Biological characteristics and genomic analysis of a Stenotrophomonas maltophilia phage vB_SmaS_BUCT548

Zhang

et al. 2022

IJMS

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The spread of multidrug-resistant Klebsiella pneumoniae (MDR-KP) has become an emerging threat as a result of the overuse of antibiotics. Bacteriophage (phage) therapy is considered to be a promising alternative treatment for MDR-KP infection compared with antibiotic therapy. In this research, a lytic phage BUCT610 was isolated from hospital sewage. The assembled genome of BUCT610 was 46,774 bp in length, with a GC content of 48%. A total of 83 open reading frames (ORFs) and no virulence or antimicrobial resistance genes were annotated in the BUCT610 genome. Comparative genomics and phylogenetic analyses showed that BUCT610 was most closely linked with the Vibrio phage pYD38-A and shared 69% homology. In addition, bacteriophage BUCT610 exhibited excellent thermal stability (4–75 °C) and broad pH tolerance (pH 3–12) in the stability test. In vivo investigation results showed that BUCT610 significantly increased the survival rate of Klebsiella pneumonia-infected Galleria mellonella larvae from 13.33% to 83.33% within 72 h. In conclusion, these findings indicate that phage BUCT610 holds great promise as an alternative agent with excellent stability for the treatment of MDR-KP infection.

Section: Methodsmentioning

confidence: 99%

Characterization and Comparative Genomics Analysis of a New Bacteriophage BUCT610 against Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae

Zhang

et al. 2022

IJMS

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“…BUCT603 formed large, clear and irregular circular plaques (~3 mm in diameter) after incubation on the lawn of SMA118 for 16 h at 37°C (Figure 3A). Several other studies have also reported the presence of S. maltophilia lytic phages in sewage (Fan et al, 2012;Huang et al, 2012;Zhang et al, 2021) indicating that hospital sewage provides a resource for the isolation of phages directed against drug-resistant bacterium.…”

Section: Isolation and Morphology Of Bacteriophage Buct603mentioning

confidence: 91%

Characterization of the Bacteriophage BUCT603 and Therapeutic Potential Evaluation Against Drug-Resistant Stenotrophomonas maltophilia in a Mouse Model

Zhang

et al. 2022

Front. Microbiol.

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Stenotrophomonas maltophilia (S. maltophilia) is a common opportunistic pathogen that is resistant to many antibiotics. Bacteriophages are considered to be an effective alternative to antibiotics for the treatment of drug-resistant bacterial infections. In this study, we isolated and characterized a phage, BUCT603, infecting drug-resistant S. maltophilia. Genome sequencing showed BUCT603 genome was composed of 44,912 bp (32.5% G + C content) with 64 predicted open reading frames (ORFs), whereas no virulence-related genes, antibiotic-resistant genes or tRNA were identified. Whole-genome alignments showed BUCT603 shared 1% homology with other phages in the National Center for Biotechnology Information (NCBI) database, and a phylogenetic analysis indicated BUCT603 can be classified as a new member of the Siphoviridae family. Bacteriophage BUCT603 infected 10 of 15 S. maltophilia and used the TonB protein as an adsorption receptor. BUCT603 also inhibited the growth of the host bacterium within 1 h in vitro and effectively increased the survival rate of infected mice in a mouse model. These findings suggest that bacteriophage BUCT603 has potential for development as a candidate treatment of S. maltophilia infection.

“…3015 was isolated from sewage samples collected from the Clinical Laboratory center, Taian City Central Hospital. Microbial diversity sequencing is based on a second-generation high-throughput technology to sequence the 16S rRNA genes [7]. Primers were used to amplify the 16S rRNA genes, and the puri ed PCR product could be sequenced with an Illumina MiSeq sequencer (San Diego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Biological Characteristics And Genomic Analysis of A Novel Bacteriophage BUCT609 Infecting Stenotrophomonas Maltophilia

Fan

et al. 2021

Preprint

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Stenotrophomonas maltophilia is widely distributed in nature and has a high isolation rate in nosocomial infections. Due to its potential application and important role in clinical practice, the relevant studies of S.maltophilia have received much attention. S.maltophilia phage BUCT609 (GenBank: MW960043) was isolated from hospital sewage with S.maltophilia strain No. 3015 as a host. Morphologically, it can be inferred as Podoviridae phage from the result of transmission electron microscopy (TEM). The electron microscopy also shows that the phage has an isometric capsid ~50 nm in diameter. The one-step growth curve demonstrated that the incubation period of 10 min and the burst size is 382 pfu/cell when its optimal multiplicity of infection (MOI) is 0.01. Phage BUCT609 had a high survival rate at pH 3 to 10 and tolerant temperature from 4 ℃ to 55 ℃. Next-Generation Sequencing (NGS) results demonstrated that its complete genome is linear double-stranded DNA of 43145 bp in length, and the GC content is 58 %. It has very little resemblance to other phages. The BlastN analysis shows that the genome of phage BUCT609 shares 22 % homology with S.maltophilia phage Ponderosa (GenBank: MK903280.1), and it encodes 56 putative proteins, of which only 25 have annotated function. Phage BUCT609 with a relatively large burst size and excellent survival ability in a wide pH and temperature range, suggests BUCT609 is a potential alternative for multi-drug resistance S.maltophilia therapy.