Biological control of bacterial spot of tomato by saprobe fungi from semi-arid areas of northeastern Brazil

Peitl, Douglas Casaroto; Araújo, Felipe André; Gonçalves, Ricardo Marcelo; Sumida, Ciro Hideki; Balbi-Peña, María Isabel

doi:10.5433/1679-0359.2017v38n3p1251

Cited by 7 publications

(3 citation statements)

References 15 publications

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“…were isolated from the roots of Sanqi ginseng and exerted antimicrobial activity against Alternaria (35), which may act as a potential biological control agent against Sanqi ginseng root rot disease. The genus Dictyochaeta, known as a prevalent endophytic fungus, is able to produce a mixture of volatile organic compounds that are lethal to plant-pathogenic fungi and bacteria (37).…”

Section: Discussionmentioning

confidence: 99%

Autotoxic Ginsenoside Disrupts Soil Fungal Microbiomes by Stimulating Potentially Pathogenic Microbes

Dai

Wang

et al. 2020

Appl Environ Microbiol

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Autotoxic ginsenosides have been implicated as one of the major causes for replant failure of Sanqi ginseng (Panax notoginseng); however, the impact of autotoxic ginsenosides on the fungal microbiome, especially on soilborne fungal pathogens, remains poorly understood. In this study, we aimed to investigate the influence of the ginsenoside monomers Rg1, Rb1, and Rh1, and that of their mixture (Mix), on the composition and diversity of the soil fungal community, as well as on the abundance and growth of the soilborne pathogen Fusarium oxysporum in pure culture. The addition of autotoxic ginsenosides altered the composition of the total fungal microbiome, as well as the taxa within the shared and unique treatment-based components, but did not alter alpha diversity (α-diversity). In particular, autotoxic ginsenosides enriched potentially pathogenic taxa, such as Alternaria, Cylindrocarpon, Gibberella, Phoma, and Fusarium, and decreased the abundances of beneficial taxa such as Acremonium, Mucor, and Ochroconis. Relative abundances of pathogenic taxa were significantly and negatively correlated with those of beneficial taxa. Among the pathogenic fungi, the genus Fusarium was most responsive to ginsenoside addition, with the abundance of Fusarium oxysporum consistently enhanced in the ginsenoside-treated soils. Validation tests confirmed that autotoxic ginsenosides promoted mycelial growth and conidial germination of the root rot pathogen F. oxysporum. In addition, the autotoxic ginsenoside mixture exhibited synergistic effects on pathogen proliferation. Collectively, these results highlight that autotoxic ginsenosides are capable of disrupting the equilibrium of fungal microbiomes through the stimulation of potential soilborne pathogens, which presents a significant hurdle in remediating replant failure of Sanqi ginseng. IMPORTANCE Sanqi ginseng [Panax notoginseng (Burk.) F. H. Chen] is geoauthentically produced in a restricted area of southwest China, and successful replanting requires a rotation cycle of more than 15 to 30 years. The increasing demand for Sanqi ginseng and diminishing arable land resources drive farmers to employ consecutive monoculture systems. Replant failure has severely threatened the sustainable production of Sanqi ginseng and causes great economic losses annually. Worse still, the acreage and severity of replant failure are increased yearly, which may destroy the Sanqi ginseng industry in the near future. The significance of this work is to decipher the mechanism of how autotoxic ginsenosides promote the accumulation of soilborne pathogens and disrupt the equilibrium of soil fungal microbiomes. This result may help us to develop effective approaches to successfully conquer the replant failure of Sanqi ginseng.

