Biological Assay of Toxoplasma gondii Egyptian Mutton Isolates

Hassanain, Mohey A.; Elfadaly, Hassan A.; Shaapan, Raafat M.; Hassanain, Nawal A.; Barakat, Ashraf M.

doi:10.3923/ijzr.2011.330.337

Cited by 16 publications

(12 citation statements)

References 21 publications

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“…Cat bioassay was also used in some studies, and T. gondii -like oocysts were excreted from cats fed pooled meat samples. However, no further definitive procedures for theses oocysts were done in many studies (Abdel-Gawad et al ., 1984; El-Massry et al ., 1990; Hassanain et al ., 2011).…”

Section: Toxoplasmosis In Animalsmentioning

confidence: 99%

A review on toxoplasmosis in humans and animals from Egypt

Abbas

Villena

2019

Parasitology

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The present paper summarizes prevalence, epidemiology and clinical disease of natural Toxoplasma gondii infections in humans and animals from Egypt. The current situation of toxoplasmosis in Egypt is confusing. There is no central laboratory or group of researchers actively investigating toxoplasmosis in humans or animals, and no reports on the national level are available. Based on various serological tests and convenience samples, T. gondii infections appear highly prevalent in humans and animals from Egypt. Living circumstances in Egypt favour the transmission of T. gondii. Up to 95% of domestic cats, the key host of T. gondii, are infected with T. gondii; they are abundant in rural and suburban areas, spreading T. gondii oocysts. Many women have been tested in maternity clinics, most with no definitive diagnosis. Toxoplasma gondii DNA and IgM antibodies have been found in blood samples of blood donors. Clinical toxoplasmosis in humans from Egypt needs further investigations using definitive procedures. Reports on congenital toxoplasmosis are conflicting and some reports are alarming. Although there are many serological surveys for T. gondii in animals, data on clinical infections are lacking. Here, we critically review the status of toxoplasmosis in Egypt, which should be useful to biologist, public health workers, veterinarians and physicians.

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Section: Toxoplasmosis In Animalsmentioning

confidence: 99%

A review on toxoplasmosis in humans and animals from Egypt

Abbas

Villena

2019

Parasitology

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“…Our results can be compared with those in a 1995 study by Zhang et al (88.9% sensitivity and 95.7% specificity) (22), a 1990 study by Malik et al (62.5% sensitivity) (23), a 2007 study by Shaapan et al (49% sensitivity with local antigens from the RH strain of T. gondii ) (24), a 2011 study by Hassanain et al (61.4% sensitivity) (25) and a 2013 study by Al-Olayan (50% sensitivity) (26). The higher level of sensitivity in our study might be linked to our antigen preparation methods and to our region.…”

Section: Discussionmentioning

confidence: 54%

Design of Indigenous ELISA Using Tachyzoites from the RH Strain of Toxoplasma gondii and Comparison with Commercial Kits in Ahvaz, Southwest of Iran, 2015

