The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands

Abstract

This study developed an animal model of surgically wounded submandibular glands (SMGs) and investigated the effects of collagen gel with basic fibroblast growth factor (bFGF) on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF (bFGF group) or without bFGF (control group). In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day (AOD) 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, α-smooth muscle actin (αSMA), CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration.

Stimulation of wound healing using bioinspired hydrogels with basic fibroblast growth factor (bFGF)

“…It is well known that the bFGF can play as one of the major components in fibroblast cell proliferation in the early stage of wound healing process. 49 Therefore, the controlled burst release of bFGF from the non-crosslinking loosen section of the bioinspired hydrogels in this study could enhance the early-stage wound healing process. Moreover, the bFGF has also shown to be useful in chronic wound healing applications as well as the high-quality scar formation in the later stages of wound healing process.…”

FGF2-dependent mesenchyme and laminin-111 are niche factors in salivary gland organoids

“…3A-C; Table 1). A primary ligand for FGFR1 and FGFR2 that is known to expand many progenitor cell types is FGF2 (Kobayashi et al, 2016;Kojima et al, 2011), which is primarily expressed by the SMG mesenchyme early in development (https://sgmap.nidcr.nih.gov/) (Hoffman et al, 2002). To knockdown FGF2 in the mesenchyme, we used lentiviral infection of the primary mesenchyme to deliver Fgf2 shRNA or nontargeting shRNA control.…”

Expression of FGF‐2/FGFR‐1 in normal mucosa, salivary gland, preneoplastic, and neoplastic lesions of the oral cavity

“…In normal salivary glands, FGF‐2 is expressed in the basal membranes of intercalated ducts, acini, and basal cells of the excretory ducts (Figure ). As salivary glands present weak regenerative properties, FGF‐2 has been used to evaluate tissue regeneration of surgically wounded submandibular glands of rats, improving its healing capacity …”

“…In normal salivary glands, FGF-2 is expressed in the basal membranes of intercalated ducts, acini, and basal cells of the excretory ducts 23,24 FGF-2 has been used to evaluate tissue regeneration of surgically wounded submandibular glands of rats, improving its healing capacity. 25 Fibroblast growth factor 2 has also been administered to repair salivary gland cells damaged by radiotherapy. 26 In pleomorphic adenomas (PA), the most common salivary gland tumor (SGT), 29 FGF-2 and FGFR-1 are present in the basal membranes of the myoepithelial cells nests, around the myoepithelial cells of the myxoid areas and in the lacuna cells of the chondroid areas.…”