Biological control of root knot nematode, Meloidogyne incognita, in vitro, greenhouse and field in cucumber

Naz, Ishrat; Khan, Raja Asad Ali; Masood, Tariq; Baig, Ayesha; Siddique, Ifra; Haq, Shamsul

doi:10.1016/j.biocontrol.2020.104429

Cited by 31 publications

(14 citation statements)

References 38 publications

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“…Recently Naz et al (2021), also reported that the culture filtrate of one A. flavus strain had both eggs hatching inhibitory activity and J2s mortality of M. incoginta. Compared to J2s mortality between the two A. flavus strains, JCK-4087 showed much stronger nematicidal activity than the A. flavus strain reported (Naz et al, 2021) with J2s mortality values of 100% at a concentration of 20% for the former and about 40% at a concentration of 25% for the latter. Both A. flavus JCK-4087 and P. commune KACC 45973 were found to produce CPA as a nematicidal metabolite.…”

Section: Discussionmentioning

confidence: 95%

“…In our present study, the culture filtrate of A. flavus JCK-4087 showed strong nematicidal activity against M. incognita . Recently Naz et al (2021) , also reported that the culture filtrate of one A. flavus strain had both eggs hatching inhibitory activity and J2s mortality of M. incoginta . Compared to J2s mortality between the two A. flavus strains, JCK-4087 showed much stronger nematicidal activity than the A. flavus strain reported ( Naz et al, 2021 ) with J2s mortality values of 100% at a concentration of 20% for the former and about 40% at a concentration of 25% for the latter.…”

Section: Discussionmentioning

confidence: 95%

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Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process

Nguyen

Lee

et al. 2021

Front. Microbiol.

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Among 200 fungal strains isolated from the soil, only one culture filtrate of Aspergillus flavus JCK-4087 showed strong nematicidal activity against Meloidogyne incognita. The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JCK-4087 also produced aflatoxins, six strains of Penicillium commune, which have been reported to be CPA producers, were obtained from the bank and then tested for their CPA productivity. CPA was isolated from the culture filtrate of P. commune KACC 45973. CPA killed the second-stage juveniles of M. incognita, M. hapla, and M. arearia with EC50–3 days 4.50, 18.82, and 60.51 μg mL–1, respectively. CPA also significantly inhibited egg hatch of M. incognita and M. hapla after a total of 28 days of treatment with the concentrations > 25 μg mL–1. The enhancement of CPA production by P. commune KACC 45973 was explored using an optimized medium based on Plackett–Burman design (PBD) and central composite design (CCD). The highest CPA production (381.48 μg mL–1) was obtained from the optimized medium, exhibiting an increase of 7.88 times when compared with that from potato dextrose broth culture. Application of the wettable power-type formulation of the ethyl acetate extract of the culture filtrate of KACC 45973 reduced gall formation and nematode populations in tomato roots and soils under greenhouse conditions. These results suggest that CPA produced by P. commune KACC 45973 can be used as either a biochemical nematicide or a lead molecule for developing chemical nematicides to control root-knot nematodes.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 95%

Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process

Nguyen

Lee

et al. 2021

Front. Microbiol.

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“…The selected nematophagous fungus colonizes on the brinjal plant and proliferates in the rhizosphere of brinjal. In crop plants, the successful biocontrol of nematodes is mainly based on the establishment, proliferation, and survival of fungi through soil as spores capable of attacking nematodes [ 35 ]. The present result revealed increased colonization after inoculums of fungal spores.…”

Section: Discussionmentioning

confidence: 99%

Paecilomyces formosus MD12, a Biocontrol Agent to Treat Meloidogyne incognita on Brinjal in Green House

