Biological, Chemical, and Physical Compatibility of Chitosan and Biopharmaceuticals

Fujita, Masanori; Kishimoto, Satoko; Hattori, Hidemi; Kanatani, Yasuhiro

doi:10.1002/9781119962977.ch6

Cited by 6 publications

(5 citation statements)

References 81 publications

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“…Chitin/chitosan is second most abundant natural nontoxic biomaterial, and is produced from the exoskeleton of sea food, shellfish, crabs, shrimps, insects, edible mushrooms, and sea weed algae [ 1 ]. The advantages in biochemical activities of chitin/chitosan-based materials include: anti-infectious activity [ 2 ]; stimulation of angiogenesis/wound repair; and stabilization/activation of growth factors [ 3 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites

et al. 2014

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BackgroundChitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).Materials and methodsCNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.ResultsThe TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.ConclusionsOur results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

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Section: Introductionmentioning

confidence: 99%

Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites

et al. 2014

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“…This positively charged chitosan can form complex ionic bonds by binding to negatively charged materials such as collagen. 29 This chitosan's cationic nature can stimulate cell adhesion as a modulator of cell morphology, differentiation, cell movement, cell synthesis, and function. 30 Another ingredient used in making scaffolds in this study is collagen.…”

Section: Discussionmentioning

confidence: 99%

The Chitosan-chicken Shank Collagen Used as Scaffold Through Lymphocyte Cell Proliferation in Bone Regeneration Process

2021

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Introduction. Alveolar bone defect in dentistry can be caused by injury after tooth extraction, periodontal disease, enucleation of a cyst, and tumor surgery. Scaffold in tissue engineering is an important material that can stimulate osteogenesis process. Lymphocyte cells have a role in promoting and accelerating the proliferation of supporting cells like osteoblast to accelerate the bone regeneration process.Objective. The purpose of this study was to determine the effect of chitosan-collagen chicken shank collagen used as scaffold for bone regeneration through lymphocyte cell proliferation.Method. Twelve Wistar rats (Rattus norvegicus) were prepared as animal models in this study. Bone defects are intentionally made in both the right and left femur bones of the rat. Total samples were 24 divided into four groups: Group 1 as a control Group using 3% CMC-Na, Group 2 using chitosan scaffold only, Group 3 using chitosanchicken shank collagen scaffold (50:50), and Group 4 using chitosan-chicken shank collagen scaffold (80:20). The animals were sacrificed on the 5 th day, and histopathological examination was carried out to observe the number of lymphocyte cells.Results. Significant differences between all groups can be showed in the one-way ANOVA test (p value>0.05). The highest lymphocyte cells were found in Group 3 with chitosan-chicken shank collagen scaffold (50:50). Conclusion.The chitosan-chicken shank collagen used as scaffold can increase the bone regeneration process through increased lymphocyte cell proliferation.

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“…Photocrosslinkable chitosan (Az-CH-LA) in DMEM/F12 medium is a viscous solution that crosslinks upon UV irradiation, resulting in insoluble photocrosslinked chitosan hydrogels (PCH). Substantial effects of PCH have been observed during the formation of granulation tissue and vascularization in vivo and during neutrophil infiltration and biodegradation of PCH [12,24]. In a previous study, we demonstrated the use of PCH as a submucosal injection agent in rats [26].…”

Section: Hydrogels For Mucosal Elevation In Endoscopic Mucosal Resectmentioning

confidence: 95%

“…However, production of chitosan from the shells of crustaceans or shrimp from food industry waste is economically feasible. Chitosan comprises N-acetylglucosamine and glucosamine, can be obtained by alkaline deacetylation of chitin, and is non-toxic and biocompatible with living tissue [11,12]. The production of chitin from shells predominantly involves the removal of various proteins and dissolution of the calcium carbonate.…”

Section: Introductionmentioning

confidence: 99%

Application of hydrogels as submucosal fluid cushions for endoscopic mucosal resection and submucosal dissection

2015

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Numerous new techniques have recently been reported and described for the endoscopic mucosal resection (EMR) of large superficial lesions of the gastrointestinal tract, using various natural, synthetic, and semi-synthetic materials such as chitin/chitosan and their derivatives. Although saline-assisted EMR is an established minimally invasive therapy, en bloc resection and histopathological analyses are required to determine its curative potential. In addition, complete resection of lesions of >2 cm in diameter remains difficult, despite improved EMR techniques. The development of endoscopic submucosal dissection (ESD) has increased dissection rates for en bloc resection of large lesions, but perforation occurs more frequently during ESD than during EMR. Submucosal injections of those biomaterials which have high viscosity and hydrogelatinization ability as submucosal fluid cushions (SFC) may facilitate ESD as well as EMR for the treatment of superficial tumors of the alimentary tract. In this review, we describe the application of biomaterials such as chitosan derivatives, sodium hyaluronate, and 50% glucose as a SFC for ESD, focusing photocrosslinked chitosan hydrogels (PCH) which we have originally developed.

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Biological, Chemical, and Physical Compatibility of Chitosan and Biopharmaceuticals

Cited by 6 publications

References 81 publications

Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites

Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites

The Chitosan-chicken Shank Collagen Used as Scaffold Through Lymphocyte Cell Proliferation in Bone Regeneration Process

Application of hydrogels as submucosal fluid cushions for endoscopic mucosal resection and submucosal dissection

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