly susceptible cultivars) based on percent disease incidence (PDI) in groundnut cultivars. The pathogen namely Sclerotium was isolated from the infected stem regions of groundnut through tissue segmentation method (Adhilakshmi et al, 2014) from Aliyar, Coimbatore, Sivaganga and Madurai region.…”

Section: Survey and Stem Rot Pathogenmentioning

confidence: 99%

Culture-dependent analysis of seed bacterial endophyte, Pseudomonas spp. EGN 1 against the stem rot disease (Sclerotium rolfsii Sacc.) in groundnut

Archana

Rajendran

Manoranjitham

et al. 2020

Egypt J Biol Pest Control

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Background Groundnut, Arachis hypogaea L., crop is an important oil seed crop in India and is prone to attack by numerous fungal and viral diseases, among the soil-borne diseases, stem rot caused by Sclerotium rolfsii Sacc. is economically important. Main body of abstract Incidence (86.6%) of the stem rot disease was recorded in Coimbatore district, Tamil Nadu, India, during the roving survey. For its management, an attempt was made to isolate bacterial endophyte from peanut seeds of different groundnut cultivars by culture-dependent analysis. Totally, 16 bacterial endophytes (endophytes groundnut (EGN) 1 to EGN 16 along with standards TNAU-Pf1 and EPC 5) were obtained and characterized through morphological, biochemical and molecular studies and also phytostimulation activities were performed. Among the isolates, EGN 1 and EGN 4 showed positive results for indole acetic acid (IAA), siderophore, phosphate solubilization and protease tests in vitro. The dual culture analysis showed inhibition rates of 60.1% (dual plate assay), 68.23% (filter paper disc assay) and 100% (triangle method of streaking and culture filtrate assay) for EGN 1 against S. rolfsii. Further, the crude metabolite assay showed 97.7% inhibition in EGN 1, followed by 87.7% in EGN 4. The roll towel study showed a high vigour index of 4286.7 in EGN 1; hence, this isolate was chosen. Further, thin-layer chromatography (TLC) analysis showed various bands at 0.72 Rf, whereas GC-MS analysis indicated the prominent peaks of hexadecanoic acid and cis-vaccenic acid that may responsible for antifungal activity. In a molecular approach, the genomic DNA of EGN 1 strain was used to amplify a 1200 bp PCR-fragment and sequenced. Short conclusion The overall outcome of this study showed that Pseudomonas spp. EGN 1 had a great potential as a bio-stimulant and biocontrol agent to manage effectively the stem rot in peanut.

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Biological control of stem rot of groundnut (Arachis hypogaeaL.) caused bySclerotium rolfsiiSacc. with actinomycetes

Cited by 21 publications

References 38 publications

Application of Bio-Friendly Formulations of Chitinase-Producing Streptomyces cellulosae Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by Sclerotium rolfsii

Application of Bio-Friendly Formulations of Chitinase-Producing Streptomyces cellulosae Actino 48 for Controlling Peanut Soil-Borne Diseases Caused by Sclerotium rolfsii

Biochar alleviated the toxic effects of microplastics‐contaminated geocarposphere soil on peanut (Arachis hypogaea L.) pod development: roles of pod nutrient metabolism and geocarposphere microbial modulation

Culture-dependent analysis of seed bacterial endophyte, Pseudomonas spp. EGN 1 against the stem rot disease (Sclerotium rolfsii Sacc.) in groundnut

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