Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation

Abstract

21In this work, we aimed at the production of bovine sodium caseinate (NaCAS) hydrolysates 22 by means of an extracellular protease from Bacillus sp. P7. Mass spectrometry was carried 23 out to evaluate peptide mass distribution and identified sequences of peptides with a 24 signal/noise ratio higher than 10. Antioxidant and antimicrobial properties of hydrolysates 25 were evaluated. An acid-induced aggregation process of the hydrolysates and their 26 corresponding mixtures with NaCAS were also analyzed. The results showed that the 27 enzymatic hydrolysis produced peptides, mostly lower than 3 kDa, with different 28 bioactivities depending on the time of hydrolysis (ti). These hydrolysates lost their ability to 29 aggregate by addition of glucono--lactone, and their incorporation into NaCAS solutions 30 alter the kinetics of the process. Also, the degree of compactness of the NaCAS aggregates, 31 estimated by the fractal dimension of aggregates, was not significantly altered by the 32 incorporation of hydrolysates. However, at higher protein concentrations, when the 33 decrease in pH leads to the formation of NaCAS acid gels, the presence of hydrolysates 34 alters the microstructure and rheological behavior of these gels. 35 36

Production and Characterization of Antioxidative Hydrolysates and Peptides from Corn Gluten Meal Using Papain, Ficin, and Bromelain

“…Enzymatic hydrolysis was reported to benefit DPPH scavenging activity of other food proteins, such as milk protein [ 24 ], wheat germ protein [ 25 ], and rice protein [ 26 ]. Hidalgo et al [ 27 ] found that DPPH scavenging of bovine sodium caseinate hydrolysates, which obtained by bacterial proteases produced from Bacillus sp. P7, tended to increase with hydrolysis time, while this study showed no clear correlation between hydrolysis time and DPPH scavenging activity for all the hydrolysates from the three enzymes.…”

Milk protein suspensions enriched with three essential minerals: Physicochemical characterization and aggregation induced by a novel enzymatic pool

“…isolated from the intestine of the Amazon basin fish, Piaractus mesopotamicus, was characterized and results of inhibition studies and zymogram analysis suggested that P7 consist primarily of serine proteases [5]. The bioactivity of caseinate hydrolysates obtained with P7 has also been investigated [6,7] and P7 has been shown to exhibit milk clotting activity [8].…”

Iron-caseinglycomacropeptide complexes: Characterization and application in beverages

“…The ferrozine method (Chang et al, 2007;Hidalgo et al, 2015;Oloughlin, Kelly, Murray, Fitzgerald, & Brodkorb, 2015;Smialowska, Matia-Merino, & Carr, 2017) has been widely used for estimating the binding ability of peptides by determining the unbound or free iron that can be detected just when the maximum load of iron is reached. Figure 1 shows the free iron occurrence when increasing the molar Fe/CMP ratio from 1.1 to 8.0 at pH 6.5.…”