Baazeem

Alorabi

Palanisamy

et al. 2021

JoF

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The present study was carried out to analyze the potential of fungi isolated from the rhizosphere of soybean, brinjal, tomato, and potato plants. The density of fungi varied in the pot soil and rhizosphere after Paecilomyces formosus MD12 treatment. The P. formosus MD12 population was 6.3 ± 0.13 × 104 CFU g−1 in the pot planted with brinjal, and the population increased in the rhizosphere (6.72 ± 0.41 × 104 CFU g−1). P. formosus MD12 was cultured in the production medium, and the supernatant was used for egg inhibition studies on a root-knot nematode parasite, Meloidogyne incognita. It was revealed that maximum egg inhibition (94.7 ± 6.2%) was obtained at 100% concentration of extract. The culture supernatant from P. formosus MD12 affected the development of M. incognita juvenile, and the mortality rate was maximum after 96 h (95 ± 6%). Mortality was reduced when treated with 25%, 50%, and 75% supernatant. At 1 × 107 mL−1 of spore suspension, we found reductions of 71.6 ± 3.3% nematode populations in the soil, 60.7 ± 2.2% from the root, and 63.6 ± 2.4% egg mass compared with the control in the pot experiment. The culture supernatant applied at the 10% level showed a maximum mean reduction of the nematode population in roots (72.4 ± 2.2%), soil (77.9 ± 2.5%), and egg masses (73.2 ± 1.5%), respectively. The presence of P. formosus MD12 in a soil environment could antagonize nematode parasites and improve soil amendment. The P. formosus MD12 strain showed good biocontrol ability against the root-knot nematode, M. incognita, under in vitro and green house experimental condition.

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“…Among the advances employed to control RKN include crop rotation, application of wide spectrum of chemical nematicides plus use of resistance genes (Mitkowski and Abawi, 2003), use of bioagents (Naz et al, 2020), grafting susceptible varieties onto nematode-resistant rootstocks (Sim and Rosmah, 2011), besides others. Each of the above advances has associated challenges that limit its sustainability; the broad host spectrum of RKN species limits the use of crop rotation (Mitkowski and Abawi, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Each of the above advances has associated challenges that limit its sustainability; the broad host spectrum of RKN species limits the use of crop rotation (Mitkowski and Abawi, 2003). Fumigants and nematicides pose a threat to both humans and the ecosystem due to their gradual environmental toxicities (Naz et al, 2020). Efforts to graft P. nigrum shoots onto P. colubrinum rootstocks yielded high initial success but graft incompatibility (Sim and Rosmah, 2011) led to poor plant survival (Lau et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Reducing root galling caused by root knot nematodes in black pepper (<em>Piper nigrum</em> L. cv. ‘Kuching’) through nutrient supplementation

et al. 2022

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Purpose: Root-knot nematodes (RKN) remain one of the leading causes of economic damage in black pepper (Piper nigrum L.) cultivation worldwide. An experiment was conducted to evaluate the response of black pepper plants to RKN when irrigated with three nutrient solutions; Hoagland solution only, Hoagland solution + 0.005 mM potassium silicate solution, and Hoagland solution + 2 mM salicylic acid solution.Research Method: Plants grown in medium-sized polybags were inoculated with RKN and routinely irrigated with the appropriate nutrient solutions. Presence of foliar symptoms of RKN infection on plants, their aerial growth plus root infestation were assessed. Superoxide anion and hydrogen peroxide production from the plant leaves were also detected after inoculation.Findings: Foliar symptoms of RKN infection started manifesting after 81 days from inoculation. Plants without silicate or salicylic acid supplementation displayed the most severe foliar disorder symptoms, highest root galling and the shortest aerial growth. Plants irrigated with potassium silicate had the highest aerial growth and their leaves showed the least oxidative stress when challenged with RKN.Originality/value: From present results, soil amendment with potassium silicate is a promising tool for reducing root galling and promoting aerial growth in RKN-infested black pepper fields.

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Biological control of root knot nematode, Meloidogyne incognita, in vitro, greenhouse and field in cucumber

Cited by 31 publications

References 38 publications

Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process

Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process

Paecilomyces formosus MD12, a Biocontrol Agent to Treat Meloidogyne incognita on Brinjal in Green House

Reducing root galling caused by root knot nematodes in black pepper (<em>Piper nigrum</em> L. cv. ‘Kuching’) through nutrient supplementation

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