Biological behavior of myoepithelial cells in the regeneration of rat atrophied sublingual glands following release from duct ligation

Takahashi, Shigeru; Kohgo, Takao; Nakamura, Sumiko; Arambawatta, A. K. S.; Domon, Takanori; Yamamoto, Tsuneyuki; Wakita, Minoru

doi:10.1007/s10735-005-9009-2

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…Previous studies have shown that surgical treatments induce cell proliferation in the salivary gland [15, 40, 41]. The present results, therefore, suggest that surgical resection and incision of the unilateral submandibular gland induce proliferation of MECs in residual and NC glandular tissues.…”

Section: Discussionsupporting

confidence: 72%

“…Compensatory hypertrophy has been reported in the liver following partial resection, and in the lung after unilateral excision [48]. The quantitative compensation of secretory function by proliferation of acinar cells and MECs during regeneration has also been reported [15, 40, 41]. Our recent study showed that salivary gland resection induced acinar cell differentiation from the intercalated duct in the residual tissue of the operated side, as well as the NC side in adult rats [25].…”

Section: Introductionmentioning

confidence: 95%

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Morphological Changes of Myoepithelial Cells in the Rat Submandibular Gland Following the Application of Surgical Stimuli

Kawabe

Mizobe

Bando

et al. 2016

Acta Histochemica et Cytochemica

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Myoepithelial cells (MECs) exist on the basal surface of acini in major exocrine glands, include myofilaments and various constructive proteins, and share characteristics with smooth muscle and epithelial cells. MECs project several ramified processes to invest acini, and possibly contract to compress acini to support the secretion by the glandular cells. However, the functional roles of MECs in salivary secretion are still unclear. We investigated morphological changes in immunostained MECs using the anti-α-smooth muscle actin (αSMA) antibody in operated or non-operated contralateral (NC) submandibular glands after partial or total resection. Furthermore, we investigated and discuss other salivary glands of rats. MECs in the parotid, sublingual and submandibular gland of adult rats exhibited different shapes and localizations. After surgery, in both operated and NC glands, the number of MECs and αSMA-immunopositive areas increased significantly. Three-dimensional analysis using a confocal laser-scanning microscope revealed that substantial and significant enhancement became evident in the number, length, and thickness of MEC-processes covering acini of the operated and NC submandibular glands. The preset findings indicate that MECs alter the morphology of their processes in operated and NC glands after surgery of the partial or total resection. It is suggested that MECs promote salivary secretion using elongated, thickened, and more ramified processes.

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Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 95%

Morphological Changes of Myoepithelial Cells in the Rat Submandibular Gland Following the Application of Surgical Stimuli

Kawabe

Mizobe

Bando

et al. 2016

Acta Histochemica et Cytochemica

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“…In normal submandibular glands, myoepithelial cells (containing smooth muscle actin) encompass acinar cells and are thus useful in the identification of acinar cells in ligated glands where the usual acinar characteristics have been lost. Since myoepithelial cells also surround ductal structures in atrophic glands 24, structures with an obvious lumen were excluded (Figure 4) from estimates of acinar cell number. When compared with the normal unoperated glands (Figure 4a), the 2 wk ligated glands showed an almost complete loss of acinar cells as well as an increase in staining of shrunken ducts and branch like duct structures (Figure 4b).…”

Section: Resultsmentioning

confidence: 99%

Activation of mTOR coincides with autophagy during ligation-induced atrophy in the rat submandibular gland

et al. 2010

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Salivary gland atrophy is a common consequence of pathology, including Sjögren's syndrome, irradiation therapy and obstructive sialadenitis. During severe atrophy of the rat submandibular gland caused by excretory duct ligation, the majority of acinar cells disappear through apoptosis, whereas ductal cells proliferate and dedifferentiate; yet, the gland can survive in the atrophic state almost indefinitely, with an ability to fully recover if deligated. The control mechanisms governing these observations are not well understood. We report that ∼10% of acinar cells survive in ligation-induced atrophy. Microarray and quantitative real-time PCR analysis of ligated glands indicated sustained transcription of acinar cell-specific genes, whereas ductal-specific genes were reduced to background levels. After 3 days of ligation, activation of the mammalian target of rapamycin (mTOR) pathway and autophagy occurred as shown by phosphorylation of 4E-BP1 and expression of autophagy-related proteins. These results suggest that activation of mTOR and the autophagosomal pathway are important mechanisms that may help to preserve acinar cells during atrophy of salivary glands after injury.

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“…Myoepithelial cells and progenitor cells undergo proliferation. 42 Regenerated intercalated duct cells originating from stem cells differentiated into acinar cells after release of the ligation, and myoepithelial cells were eventually located around the regenerated acinus. Therefore, myoepithelial cells are related to salivary gland regeneration.…”

Section: Discussionmentioning

confidence: 99%

The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands

2015

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This study developed an animal model of surgically wounded submandibular glands (SMGs) and investigated the effects of collagen gel with basic fibroblast growth factor (bFGF) on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF (bFGF group) or without bFGF (control group). In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day (AOD) 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, α-smooth muscle actin (αSMA), CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration.

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Biological behavior of myoepithelial cells in the regeneration of rat atrophied sublingual glands following release from duct ligation

Cited by 8 publications

References 31 publications

Morphological Changes of Myoepithelial Cells in the Rat Submandibular Gland Following the Application of Surgical Stimuli

Morphological Changes of Myoepithelial Cells in the Rat Submandibular Gland Following the Application of Surgical Stimuli

Activation of mTOR coincides with autophagy during ligation-induced atrophy in the rat submandibular gland

The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands

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