Biological control of rice brown spot with native isolates of three Trichoderma species

Khalili, Elham; Sadravi, Mehdi; Naeimi, Shahram; Khosravi, Vahid

doi:10.1590/s1517-83822012000100035

Cited by 57 publications

(39 citation statements)

References 9 publications

(12 reference statements)

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“…Volatile and non-volatile compounds were the main forms of controlling R. solani by the Trichoderma populations tested, what is in agreement with Khalili et al (2012). The populations ASP24 and the commercial strain also reduced the growth of the pathogen in the dual culture test (Table 1).…”

Section: Resultssupporting

confidence: 82%

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Native Trichoderma grown on oat grains controls damping-off and enhances height in soybean

Roese

Vidal

Zielinski

et al. 2017

Pesqui. Agropecu. Trop.

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Trichoderma is a biological control agent used to improve the resistance to diseases, which may also estimulate plant growth. Commercial products with Trichoderma are available in different countries, but most of them are based on conidial suspension. This study aimed at evaluating the efficiency of native Trichoderma populations collected from different production systems and applied to the soil by using two methods: conidial suspension and inoculated oat grains. The efficiency of native Trichoderma populations collected from conventional crop and agropastoral and agrosilvopastoral systems in a long-term field experiment was evaluated. The populations efficiencies were evaluated by in vivo tests that assessed the control of soybean damping-off caused by Rhizoctonia solani, plant height and soil colonization with the antagonist. In vitro tests, such as dual culture and assessment of volatile and non-volatile compounds, were conducted to study the mode of action of the populations. Some native Trichoderma populations were as efficient as those from a commercial product in all tests. Compared to conidial suspension, Trichoderma spp. inoculated through oat grains promoted a greater damping-off control, higher plants and more colony-forming units per gram of soil after 3 months of application. Native populations performed equally well or even better than the commercial strain, and the use of a substrate that supports the Trichoderma growth was more efficient than the conidial suspension method.

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Section: Resultssupporting

confidence: 82%

“…It may also stimulate plant growth and improve plant resistance to diseases (Verma et al 2007, Lorito et al 2010. It is recovered from soils around the world and from different production systems (Lorito et al 2010, Khalili et al 2012. Trichoderma is also…”

Section: Introductionmentioning

confidence: 99%

Native Trichoderma grown on oat grains controls damping-off and enhances height in soybean

Roese

Vidal

Zielinski

et al. 2017

Pesqui. Agropecu. Trop.

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“…The evaluation of NVMs produced by fungi under laboratory conditions, as well as dual culture, is an initial step in the prospection of metabolites for both biological control and other purposes and can not be overlooked. It can be performed by the technique of cellophane paper (Khalili et al 2012, Jeyaseelan et al 2014, Barari 2016 or also from the liquid culture, filtration and incorporation in the substrate method. Numerous scientific studies have used the methodology of incorporation of filtrates into culture medium for the evaluation of antagonism in different plant pathogenic fungi species, such as Verticillium dahliae Kleb.…”

Section: Introductionmentioning

confidence: 99%

Antifungal potential of crude extracts of Trichoderma spp.

2018

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Antibiosis is the mechanism by which certain microorganisms respond to the presence of others, secreting compounds or metabolites capable of inhibiting or impeding their development. The crude extract of Trichoderma contains a mixture of secondary compounds, which may show antibiotic effect, and has been used for the prospect of this fungus for biological control and other industrial purposes. Faced with the increasing demand of agriculture for ecologically compatible alternatives for the management of diseases, this work aimed to investigate the spectrum of action of NonVolatile Metabolites (NVMs) of Trichoderma isolates against different plant pathogenic fungi. The antagonistic potential of NVMs was evaluated through the incorporation method of the filtered liquid extract in PDA medium. The assays showed that all the NVMs produced inhibited the fungus Sclerotinia sclerotiorum similarly. On the other hand, strains CEN1245 and CEN1274, both belonging to the species Trichoderma brevicompactum, showed broad spectrum against Sclerotium rolfsii, Colletotrichum gloesporioides, Verticillium dahliae, Fusarium oxysporum and Cylindrocladium sp. The present study describes isolates producing non-volatile metabolites with broad spectrum of antifungal action, as well as pathogen-specific. The Trichoderma spp. NVMs obtained from different soil samples cultivated with vegetables, cassava and maize were efficient in inhibiting plant pathogenic fungi belonging to other patossystems, such as forest or fruit, which could increase their potential application in biological control of plant diseases. In addition, these antagonistic fungi should be studied in greater depth for the identification of bioactive molecules of industrial interest or in commercial formulations of products for biological control of plant pathogens.Keywords: secondary metabolites, antagonism, inhibition of mycelial growth, plant pathogenic fungi. Potencial antifúngico de extratos brutos de Trichoderma spp.Resumo: Antibiose é um mecanismo pelo qual certos microrganismos respondem à presença de outros, secretando compostos ou metabólitos capazes de inibir ou impedir o seu desenvolvimento. O extrato bruto de Trichoderma contém uma mistura de compostos secundários e tem sido utilizado na prospecção deste fungo para o controle biológico e demais fins industriais. Diante da crescente demanda da agricultura por alternativas ecologicamente compatíveis para o manejo de doenças, este trabalho teve como objetivo investigar o espectro de ação de Metabólitos Não Voláteis (MNVs), produzidos por isolados de Trichoderma, contra diferentes fungos fitopatogênicos. O potencial antagônico dos MNVs foi avaliado através do método de incorporação do extrato líquido filtrado em meio BDA. Os ensaios mostraram que todos os MNVs produzidos inibiram de forma semelhante o fungo Sclerotinia sclerotiorum. Por outro lado, os isolados CEN1245 e CEN1274, ambos Trichoderma brevicompactum, mostraram um amplo espectro de ação, atuando contra Sclerotium rolfsii, Colletotrichum gloespori...