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Section: Discussionmentioning

confidence: 99%

Autotoxic Ginsenoside Disrupts Soil Fungal Microbiomes by Stimulating Potentially Pathogenic Microbes

Dai

Wang

et al. 2020

Appl Environ Microbiol

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“…actinidae which causes canker disease in kiwi fruit, and Pseudomonas syringae pv. tomato , which causes bacterial speck on tomatoes (Peitl et al 2017 ; Yu et al 2016 ).…”

Section: Indirect Mechanismsmentioning

confidence: 99%

Mechanisms of action of plant growth promoting bacteria

Olanrewaju

Glick

Babalola

2017

World J Microbiol Biotechnol

791

470

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The idea of eliminating the use of fertilizers which are sometimes environmentally unsafe is slowly becoming a reality because of the emergence of microorganisms that can serve the same purpose or even do better. Depletion of soil nutrients through leaching into the waterways and causing contamination are some of the negative effects of these chemical fertilizers that prompted the need for suitable alternatives. This brings us to the idea of using microbes that can be developed for use as biological fertilizers (biofertilizers). They are environmentally friendly as they are natural living organisms. They increase crop yield and production and, in addition, in developing countries, they are less expensive compared to chemical fertilizers. These biofertilizers are typically called plant growth-promoting bacteria (PGPB). In addition to PGPB, some fungi have also been demonstrated to promote plant growth. Apart from improving crop yields, some biofertilizers also control various plant pathogens. The objective of worldwide sustainable agriculture is much more likely to be achieved through the widespread use of biofertilizers rather than chemically synthesized fertilizers. However, to realize this objective it is essential that the many mechanisms employed by PGPB first be thoroughly understood thereby allowing workers to fully harness the potentials of these microbes. The present state of our knowledge regarding the fundamental mechanisms employed by PGPB is discussed herein.

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“…A. C. Almeida, Izabel, & Gusmão, 2011;Leão-Ferreira, Pascholati, Gusmão, & Castañeda Ruiz, 2013;Santa Izabel & Gusmão, 2018). Some of these saprobes were being used as potential biological control agents and resistance inducers (Resende, Milagres, Rezende, Aucique-Perez, & Rodrigues, 2015;Barros, Fonseca, Balbi-Peña, Pascholati, & Peitl, 2015;Rodríguez et al, 2016;Peitl et al, 2017;Ribeiro et al, 2018;Botrel et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Antagonism of saprobe fungi from semiarid areas of the Northeast of Brazil against Sclerotinia sclerotiorum and biocontrol of soybean white mold

Peitl

Sumida

Gonçalves

et al. 2020

Semina: Ciênc. Agrár.

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The antagonistic activity of 25 saprobe fungi from semiarid areas of Northeast Brazil was evaluated against Sclerotinia sclerotiorum (Lib.) de Bary (Helotiales: Sclerotiniaceae). Four fungi [Myrothecium sp. Tode (Hypocreales: Stachybotryaceae) isolate 2, Volutella minima Höhn. (Hypocreales: Nectriaceae), Phialomyces macrosporus P.C. Misra & P.H.B. Talbot (Pezizomycotina) and Dictyosporium tetraseriale Goh, Yanna & K.D. Hyde (Pleosporales: Dictyosporiaceae)] were selected and further tested their ability to inhibit mycelial growth, sclerotia formation and ascospore germination of S. sclerotiorum and to control white mold on soybean plants. V. minima and P. macrosporus filtrates at 50% effectively suppressed mycelial growth and Myrothecium sp. isolate 2 completely suppressed sclerotia formation and inhibited ascospore germination by over 95%, the same result as commercial fungicide fluazinam. Soybean plants pre-treated with Myrothecium sp. isolate 2, P. macrosporus, and V. minima and inoculated with S. sclerotiorum showed a reduction of 55.8%, 79.7%, and 83.2% of area under disease progress curve (AUDPC) of white mold, respectively, in relation to water. Collectively, these results underline the antagonistic activity of V. minima, P. macrosporus, and Myrothecium sp. isolate 2 against S. sclerotiorum and their potential as biocontrol agents of soybean white mold.

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Biological control of bacterial spot of tomato by saprobe fungi from semi-arid areas of northeastern Brazil

Cited by 7 publications

References 15 publications

Autotoxic Ginsenoside Disrupts Soil Fungal Microbiomes by Stimulating Potentially Pathogenic Microbes

Autotoxic Ginsenoside Disrupts Soil Fungal Microbiomes by Stimulating Potentially Pathogenic Microbes

Mechanisms of action of plant growth promoting bacteria

Antagonism of saprobe fungi from semiarid areas of the Northeast of Brazil against Sclerotinia sclerotiorum and biocontrol of soybean white mold

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