Biological Control of Fusarium Wilt of Strawberry by Nonpathogenic Fusarium oxysporum Isolated from Strawberry.

Tezuka, Nobuo; Makino, Takahiro

doi:10.3186/jjphytopath.57.506

Cited by 24 publications

(12 citation statements)

References 4 publications

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“…Nonpathogenic Fusarium oxysporum is considered as a biocontrol fungus that controls Fusarium wilt by promoting the positive responses of plants, including pepper (Veloso & Díaz, ), cowpea (Vieira et al ., ), banana (Forsyth et al ., ), basil (Larkin & Fravel, ), cucumber (Mandeel & Baker, ), cyclamen (Minuto et al ., ), flax (Alabouvette et al ., ), tomato (Fuchs et al ., ; Olivain et al ., ), spinach (Katzube et al ., ; strawberry (Tezuka & Makino, ), wheat (Pritsch et al ., ) and watermelon (Freeman et al ., ). Previous studies of F. oxysporum have primarily focused on the mechanisms and analyses of the direct and indirect antagonism mainly.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenicFusarium oxysporumCS-20

Xie

et al. 2014

FEMS Microbiol Lett

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A defence response can be induced by nonpathogenic Fusarium oxysporum CS-20 in several crops, but the molecular mechanism has not been clearly demonstrated. In the present study, we analysed the defence mechanism of a susceptible cucumber cultivar (Cucumis sativus L. 9930) against a pathogen (F. oxysporum f. sp. cucumerinum) through the root precolonization of CS-20. A challenge inoculation assay indicated that the disease severity index (DSI) was reduced, ranging from 18.83 to 61.67 in comparison with the pathogen control. Root colonization analysis indicated that CS-20 clearly did not appear to influence the growth of cucumber seedlings. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) revealed that CS-20-mediated defence response was activated by PR3, LOX1 and PAL1 and the pathogenmediated resistance response was regulated by PR1 and PR3. Moreover, both nonpathogenic and pathogenic F. oxysporum were able to upregulate NPR1 expression. In contrast to a pathogen, CS-20 can activate the Ca 2+ /CaM signal transduction pathway, and the gene expression of both CsCam7 and CsCam12 increased significantly. The gene expression analysis indicated that CS-20 strongly enhanced the expression of PR3, LOX1, PAL1, NPR1, CsCam7 and CsCam12 after inoculation. Overall, the defence response induced by CS-20 can be controlled by multiple genes in the cucumber plant.

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Section: Introductionmentioning

confidence: 99%

Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenicFusarium oxysporumCS-20

Xie

et al. 2014

FEMS Microbiol Lett

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“…Nonpathogenic F. oxysporum has often been introduced to disinfested soils as biocontrol agents to suppress pathogenic F. oxysporum (e.g., Tezuka and Makino 1991;Ichikawa et al 1998). In such experiments, it was possible to investigate population dynamics of both pathogenic and nonpathogenic forms when these media were combined with Fo-N1 or Fo-N2 (Nishimura 2002).…”

Section: Discussionmentioning

confidence: 99%

Selective media for Fusarium oxysporum

Nishimura

2007

J Gen Plant Pathol

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Selective media without pentachloronitrobenzene were developed for quantitative assays of Fusarium oxysporum in soils. Media Fo-G1 and Fo-G2 were effective for naturally infested soils, Fo-W1 and Fo-W2 for wildtype isolates in soils containing a nitrate-nonutilizing (nit) mutant, and Fo-N1 and Fo-N2 for nit mutants. Selective media were made using ammonium citrate dibasic, L-sorbose, econazole nitrate, 25% iminoctadine triacetate solution and 50% tolclofos-methyl wettable powder for soil dilutions of 100-fold or more (Fo-G1, FoW1 and Fo-N1) and 10-fold (Fo-G2, Fo-W2 and Fo-N2

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“…The isolation frequency was higher than the rate of aerial hypha formation 3 days after SDEI but was equivalent after 10 days. Tezuka and Makino (1991) isolated nonpathogenic F. oxysporum from the crown of asymptomatic strawberry plants and used it as a biological control agent. In the present study, all isolates caused Fusarium wilt.…”

Section: Discussionmentioning

confidence: 99%

Simple diagnosis using ethanol immersion of strawberry plants with latent infection by Colletotrichum acutatum, Dendrophoma obscurans, and Fusarium oxysporum f. sp. fragariae

Ishikawa

2004

J Gen Plant Pathol

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Simple diagnosis by ethanol immersion (SDEI) to detect Glomerella cingulata was used to detect three other fungi that also cause latent infection of strawberry plants. Signs on strawberry leaves with asymptomatic latent infection by Colletotrichum acutatum became visible using SDEI. Salmon-pink conidial masses were produced in the acervuli on the treated leaves 5 days after incubation at 28°C. In the case of Dendrophoma obscurans, pycnidia with amber conidial masses formed 5 days after incubation at 28°C. The pycnidia were observed mainly on the ribs, and conidial masses exuded from the ostiole. These macroscopic conidial masses were similar to those of G. cingulata and C. acutatum. When water was dripped onto a lesion caused by D. obscurans, the pycnidia exuded white filamentous conidial masses, making the distinction of D. obscurans from G. cingulata or C. acutatum. On petioles with latent infection by Fusarium oxysporum f. sp. fragariae, white aerial hyphae grew out from the vascular tissues on the cut surface 3 days after incubation at 28°C and were easily observed by eye or with a loupe. Thus, SDEI was also useful for diagnosing latent infection of strawberry plants by C. acutatum, D. obscurans, and F. oxysporum f. sp. fragariae.

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Biological Control of Fusarium Wilt of Strawberry by Nonpathogenic Fusarium oxysporum Isolated from Strawberry.

Cited by 24 publications

References 4 publications

Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenicFusarium oxysporumCS-20

Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenicFusarium oxysporumCS-20

Selective media for Fusarium oxysporum

Simple diagnosis using ethanol immersion of strawberry plants with latent infection by Colletotrichum acutatum, Dendrophoma obscurans, and Fusarium oxysporum f. sp. fragariae

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