PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

Wang, Haoqian; Yang, Xiufen; Guo, Lan-Ping; Zeng, Hongmei; Qiu, Dewen

doi:10.1128/aem.03586-14

Cited by 52 publications

(49 citation statements)

References 64 publications

(55 reference statements)

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“…Histochemical staining of H 2 O 2 was conducted using 3,3-diaminobenzidine (DAB) as a substrate according to Wang et al (29). Staining of superoxide anions was conducted using the NBT staining method according to Montiel et al (30).…”

Section: Methodsmentioning

confidence: 99%

Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea

Zhou

Yuan

et al. 2016

Appl Environ Microbiol

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bOxygenous terpenoids are active components of many medicinal plants. However, current studies that have focused on enzymatic oxidation reactions cannot comprehensively clarify the mechanisms of oxygenous terpenoid synthesis and diversity. This study shows that an endophytic bacterium can trigger the generation of reactive oxygen species (ROS) that directly increase oxygenous sesquiterpenoid content and diversity in Atractylodes lancea. A. lancea is a famous but endangered Chinese medicinal plant that contains abundant oxygenous sesquiterpenoids. Geo-authentic A. lancea produces a wider range and a greater abundance of oxygenous sesquiterpenoids than the cultivated herb. Our previous studies have shown the mechanisms behind endophytic promotion of the production of sesquiterpenoid hydrocarbon scaffolds; however, how endophytes promote the formation of oxygenous sesquiterpenoids and their diversity is unclear. After colonization by Pseudomonas fluorescens ALEB7B, oxidative burst and oxygenous sesquiterpenoid accumulation in A. lancea occur synchronously. Treatment with exogenous hydrogen peroxide (H 2 O 2 ) or singlet oxygen induces oxidative burst and promotes oxygenous sesquiterpenoid accumulation in planta. Conversely, pretreatment of plantlets with the ROS scavenger ascorbic acid significantly inhibits the oxidative burst and oxygenous sesquiterpenoid accumulation induced by P. fluorescens ALEB7B. Further in vitro oxidation experiments show that several oxygenous sesquiterpenoids can be obtained from direct oxidation caused by H 2 O 2 or singlet oxygen. In summary, this study demonstrates that endophytic bacterium-triggered ROS can directly oxidize oxygen-free sesquiterpenoids and increase the oxygenous sesquiterpenoid content and diversity in A. lancea, providing a novel explanation of the mechanisms of oxygenous terpenoid synthesis in planta and an essential complementarity to enzymatic oxidation reactions.A tractylodes lancea is a traditional Chinese medicinal plant and is the main ingredient of many famous Chinese medicines. Oxygenous sesquiterpenoids, such as hinesol, ␤-eudesmol, atractylone, and caryophyllene oxide, are the main active components in A. lancea and have medicinal efficacy against rheumatic diseases, digestive disorders, night blindness, and influenza (1). The quality of A. lancea strongly depends on the area in which the herb is produced, and A. lancea grown in the Maoshan area of the Jiangsu Province is the geo-authentic herb (2), which is characterized by higher oxygenous sesquiterpenoid content and diversity than herbs grown in other areas (3). In recent years, the geoauthentic A. lancea has become endangered due to habitat destruction and overexploitation. Although cultivation ensures the production of medicinal materials, the oxygenous sesquiterpenoid content and diversity in cultivated A. lancea is considerably decreased compared to that of the wild herb. Currently, knowledge on the biosynthesis and diversity of oxygenous sesquiterpenoids is insufficient. Some studies have explained...

