Biological characterization of Fusobacterium necrophorum. Cell fractions in preparation for toxin and immunization studies

García, Manuel; Alexander, Danny; McKay, K. A.

doi:10.1128/iai.11.4.609-616.1975

Cited by 37 publications

(6 citation statements)

References 14 publications

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“…Preparation of cell walls and water-soluble and phenol-soluble lipopolysaccharide preparations. Cell walls were prepared according to the procedure of Garcia et al (8), using a sonicated preparation of F. nucleatum as described earlier. Water-soluble and phenol-soluble lipopolysaccharide preparations followed the procedure in Weir (25), using acetonedried whole cells.…”

Section: Methodsmentioning

confidence: 99%

Hemagglutinating activity of Fusobacterium nucleatum

Falkler¹,

Hawley²

1977

Infect Immun

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Gingival isolates of oral Fusobacterium nucleatum strains (gram-negative anaerobic fusiform bacilli) have shown the characteristic ability to hemagglutinate a variety of erythrocytes (RBC) of human and animal origin. Other members of the genus tested (F. necrophorus, F. varium, and F. mortiferum) displayed little if any ability to hemagglutinate RBC. The hemagglutination (HA) activity could be observed in the F. nucleatum strains with the whole cells and in most instances with sonicated preparations of the organisms. The HA activity was observed in cell wall preparations of the organism and appeared dependent upon a heat-labile protein component of the cell wall. In decreasing order, the RBC that would hemagglutinate with the smallest concentration of HA preparations were rabbit, monkey, human, sheep, horse, and ox. No differences in HA activity of the preparations with cells from the various human blood types were noted. Absorption of the HA preparation of one strain with human cells removed activity when tested with sheep cells, suggesting similar types of receptors. The HA moiety was bound to the cells via a Ca2+ binding site interaction since ethylenediaminetetraacetic acid and ethylene glycol-bis-N,N'-tetraacetic acid inhibited binding, and HA could be reestablished by the addition of Ca2+ but not

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Section: Methodsmentioning

confidence: 99%

Hemagglutinating activity of Fusobacterium nucleatum

Falkler¹,

Hawley²

1977

Infect Immun

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“…Several investigators have attempted to induce protective immunity against F. necrophorum by using a variety of antigenic components. Attempts to induce protective immunity have included the use of whole-cell cultures (Roberts, 1970;Abe et al, 1976a;Cameron and Fuls, 1977;Conlon et al, 1977;Smith et al, 1985Smith et al, , 1989, cytoplasmic fractions (Garcia et al, 1974(Garcia et al, , 1975aGarcia and McKay, 1978), lipopolysaccharides (Garcia et al, 1974(Garcia et al, , 1975aAbe et al, 1976a;Cameron and Fuls, 1977;Conlon et al, 1977;Smith et al, 1985Smith et al, , 1989, outer membrane proteins (Emery and Vaughn, 1986), leukotoxins (Roberts, 1970;Garcia et al, 1975a;Clarke et al, 1986;Emery et al, 1986a,b;Saginala et al, 1996a,b), and culture supernatants (Jensen et al, 1954c;Takeuchi et al, 1984;Saginala et al, 1996aSaginala et al, ,b, 1997. Efficacy has varied from nil to significant protection.…”

Section: Itemmentioning

confidence: 99%

Liver abscesses in feedlot cattle: a review.

