Biological characterization of <i>Tomato leaf curl New Delhi virus</i> from Spain

Ruiz, Leticia; Simón, Almudena; Velasco, L.; Janssen, Dirk

doi:10.1111/ppa.12587

Cited by 29 publications

(39 citation statements)

References 32 publications

(48 reference statements)

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“…Furthermore, these same authors recently obtained an infectious clone for one of the isolates collected from zucchini squash in Almería (isolate ToLCNDV-[ES-Alm-Zucchini-13]) and demonstrated that it was able to infect readily and induce symptoms in tomato [43]. Sequence comparison indicated that few nucleotide differences scattered in the genome are observed between the isolate cloned by Ruiz et al [43] and the one for which an infectious clone was obtained here. Based on the infectious clones available, further research then might help to localize viral genetic determinants associated with infection and symptom induction in tomato.…”

Section: Discussionmentioning

confidence: 64%

“…This might be the case of ToLCNDV isolates detected in Spain, which are closely related genetically and exhibit a recombinant origin of their DNA-A. This group of isolates differed at the strain level, and the name “Spain (ES)” was proposed for the strain [16,43]. Isolates closely associated with this strain also have been reported to cause severe epidemics in cucurbits in Tunisia and Italy [17,19,20,21], suggesting the emergence of the ES strain in the western Mediterranean Basin.…”

Section: Discussionmentioning

confidence: 99%

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A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin

Fortes

Sánchez-Campos

Fiallo‐Olivé

et al. 2016

Viruses

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Tomato leaf curl New Delhi virus (ToLCNDV) is a whitefly-transmitted bipartite begomovirus (genus Begomovirus, family Geminiviridae) that causes damage to multiple cultivated plant species mainly belonging to the Solanaceae and Cucurbitaceae families. ToLCNDV was limited to Asian countries until 2012, when it was first reported in Spain, causing severe epidemics in cucurbit crops. Here, we show that a genetically-uniform ToLCNDV population is present in Spain, compatible with a recent introduction. Analyses of ToLCNDV isolates reported from other parts of the world indicated that this virus has a highly heterogeneous population genetically with no evident geographical, plant host or year-based phylogenetic groups observed. Isolates emerging in Spain belong to a strain that seems to have evolved by recombination. Isolates of this strain seem adapted to infecting cucurbits, but poorly infect tomatoes.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin

Fortes

Sánchez-Campos

Fiallo‐Olivé

et al. 2016

Viruses

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“…In fact, ToLCNDV-ES has been reported as an important threat for cucurbit crops in Mediterranean countries, where it has recently emerged. In spite of that, field incidence and symptoms of ToLCNDV-ES are lower in tomato than in zucchini crops (Fortes et al 2016;Ruiz et al 2017).…”

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confidence: 95%

“…Recently, the virus invaded the Mediterranean countries of Spain (Juárez et al 2014), Tunisia (Mnari-Hattab et al 2015), and Italy (Panno et al 2016). The latest studies of ToLCNDV from Spanish isolates provided evidence that it is a new strain, designated ToLCNDV-ES, that may have evolved by recombination (Fortes et al 2016;Ruiz et al 2017). Symptoms produced by this strain in zucchini are curling, chlorosis, and vein thickening of leaves; stunted growth; and fruit deformation and abortion; whereas, in tomato, the symptoms of chlorosis and deformation in leaves are reminiscent of tomato yellow leaf curl disease (Ruiz et al 2017).…”