Mohammadpour

Saki

Rafiei

et al. 2016

Jundishapur J Microbiol

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BackgroundToxoplasma gondii is one of the most common causes of latent infections in humans worldwide. Detecting anti-Toxoplasma antibodies in serum using serological tests is a common method to diagnose toxoplasmosis.ObjectivesIn the present study, an indigenous ELISA kit was prepared using tachyzoites from the RH strain of T. gondii, and its sensitivity and specificity were compared with those of commercial kits.MethodsTo produce antigens, 0.02 mL of locally isolated T. gondii RH strain parasites along with 109 tachyzoites were injected into the peritoneal cavities of 50 laboratory mice (BALB/C). Parasites were collected after 4 days. After filtering and washing, the concentration of protein in sonicated tachyzoites was calculated using the Lowry protein assay. The dilution of antigen, serum and alkaline phosphatase conjugate was assessed in designing an indigenous ELISA method; then ELISA was performed based on these dilutions, and its sensitivity was determined using 200 serum samples. In addition, the specificity of the assay was evaluated using 40 serum samples from patients with tuberculosis, leukemia or hydatid cyst.ResultsIndigenous ELISA was used to examine 100 serum samples containing anti-T. gondii IgG, with a sensitivity of 98% (commercial kits: 100%). Another 100 serum samples containing anti-T. gondii IgM were also tested, with a sensitivity of 99% (commercial kits: 100%). When 40 serum samples from patients with leukemia, hydatid cyst or tuberculosis were examined using anti-T. gondii IgG, the specificity was 100%, identical to commercial kits. However, the specificity of a similar test with anti-T. gondii IgM was just 28.6% for serum samples from leukemia patients, 21.4% for hydatid cyst and 16.7% for tuberculosis.ConclusionsWe found that purified locally isolated soluble crude antigens of the RH strain of T. gondii from the peritoneal cavity of mice may be one of the most promising antigens for detection of human toxoplasmosis in routine screening.

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“…This observation is probably due to the presence of more common components in TgLA with FgA than other antigens. Moreover, TgLA is a virulent Egyptian mutton isolates (Shaapan and Khalil 2008;Hassanain et al 2011;Dubey et al 2012) while, RH strain and ME49 are isolates significantly more associated with human congenital toxoplasmosis than with animal infection (Howe and Sibley 1995).…”

Section: Discussionmentioning

confidence: 99%

Significance of a common 65 kDa antigen in the experimental fasciolosis and toxoplasmosis

Shaapan

Toaleb²,

Abdel-Rahman³

2013

J Parasit Dis

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In the current study, cross-reaction between two important zoonotic parasites; extracellular helminthes Fasciola gigantica and intracellular protozoa Toxoplasma gondii was proved by enzyme linked immunosorbent assay. Five antigens were used to identify and compare the cross-binding activities in the prepared antisera. Two F. gigantica antigens; adult flukes (FgA) and eggs (FgEA) were used to detect IgG in T. gondii naturally infected human sera (TgIHS) and experimentally infected sera of sheep (TgISS), mice (TgIMS) and rats (TgIRS). Three types of T. gondii antigens; RH (TgRHA), local sheep isolate (TgLA) and ME49 isolate (TgMEA) were used to detect cross binding activities in F. gigantica experimental infected rabbit sera (FgIRS) and F. gigantica naturally infected bovine sera (FgIBS). The cross-binding activities in the prepared antisera were strongly directed towards FgA and TgLA rather than the other antigens. The characterization of the five antigens using SDS-PAGE showed 4 common bands of FgA and TgLA; 165, 97, 76, and 65 kDa. While two common bands were observed between TgRHA, TgMEA and FgA; 165, and 65 kDa. Whereas, two common bands found between three types of T. gondii antigens and FgEA were identified; 165 and 65 kDa. The immunogenic cross-reactive bands between FgA and TgLA with F. gigantica infected bovine sera were identified by immunoblot. In FgA, the common immunogenic bands were 165, 65 and 14 kDa. While in TgLA, common immunogenic bands were 165 and 65 kDa. Whereas, the common immunogenic band between FgA and TgLA identified with T. gondii experimentally infected sheep sera was 65 kDa. The current research proves cross reaction between F. gigantica and T. gondii. One common band of 65 kDa showed broad immunogenic cross-reactivity with the developed antisera raising the prospect of being putative common immunodiagnostic candidate of both infections.

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Biological Assay of Toxoplasma gondii Egyptian Mutton Isolates

Cited by 16 publications

References 21 publications

A review on toxoplasmosis in humans and animals from Egypt

A review on toxoplasmosis in humans and animals from Egypt

Design of Indigenous ELISA Using Tachyzoites from the RH Strain of Toxoplasma gondii and Comparison with Commercial Kits in Ahvaz, Southwest of Iran, 2015

Significance of a common 65 kDa antigen in the experimental fasciolosis and toxoplasmosis

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