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“…(Ajith & Lakshmidevi, 2010). T. harzianum juga mampu menghambat Bipolaris oryzae sebesar 72,4% (Khalil, Sadravi, Naeimi, & Khosravi, 2012), sedangkan penelitian lainnya melaporkan bahwa T. harzianum, T. virens, dan T. atroviride dapat menghambat Fusarium moniliforme sebesar 26,27%-32,86% (Kumar, Misra, Modi, & Gupta, 2012).…”

Section: Pengaruh Metabolit Volatil Dan Non Volatil Jamur Antagonis Tunclassified

Evaluasi Jamur Antagonis dalam Menghambat Pertumbuhan Rigidoporus microporus Penyebab Penyakit Jamur Akar Putih pada Tanaman Karet

Amaria

Harni

Samsudin

2015

JTIDP

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ABSTRAKRigidoporus microporus merupakan patogen penyebab penyakit jamur akar putih (JAP) pada karet yang sangat sulit untuk dikendalikan. Penggunaan jamur antagonis diharapkan mampu mengendalikan patogen tersebut karena memiliki kemampuan penghambatan melalui mekanisme kompetisi, antibiosis, atau parasitisme. Penelitian ini bertujuan mengevaluasi 10 isolat jamur antagonis dalam menghambat R. microporus penyebab penyakit JAP pada tanaman karet serta mengidentifikasi mekanisme penghambatannya secara in vitro. Penelitian dilaksanakan bulan Januari sampai April 2013, di Laboratorium Proteksi Tanaman, Balai Penelitian Tanaman Industri dan Penyegar (Balittri) Sukabumi. Penelitian terdiri dari: (1) uji kecepatan pertumbuhan koloni, (2) uji daya hambat, (3) uji metabolit sekunder, dan (4) (3) antagonistic mechanism. Each test using a completely randomized design. The antagonist fungi used are: Trichoderma virens, T. hamatum, T. amazonicum, Eupenicillium javanicum, Penicillium simplicissimum, P. citrinum, P. pinophilum, Paecilomyces lilacinus, Aspergillus fijiensis, and Hypocrea atroviridis (=T. atroviride), in which each of these fungi repeated 3 times. The results showed that the Trichoderma genus have inhibitory capability against R. microporus stronger than the others. Four genera of Trichoderma fungi have better competition mechanism against R. microporus compared to Penicillium, Eupenicillium, Paecilomyces, and Aspergillus. In addition, T. virens and H. atroviridis also have parasitism ability, while P. lilacinus and E. javanicum have antibiosis mechanism against R. microporus. The antagonistic fungi which were most potential to be developed as a biological control for white root disease, i.e.: T. virens, T. hamatum, and H. atroviridis. ABSTRACT Rigidoporus microporus is a pathogen causing white root disease in rubber plants, which is very difficult to control. The use of antagonistic fungi is expected to control this pathogen because it has the inhibitory ability through the competition, antibiosis or parasitism mechanisms. The objective of this research was to evaluate ten antagonistic fungi in inhibiting the growth of R. microporus in vitro. The research was conducted from January to April 2013, in the Laboratory of Plant Protection, Indonesian Industrial Beverages Crop Research Institute (IIBCRI) Sukabumi. The research consisted of: (1) the growth rate of colony, (2) inhibition, (3) secondary metabolites, and

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Biological control of rice brown spot with native isolates of three Trichoderma species

Cited by 57 publications

References 9 publications

Native Trichoderma grown on oat grains controls damping-off and enhances height in soybean

Native Trichoderma grown on oat grains controls damping-off and enhances height in soybean

Antifungal potential of crude extracts of Trichoderma spp.

Evaluasi Jamur Antagonis dalam Menghambat Pertumbuhan Rigidoporus microporus Penyebab Penyakit Jamur Akar Putih pada Tanaman Karet

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