Biological Control of Fusarium oxysporum f.sp. lycopersici on Tomato by Brevibacillus brevis

Abstract

The ability of Brevibacillus brevis to influence development of disease on tomato caused by Fusarium oxysporum f.sp. lycopersici was investigated using plants raised in Petri dish microcosms and in pots in the glasshouse. Development of symptoms on both microcosm-and glasshouse-raised tomato plants was markedly reduced in co-inoculations of F. oxysporum f.sp. lycopersici with B. brevis, compared with inoculations with the pathogen alone. Moreover, co-inoculations resulted in significant growth boosting effects on the plants, with increases in plant height in microcosms and in total root lengths in glasshouse-raised plants. In microcosm-raised plants, the carrier used to inoculate seed with B. brevis, either carboxymethyl cellulose (CMC) or vermiculite, had no effect on the persistence of the biological control agent on roots in the absence of inoculation with the pathogen. By contrast, numbers of B. brevis recovered from the rhizosphere and rhizoplane of inoculated plants in microcosms were four orders of magnitude lower than in plants treated with B. brevis alone. Moreover, higher numbers of B. brevis CFU were re-isolated from the rhizosphere of plants arising from CMC-coated seed, than vermiculite-coated seed. The carrier had no effect on disease control. Inhibition of conidial germination and germtube extension of F. oxysporum f.sp. lycopersici by cellfree filtrates of B. brevis cultures varied significantly depending on the culture medium used for suspension. These results indicate that B. brevis is a potential biological control agent for reducing the impact of F. oxysporum f.sp. lycopersici on tomato.

PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

“…Brevibacillus laterosporus, a Gram-positive, aerobic, sporeforming bacterium previously classified as Bacillus laterosporus, can produce diverse metabolites with antifungal activity, which can control the infection of plant pathogens as biocontrol agents (34). We previously screened a novel strain, A60, that was isolated from the soil of mango plants in Changjiang, Hainan Province, China (19°15.635=N, 108°46.029=E), which was identified as B. laterosporus by phenotypic characterization and 16S rRNA sequencing (35).…”

Biocontrol of Lasiodiplodia theobromae, which causes black spot disease of harvested wax apple fruit, using a strain of Brevibacillus brevis FJAT-0809-GLX

“…brevis is capable of producing different antibiotics that are responsible for disease suppression (Haggag, 2008;Chandel et al, 2010). This species produces a single cyclic decapeptide antibiotic, gramicidin S, which is fungicidal (Murray et al, 1986;Schmitt et al, 1998;Edwards and Seddon, 2001).…”

Controle biológico da murcha do tomateiro causada por Ralstonia solanacearum e Fusarium oxysporum f. sp. lycopersici por rizobactérias

“…Rizobactérias podem estimular os mecanismos de defesa das plantas (Van Loon et al, 1998) ou ter ação direta contra FOL (Chandel et al, 2010). Pesquisadores reconhecem que a produção de quitinases é mecanismo importante na inibição de FOL (Hariprasad et al, 2011).…”