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confidence: 99%

Absolute Quantification ofTomato leaf curl New Delhi virusSpain strain, ToLCNDV-ES: Virus Accumulation in a Host-Specific Manner

et al. 2018

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Tomato leaf curl New Delhi virus (ToLCNDV) (family Geminiviridae, genus Begomovirus) has recently been introduced in western Mediterranean countries. Isolates in Spain constitute a new strain, denominated ToLCNDV-ES, that is causing losses in commercial zucchini and melon crops; however, it is also, although less often, detected in commercial tomato crops. We developed a tissue-print hybridization test to detect the two genomic components of the virus and a TaqMan quantitative polymerase chain reaction (qPCR) test to estimate the number of genome copies in plants. qPCR was approximately 104 to 106 times more sensitive than tissue-print hybridization to detect viral genomic DNA-A and DNA-B, respectively. It also detected the virus in more experimentally and naturally ToLCNDV-ES-infected zucchini squash and tomato plants. ToLCNDV-ES DNA-A titers were significantly lower in tomato than in zucchini plants, often falling below the detection limits in the hybridization test. In addition, the DNA-B accumulation was impaired in tomato when compared with zucchini. According to the data obtained in this study, the differences in viral titers of both plant species contribute to explain the dissimilarities in symptom expression, capability of detection, and transmission of the virus.

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“…For SALCV, SB26/SB29 and SB41, very limited information is available and further studies are needed to clarify their identity. Begomoviruses are phloem-limited viruses and therefore generally considered not seed-transmitted, with a few possible exceptions (Mink, 1993;Bos, 1999) Yes Reported to be persistently transmitted by Bemisia tabaci Rosen et al, 2015) ToLCNDV Cannot be excluded Not reported in potato, but one study reported a ToLCNDV strain to be seed transmitted in chayote (Sechium edule) (Sangeetha et al, 2018) Yes Reported to be persistently transmitted by Bemisia tabaci (Rosen et al, 2015;Ruiz et al, 2017) ToMHaV No Not reported. The identity is not established, but the virus has been shown to be transmissible and to produce consistent symptoms (Hooker et al, , 1985 3.1.2.…”

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confidence: 99%

Pest categorisation of non‐EU viruses and viroids of potato

Bragard¹,

Dehnen‐Schmutz²,

Gonthier³

et al. 2020

EFS2

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Following a request from the EU Commission, the Panel on Plant Health has addressed the pest categorisation of those viruses and viroids (hereafter referred to as viruses) of Solanum tuberosum and other tuber-forming Solanum spp. (hereafter referred to as potato) which are considered to be either non-EU or of undetermined standing based on a previous EFSA opinion. These viruses belong to different families and genera and either have an established identity or produce consistent symptoms. Plants for planting is the main pathway for entry for all categorised viruses as they can all be transmitted by vegetative propagation. Several categorised viruses have a relatively wide host range and/or are vector-transmitted, increasing the potential for entry. The information currently available on geographical distribution, biology, epidemiology, impact and potential entry pathways has been evaluated with regard to the criteria to qualify as potential Union quarantine pest or as Union regulated non-quarantine pest (RNQP). Since this opinion addresses specifically the non-EU potato viruses, in general these viruses do not meet the criteria assessed by EFSA to qualify as potential Union regulated non-quarantine pests. The following viruses meet the criteria to qualify as potential Union quarantine pest: APLV, APMMV, APMoV, ChiLCV, CYSDV, PAMV, PBRSV, PVH, PVP, PVT, PYDV, PYMV, PYV, PYVV, RCVMV, SALCV, SB26/29, ToCV, ToLCNDV, ToMHaV, ToMoTV, ToSRV and ToYVSV. With the exception of the criterion regarding the potential for consequences in the EU territory, for which the Panel is unable to conclude because of lack of information, AVB, CPSbV, PaLCrV, PapMV, PVB, PVU, SB41 and TVBMV meet all the other criteria to qualify as potential Union quarantine pest. PotLV and WPMV do not qualify as potential Union quarantine pest, since they are not reported to have any impact. For most of the categorised viruses, the conclusions of the Panel have inherent uncertainties, due to the lack of quantitative data on their impact and/or absence or limited availability of information on the biology, epidemiology and geographical distribution.

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Biological characterization of Tomato leaf curl New Delhi virus from Spain

Cited by 29 publications

References 32 publications

A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin

A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin

Absolute Quantification ofTomato leaf curl New Delhi virusSpain strain, ToLCNDV-ES: Virus Accumulation in a Host-Specific Manner

Pest categorisation of non‐EU viruses and viroids of potato

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