Biological clues to potent DNA-damaging activities in food and flavoring

Hossain, M. Zulfiquer; Gilbert, Sarah L.; Patel, Kalpesh; Ghosh, Soma; Bhunia, Anil K.; Kern, Scott E.

doi:10.1016/j.fct.2013.01.058

Cited by 18 publications

(20 citation statements)

References 73 publications

(116 reference statements)

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“…3a). In order to detect whether CdCl 2 affected placental DNA repair system of pregnant rats, we chose two typical molecular markers of DNA damage/repair pathway-APE1 [17] and p53 [18,19]-as our observational indexes and determined their expressions at both the mRNA and protein levels. RTqPCR and western blot suggested much stronger signals for APE1 and p53 in placenta tissues of 0.25 Cd group (Fig.…”

Section: Cdcl 2 Increased the Generation Of 8-ohdg In Placenta Tissuementioning

confidence: 99%

Increased Oxidative DNA Damage in Placenta Contributes to Cadmium-Induced Preeclamptic Conditions in Rat

Zhang

Lin

et al. 2015

Biol Trace Elem Res

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To explore the possible mechanisms of cadmium (Cd)-induced preeclamptic conditions in rats. In the present study, we introduced the in vivo model of preeclampsia by giving intraperitoneal injections of cadmium chloride (CdCl2) to pregnant rats from gestational day (GD) 4 to 19. Maternal body weights were recorded on GD 0, 14, and 20, while their systolic blood pressures (SBPs) monitored on GD 3, 11, and 18. On GD 20, rats were sacrificed and the specimens were collected. The morphological changes of placenta and kidney tissues of pregnant rats were examined by hematoxylin and eosin staining assay. Blood Cd level was detected by inductively coupled plasma mass spectrometry. Total antioxidant capacity (TAC) was evaluated using FRAP method and total nitrite (NOx) was detected with Griess reagent. Antioxidative factors and DNA damage/repair biomarkers were measured by real-time qPCR, western blot or immunohistochemistry study. The current results showed that CdCl2-treated pregnant rats developed preeclampsia (PE)-like manifestations, such as hypertension, albuminuria, with decreased TAC and increased blood Cd level, and pro-oxidative/antioxidative or DNA damage/repair biomarkers. Our study demonstrated that increased oxidative DNA damage in placenta could contribute to Cd-induced preeclamptic conditions in rat.

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Section: Cdcl 2 Increased the Generation Of 8-ohdg In Placenta Tissuementioning

confidence: 99%

Increased Oxidative DNA Damage in Placenta Contributes to Cadmium-Induced Preeclamptic Conditions in Rat

Zhang

Lin

et al. 2015

Biol Trace Elem Res

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“…We recently evaluated potent strand-breaking genotoxic activities in coffee, tea, and liquid smoke flavoring (Hossain et al, 2013). Liquid smoke induced DNA double-strand breaks (Hossain et al, 2013) as detected in the p53R assay and neutral comet assay as well as DNA single-strand breaks in rat gastric mucosa in vivo (Ohshima et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…Liquid smoke induced DNA double-strand breaks (Hossain et al, 2013) as detected in the p53R assay and neutral comet assay as well as DNA single-strand breaks in rat gastric mucosa in vivo (Ohshima et al, 1989). Flavonoids are well described to inhibit DNA topoisomerases (Bandele et al, 2008; Neukam et al, 2008; Lopez-Lazaro et al, 2011; Shiomi et al, 2013; Yoshida et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Flavonoids are well described to inhibit DNA topoisomerases (Bandele et al, 2008; Neukam et al, 2008; Lopez-Lazaro et al, 2011; Shiomi et al, 2013; Yoshida et al, 2013). Constituent pyrogallol-like polyphenols (PLPs) such as EGCG (found in green tea), gallic acid (green tea, black tea, and coffee), and pyrogallol (green tea, black tea, coffee, and liquid smoke) caused DNA strand breaks (Hossain et al, 2013). Because potential consequences of such potent DNA-damaging activity include cellular toxicity, mutagenesis, and carcinogenesis, it is relevant that damage occurred at concentrations consumed dietarily: etoposide near 5 μg/ml produced responses similar to a 1:1000 dilution of liquid smoke or a 1:20 dilution of coffee.…”