Nagaraja

Chengappa

1998

Journal of Animal Science

331

194

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Liver abscesses in slaughtered beef cattle result from aggressive grain-feeding programs. The incidence, averaging from 12 to 32% in most feedlots, is influenced by a number of dietary and management factors. Liver abscesses represent a major economic liability to producers, packers, and ultimately consumers. Besides liver condemnation, economic impacts include reduced feed intake, reduced weight gain, decreased feed efficiency, and decreased carcass yield. Fusobacterium necrophorum, a member of the ruminal anaerobic bacterial flora, is the primary etiologic agent. Actinomyces pyogenes is the second most frequently isolated pathogen. Ruminal lesions resulting from acidosis generally are accepted as the predisposing factors for liver abscesses. F. necrophorum possesses or produces a number of virulence factors that participate in the penetration and colonization of the ruminal epithelium and subsequent entry and establishment of infection in the liver. However, only a few virulence factors have been characterized well. Control of liver abscesses in feedlot cattle generally has depended on the use of antimicrobial compounds. Five antibiotics (i.e., bacitracin methylene disalicylate, chlortetracycline, oxytetracycline, tylosin, and virginiamycin) are approved for prevention of liver abscesses in feedlot cattle. Tylosin is the most effective and the most commonly used feed additive. Tylosin feeding reduces abscess incidence by 40 to 70%. The mode of action of antibiotics in preventing liver abscesses is believed to be via inhibition of ruminal F. necrophorum. Protective immunity against F. necrophorum induced by a variety of antigenic components has ranged from ineffectual to significant protection.

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“…F. necrophorum produces a leukotoxin (Beveridge, 1934;Roberts, 1967aRoberts, , 1967bGarcia et al, 1975a;Fales et al, 1977;Coyle-Dennis and Lauerman, 1978;Tan et al, 1994) which is active against a variety of white blood cells, especially polymorphonuclear neutrophiles, which could impede the local immune response at the site of infection. P. intermedia could add to tissue destruction with its ability to degrade lipid materials ) and the production of proteolytic enzymes such as dipeptidyl peptidases and cysteine proteases, the latter also involved in the breakdown of IgG (Slots, 1981;Jansen et al, 1995;Gazi et al, 1997).…”

Section: Pathogenicity Of F Necrophorum As a Trigger Organismmentioning

confidence: 99%

Microbiological understandings and mysteries of noma (cancrum oris)

1999

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The microbiologic history of noma was reviewed. Studies have associated the disease process with large numbers of fusiform bacilli and spirochetal organismS. In order to study the microbiology of the staging and infection periods of noma 62 Nigerian children, aged 3–14 years, 22 children had acute necrotizing ulcerative gingivitis (ANUG) and were also malnourished, 20 exhibited no acute necrotizing ulcerative gingivitis but were malnourished and 20 were free of acute necrotizing ulcerative gingivitis and in good nutritional state) were evaluated for the presence of viruses and oral microorganisms. The ANUG cases in the malnourished children had a higher incidence of Herpesviridae, the main virus being detected was cytomegalovirus. There were more anaerobic microorganisms recovered, with Prevotella intermedia as the predominant isolate, in the malnourished children as compared to the healthy children. A study of the predominant microflora in active sites of noma lesions was carried out in eight noma patients, 3–15 years of age, in Sokoto State, northwestern Nigeria.Fusobacterium necrophorum was recovered from 87.5% of the noma lesionS. Oral microorganisms isolated included Prevotella intermedia, alpha‐hemolytic streptococci and Actinomyces spp.which were isolated from 75.0, 50.0 and 37.5% of the patients, respectively.Peptostreptococcus micros, Veil‐lonella parvula, Staphylococcus aureus and Pseudomonas spp.were each recovered from one lesion. All strains were observed to be sensitive to all of the antibiotics tested with the exception of one strain of P. Intermedia which showed resistance to penicillin. The pathogenic mechanisms of F.necrophorum as a trigger organism were discussed. The isolation from human noma lesions of F.necrophorum, a pathogen primarily associated with animal diseases, may have important etiologic and animal transmission implications.

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Biological characterization of Fusobacterium necrophorum. Cell fractions in preparation for toxin and immunization studies

Cited by 37 publications

References 14 publications

Hemagglutinating activity of Fusobacterium nucleatum

Hemagglutinating activity of Fusobacterium nucleatum

Liver abscesses in feedlot cattle: a review.

Microbiological understandings and mysteries of noma (cancrum oris)

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