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Section: Methodsmentioning

confidence: 99%

Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea

Zhou

Yuan

et al. 2016

Appl Environ Microbiol

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“…These excitors are derived from a wide variety of sources and types, including proteins, peptides, oligosaccharides and lipids. The elicitors which induce systemic resistance in plants are generally protein peptides and antibiotics in biocontrol bacteria (Wang, Yang, Guo, Zeng, & Qiu, ). For example, lipopeptides and amylin in Bacillus subtilis S499 are involved in the ISR phenomenon that induces disease resistance (Ongena et al, ).…”

Section: Discussionmentioning

confidence: 99%

Screening and biological function of protein elicitor secreted from biocontrol agent Hhs.015

Xia

et al. 2019

Journal of Phytopathology

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Strain Hhs.015, a PGPR strain well‐studied for biocontrol of apple Valsa canker, was isolated from cucumber roots and classified as a new species of Saccharothrix yanglingensis. Hhs.015 was able to colonize apple tissue culture seedlings and trigger disease resistance. According to the whole‐genome sequence of S. yanglingensis Hhs.015, its secreted proteins were predicted and analysed, which can help to find out Hhs.015 functional substances that are involved in inducing plant system resistance and also the molecular mechanisms of the interaction between Hhs.015 and the host plants. Using the software SignalP4.1, TMHMM2.0, DAS‐TMfilter, HMMTOP, ScanProsite, PSORT and big‐PI predictor, 7,379 ORFs of the genome of S. yanglingensis Hhs.015 were predicted and analysed. Moreover, the function of ORFs where the signal peptides come from was predicted by Swissprot database. The selected proteins were obtained by prokaryotic expression. Their effects in inducing plant resistance to the pathogen Valsa mali, resistance‐related enzyme activity and growth promotion analysis were evaluated. In total, 158 ORFs with known function, such as serine proteinase and transpeptidase, were identified; the remaining 142 ORFs were function‐unknown putative proteins. Five putative proteins were successfully obtained by prokaryotic expression, and the results showed that the protein named 5620g could enhance the plant disease resistance to the pathogen V. mali, inhibit the expansion of lesions and upregulate the defence‐related enzyme activity. The protein named 3176g could prolongate cucumber root. In conclusion, biocontrol strain Hhs.015 may secrete protein elicitors to improve plant resistance to the pathogenic microorganism and promote the growth of host plants.

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“…Extracellular peptides were precipitated using the ammonium sulfate precipitation method with slight modifications [35]. Bacillus subtilis was grown in 200 mL liquid LB supplemented with kanamycin (10 mg/L) and incubated at 180 r/min, 37 • C for 72 h. Supernatants were collected by centrifugation at 10,000× g, 4 • C for 20 min.…”

Section: Expression Of Crude Proteinsmentioning

confidence: 99%

“…IiR515 and IiR915 were purified using nickel column affinity chromatography and their antimicrobial activities were confirmed. The fermentation process was carried out as described previously [35]. Briefly, supernatants were collected by centrifugation at 10,000× g and 4 • C for 20 min.…”

Section: Purification Of Extracellular Peptidesmentioning

confidence: 99%

Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System

Abbas

et al. 2019

Biomolecules

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The situation of drug resistance has become more complicated due to the scarcity of plant resistance genes, and overcoming this challenge is imperative. Isatis indigotica has been used for the treatment of wounds, viral infections, and inflammation for centuries. Antimicrobial peptides (AMPs) are found in all classes of life ranging from prokaryotes to eukaryotes. To identify AMPs, I. indigotica was explored using a novel, sensitive, and high-throughput Bacillus subtilis screening system. We found that IiR515 and IiR915 exhibited significant antimicrobial activities against a variety of bacterial (Xanthomonas oryzae, Ralstonia solanacearum, Clavibacter michiganensis, and C. fangii) and fungal (Phytophthora capsici and Botrytis cinerea) pathogens. Scanning electron microscope and cytometric analysis revealed the possible mechanism of these peptides, which was to target and disrupt the bacterial cell membrane. This model was also supported by membrane fluidity and electrical potential analyses. Hemolytic activity assays revealed that these peptides may act as a potential source for clinical medicine development. In conclusion, the plant-derived novel AMPs IiR515 and IiR915 are effective biocontrol agents and can be used as raw materials in the drug discovery field.

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PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

Cited by 52 publications

References 64 publications

Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea

Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea

Screening and biological function of protein elicitor secreted from biocontrol agent Hhs.015

Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System

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