Section: Introductionmentioning

confidence: 99%

“…We hypothesized that physiologically relevant proteins might have a protective role against DNA-damaging agents from the diet. We tested candidate proteins for their ability to inhibit DNA-damage response in the p53R assay, a well characterized cellular biological assay sensitive to DNA strand breaks (Sohn et al, 2002; Cunningham et al, 2004; Gallmeier et al, 2005; Hossain et al, 2013;). This assay utilizes a human cell line in which luciferase expression is driven by a stably integrated p53 reporter construct.…”

Section: Introductionmentioning

confidence: 99%

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Salivary α-amylase, serum albumin, and myoglobin protect against DNA-damaging activities of ingested dietary agents in vitro

Hossain¹,

Patel²,

Kern³

2014

Food and Chemical Toxicology

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Potent DNA-damaging activities were seen in vitro from dietary chemicals found in coffee, tea, and liquid smoke. A survey of tea varieties confirmed genotoxic activity to be widespread. Constituent pyrogallol-like polyphenols (PLPs) such as epigallocatechin-3-gallate (EGCG), pyrogallol, and gallic acid were proposed as a major source of DNA-damaging activities, inducing DNA double-strand breaks in the p53R assay, a well characterized assay sensitive to DNA strand breaks, and comet assay. Paradoxically, their consumption does not lead to the kind of widespread cellular toxicity and acute disease that might be expected from genotoxic exposure. Existing physiological mechanisms could limit DNA damage from dietary injurants. Serum albumin and salivary α-amylase are known to bind EGCG. Salivary α-amylase, serum albumin, and myoglobin, but not salivary proline-rich proteins, reduced damage from tea, coffee, and PLPs, but did not inhibit damage from the chemotherapeutics etoposide and camptothecin. This represents a novel function for saliva in addition to its known functions including protection against tannins. Cell populations administered repeated pyrogallol exposures had abatement of measured DNA damage by two weeks, indicating an innate cellular adaptation. We suggest that layers of physiological protections may exist toward natural dietary products to which animals have had high-level exposure over evolution.

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Associations of coffee and tea consumption with lung cancer risk

Zhu

Smith‐Warner

et al. 2020

Intl Journal of Cancer

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Associations of coffee and tea consumption with lung cancer risk have been inconsistent, and most lung cancer cases investigated were smokers. Included in this study were over 1.1 million participants from 17 prospective cohorts. Cox regression analyses were conducted to estimate hazard ratios (HRs) and 95% confidence intervals (CIs). Potential effect modifications by sex, smoking, race, cancer subtype and coffee type were assessed. After a median 8.6 years of follow‐up, 20 280 incident lung cancer cases were identified. Compared with noncoffee and nontea consumption, HRs (95% CIs) associated with exclusive coffee drinkers (≥2 cups/d) among current, former and never smokers were 1.30 (1.15‐1.47), 1.49 (1.27‐1.74) and 1.35 (1.15‐1.58), respectively. Corresponding HRs for exclusive tea drinkers (≥2 cups/d) were 1.16 (1.02‐1.32), 1.10 (0.92‐1.32) and 1.37 (1.17‐1.61). In general, the coffee and tea associations did not differ significantly by sex, race or histologic subtype. Our findings suggest that higher consumption of coffee or tea is associated with increased lung cancer risk. However, these findings should not be assumed to be causal because of the likelihood of residual confounding by smoking, including passive smoking, and change of coffee and tea consumption after study enrolment.

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Biological clues to potent DNA-damaging activities in food and flavoring

Cited by 18 publications

References 73 publications

Increased Oxidative DNA Damage in Placenta Contributes to Cadmium-Induced Preeclamptic Conditions in Rat

Increased Oxidative DNA Damage in Placenta Contributes to Cadmium-Induced Preeclamptic Conditions in Rat

Salivary α-amylase, serum albumin, and myoglobin protect against DNA-damaging activities of ingested dietary agents in vitro

Associations of coffee and tea consumption with lung cancer risk

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