Glioma-initiating cells (GIC) are glioma stem-like cells that contribute to glioblastoma (GBM) development, recurrence, and resistance to chemotherapy and radiotherapy. They have recently become the focus of novel treatment strategies. Cyclophilin A (CypA) is a cytosolic protein that belongs to the peptidyl-prolyl isomerase (PPIase) family and the major intracellular target of the immunosuppressive drug cyclosporin A (CsA). In this study, we investigate the functions of CypA and its mechanism of action in GICs' development. We analyzed differences in CypA expression between primary tumors and neurospheres from the GDS database, both before and after GIC differentiation. A series of experiments was conducted to investigate the role of CypA in GIC stemness, self-renewal, proliferation, radiotherapy resistance, and mechanism. We then designed glutathione S-transferase (GST) pulldown and coimmunoprecipitation assays to detect signaling activity. In this study, we demonstrated that CypA promotes GIC stemness, self-renewal, proliferation, and radiotherapy resistance. Mechanistically, we found that CypA binds β-catenin and is recruited to Wnt target gene promoters. By increasing the interaction between β-catenin and TCF4, CypA enhances transcriptional activity. Our results demonstrate that CypA enhances GIC stemness, self-renewal, and radioresistance through Wnt/β-catenin signaling. Due to its promotive effects on GICs, CypA is a potential target for future glioma therapy. .
Stenotrophomonas maltophilia (S. maltophilia) is widely distributed in nature and frequently causes nosocomial infections. In this work, the biological characteristics and genome of a new S. maltophilia phage BUCT609 isolated from hospital sewage with S. maltophilia strain No. 3015 as host was analyzed and its therapeutic effect in vivo was explored. It was observed by TEM that phage BUCT609 belongs to the Podoviridae with a 10 nm tail structure and a capsid with a diameter of about 50 nm. It has a short latent period (about 10 min) and its burst size is 382 PFU /cell when multiplicity of infection (MOI) is 0.01. Furthermore, it has a high survival rate in the environment with a pH range from 3 to 10 and temperature range from 4°C to 55°C. The complete genome of phage BUCT609 is linear double-stranded DNA of 43,145 bp in length, and the GC content is 58%. The genome sequence of phage BUCT609 shares <45% homology with other phages. No virulence genes and antibiotic resistance genes were found in bacteriophage BUCT609. In vivo animal experiments showed that the survival rate of mice infected with S. maltophilia was significantly improved after the intranasal injection of phage BUCT609. Therefore, our study supports that phage BUCT609 could be used as a promising antimicrobial candidate for treating S. maltophilia infections.
A system known as pedestrian recognition makes use of several cameras to identify the surrounding area and quickly identify and match the target demographic. Based on pedestrian recognition, the picture model, pedestrian features, and other information, the features are developed to have a high degree of generalizability, distinctiveness, and accuracy. e application approach for pedestrian re-recognition based on deep learning for numerous features is proposed in this paper. e suggested approach successfully preserves high-level semantic information, which helps network members extract all of the pedestrian properties. As external material and semantic information were combined horizontally and vertically, environmental interference was decreased, and people's ability to create networks was enhanced. e voice channel of the speech system was introduced in order to fully utilize the global information network, and the connection between the channels was carefully addressed in order to enhance the global information network's capacity for expression. e null convolution reduced the operational continuity of the identication information. To increase the consistency of the data, the multi-level spatial convolution structure was merged with the entire image in this paper. After numerous experiments, the three groups were 89.5%, 89.5%, and 89.1%, respectively, compared to 1501, DukeMTMC-reID, CUHK03, and other medial groups, and the experimental results were 85% and 89.5%, respectively. e multimode feedback MP3 module was taken from the MP3 module in order to gain richer and denser multimode feature information. Comparing the module's initial response level (RANK1) with the various cycles yields the average accuracy for each cycle (catalog). e experiment demonstrates that the two mixed pile groups can enhance the modulus of the mixed pile group and get better results. e multi-level multi-scale pole function e ectively combines the characteristics of pedestrians in various scales, and the addition of the ASP module enhanced the network context information's overall ability to be represented, aided in this chapter's research method's ability to more thoroughly analyze scene structure, and increased the precision of pedestrian rerecognition.
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