Biological Characteristics and Genomic Analysis of Aeromonas Hydrophila Phage BUCT551 Isolated From Aquatic Sewage

Abstract

Aeromonas hydrophila is a common opportunistic pathogen in aquaculture and is ubiquitous in aquatic environment. Whereby its accessibility, variety and host specificity, phage is increasingly considered as a promising complementary medicine for antibiotics. However, a small amount of A. hydrophila phages have been characterized, which suggests the significance to isolate and characterize novel A. hydrophila phages. In this study, we isolated a novel Aeromonas hydrophila phage using A. hydrophila strain A18 as an indicator and designated it as BUCT551, and it was identified as Myoviridae phage by transmission electron microscopy (TEM). The whole genome sequencing of the phage BUCT551 revealed that it has a linear DNA genome of 613,82 bp. BLASTn analysis showed that phage BUCT551 shared 86.75% homology with A. hydrophila phage LAh_7 (Genebank ID: MK838113.1). The one-step growth curve demonstrated that phage BUCT551 had a latent period of 20 min and the burst size of 32 pfu/cell at its optimal MOI of 0.1. The phage BUCT551 had a survival pH range from 5 to 10 and tolerant temperature from 0℃ to 40℃. Host range analysis shown that the phage was able to lyse not only A. hydrophila, but also A. veronii.

The characterization and genome analysis of a novel phage phiA034 targeting multiple species of Aeromonas

“…In recent decades, with the development of high-throughput sequencing technology, whole genome sequencing for phages has become more and more popular. Taxonomy of phages based on genome alignment and phylogenetic tree analysis has been widely accepted by many virologists ( Li et al., 2022 ; Liu et al., 2022 ; Qin et al., 2021 ; Tian et al., 2021 ; Ramphul et al., 2017 ), and adopted as a guideline by the International Committee on Taxonomy of Viruses (ICTV) ( Adriaenssens and Rodney Brister, 2017 ).…”