Selective media for Fusarium oxysporum

Nishimura, Norio

doi:10.1007/s10327-007-0031-y

Cited by 28 publications

(19 citation statements)

References 11 publications

(11 reference statements)

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“…Inoculum bags were used to easily evaluate the effect of each treatment on the population density of the pathogen in soil. The number of initial viable propagules was measured by dilution on Fo-G1 agar, a medium selective for F. oxysporum (Nishimura 2007).…”

Section: Preparation Of Inoculummentioning

confidence: 99%

Fe2+ and Mn2+, potential agents to induce suppression of Fusarium oxysporum for biological soil disinfestation

Momma¹,

Kobara

Momma

2011

J Gen Plant Pathol

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Here Fusarium oxysporum was killed in exudates obtained from soil biologically disinfested with ethanol, indicating that physical interaction with soil microorganisms was not essential. Because acetic acid was confirmed to accumulated during the treatment, we evaluated the effect of acetic acid amendment against the pathogen in plastic containers. A drop in the soil redox potential seemed to be correlated with the fungicidal efficacy of acetic acid. Under reductive soil conditions, metal ions such as Mn 2? and Fe 2? formed, and the pathogen was effectively suppressed in Mn 2? and Fe 2? solution. Therefore, Fe 2? and Mn 2? may be the agents that induce suppression of the pathogen during biological soil disinfestation.

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Section: Preparation Of Inoculummentioning

confidence: 99%

Fe2+ and Mn2+, potential agents to induce suppression of Fusarium oxysporum for biological soil disinfestation

Momma¹,

Kobara

Momma

2011

J Gen Plant Pathol

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“…10 cm 9 7 cm). The initial viable propagules-the bud cells and chlamydospores-were counted using the dilution plate technique with F. oxysporum selective medium: Fo-G1 agar (Nishimura 2007). Fo-G1 contained KH 2 PO 4 , 1 g; KCl, 0.5 g; MgSO 4 Á7H 2 O, 0.5 g; ammonium citrate dibasic, 2 g; econazole nitrate, 5 mg (dissolved in 0.5 ml of dimethyl sulfoxide); H 3 BO 3 , 0.5 g; trace element solution, 0.2 ml (citric acid, 5 g; FeSO 4 Á7H 2 O, 5 g; ZnSO 4 Á7H 2 O, 1 g; CuSO 4 Á5H 2 O, 0.5 g; MnSO 4 Á5H 2 O, 0.5 g; Na 2 Mo 4 Á2H 2 O, 0.05 g to 95 ml of distilled water); chloramphenicol, 0.25 g; and agar, 20 g/1,000 ml of distilled water.…”

Section: Preparation Of Inoculummentioning

confidence: 99%

Biological soil disinfestation using ethanol: effect on Fusarium oxysporum f. sp. lycopersici and soil microorganisms

Momma¹,

Momma²,

Kobara

2010

J Gen Plant Pathol

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“…The number of wilt pathogens (Fusarium oxysporum) was determined by the dilution plate technique using a selective medium for Fusarium oxysporum (FD-G1 medium) (Takehara et al 2003;Nishimura 2007) for all soil samples collected every three days. Both aerobic and anaerobic bacteria were enumerated for soil samples collected at 18 d of all three pots as well as the original soil sample, by the dilution plate technique and the anaerobic roll tube method, respectively.…”

Section: Enumeration Of Populations Of Fusarium and Bacteria In The Smentioning

confidence: 99%

Development of anaerobic bacterial community consisted of diverse clostridial species during biological soil disinfestation amended with plant biomass

Mowlick

Hirota²,

Takehara

et al. 2012

Soil Science and Plant Nutrition

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Biological soil disinfestation (BSD) using plant biomass incorporation is an effective method and a good alternative to chemical fumigants for controlling soil-borne plant pathogens. In this study the bacterial communities in pot soil treated with three different BSD conditions (without plant biomass and with Brassica juncea L. plants or bran of wheat, Triticum aestivum L.) were analyzed using mainly molecular techniques. Earlier dropping of redox potential of both biomass-treated soils indicated rapid development of anaerobic conditions in the soil. The population of Fusarium oxysporum F. pathogen incorporated in the soil at the start was decreased considerably during the treatment, and the number of culturable anaerobic bacteria increased in both biomass-treated soils. Rather high concentrations of acetate and butyrate were detected from the biomass-treated soils. The polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis for the V3 region of 16S rRNA gene sequences revealed that the profiles of both biomass-treated soils were initially represented by similar and dominant groups, many of which were closely related to the species in the classes Clostridia and Bacilli of the phylum Firmicutes. Based on the clone library analysis, the control soil samples showed diverse bacterial groups with a few number of anaerobic clones. In contrast, for both biomass-treated libraries, clones belonging to the class Clostridia, a strictly anaerobic spore-forming bacterial group, appeared exceedingly dominant. The clostridial group detected was composed of phylogenetically diverse members, and it seemed likely that the diverse clostridial species were responsible for suppression of pathogens by making various compounds including volatile fatty acids and other compounds during anaerobic decomposition of plant biomass.

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Selective media for Fusarium oxysporum

Cited by 28 publications

References 11 publications

Fe2+ and Mn2+, potential agents to induce suppression of Fusarium oxysporum for biological soil disinfestation

Fe2+ and Mn2+, potential agents to induce suppression of Fusarium oxysporum for biological soil disinfestation

Biological soil disinfestation using ethanol: effect on Fusarium oxysporum f. sp. lycopersici and soil microorganisms

Development of anaerobic bacterial community consisted of diverse clostridial species during biological soil disinfestation amended with plant biomass

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