Biological and antibacterial properties of the micro-nanostructured hydroxyapatite/chitosan coating on titanium

Abstract

Titanium (Ti) is the widely used implant material in clinic, however, failures still frequently occur due to its bioinertness and poor antibacterial property. To improve the biological and antibacterial properties of Ti implants, micro-nanostructured hydroxyapatite (HA) coating was prepared on Ti surface by micro-arc oxidation (MAO), and then the antibacterial agent of chitosan (CS) was loaded on the HA surface through dip-coating method. The results showed that the obtained HA/CS composite coating accelerated the formation of apatite layer in SBF solution, enhanced cell adhesion, spreading and proliferation, and it also inhibited the bacterial growth, showing improved biological and antibacterial properties. Although, with the increased CS amount, the coverage of HA coating would be enlarged, resulting in depressed biological property, however, the antibacterial property of the composite coating was enhanced, and the cytotoxicity about CS was not detected in this work. In conclusion, the HA/CS coating has promising application in orthopedics, dentistry and other biomedical devices.

In-Situ Synthesis and Characterization of Chitosan/Hydroxyapatite Nanocomposite Coatings to Improve the Bioactive Properties of Ti6Al4V Substrates

“…The surface roughness is a well-known factor that determines the biological properties such as apatite layer deposition in SBF and cellular responses [ 23 ]. Based on the clinical results from retrieved implants, rough surface of implants can improve the ingrowth of soft and hard tissue into the materials, thereby making more biological anchorage to enhance the stability of the Ti-base implant [ 55 ].…”

“…Similarly, HA/CS composite coatings with higher HA content prepared by micro-arc oxidation and dip-coating methods presented a porous micro-nano structure. As the loaded CS amount was increased, the antibacterial property enhanced [ 23 ]. However, the antibacterial properties may be improved by loading of specific compounds (i.e., antibiotics, antimicrobial peptides, etc) in order to obtain a local release able to inhibit bacteria growth and prevent the biofilm formation during in vivo implantation [ 24 ].…”

Therapeutics and delivery vehicles for local treatment of osteomyelitis

“…In recent studies, antibiotics, phages, and lysostaphin released from films/coatings made of phosphatidylcholine, 137,138 hydroxypropylmethlycellulose (HPMC), 35 poly(D,L-lactide) (PDLLA), 139 and a hyaluronic acid/poly-lactic acid hydrogel (DAC) 111 have been explored, among others. 97,[140][141][142][143][144][145][146] Interestingly, silver as a coating material may be used as both an antimicrobial and a delivery vehicle, or combined with other materials such as zeolites (made of aluminum and silicon), which are beneficial in this pairing because of their crystal-like porous structure. 97 In one study, zeolite alone decreased bacterial counts by approximately 3000 units.…”

Chitosan–Hydroxyapatite Composite Layers Generated in Radio Frequency Magnetron Sputtering Discharge: From Plasma to Structural and Morphological Analysis of Layers

“…Chitosan-hydroxyapatite composite coatings have been obtained, until now, by: immersion [6], micro-arc oxidation [7], sol gel [8], biomimetic or electrophoretic deposition methods [9]. However, magnetron sputtering, thermal plasma spraying, pulsed laser deposition or electrospinning methods are extensively used for calcium phosphate-based coating depositions on polymeric or metallic substrates [1][2][3].…”