Biological agent detection with the use of an airborne biosensor

Anderson, George P.; King, Keeley D.; Cuttino, David S.; Whelan, James P.; Ligler, Frances S.; MacKrell, Joseph F.; Bovais, Christopher; Indyke, David K.; Foch, Richard J.

doi:10.1002/(sici)1520-6521(1999)3:4/5<307::aid-fact9>3.0.co;2-m

Cited by 40 publications

(26 citation statements)

References 4 publications

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“…This method of immobilization has been shown to yield surfaces with improved functionality when compared to surfaces with antibodies immobilized via direct covalent linkage [34,35]. Antibodies were biotinylated using a fivefold molar excess of biotin-LC-NHS ester (Pierce, Rockland, IL, USA).…”

Section: Antibody Preparationmentioning

confidence: 99%

Array biosensor for detection of toxins

Ligler

Taitt

Shriver‐Lake

et al. 2003

Analytical and Bioanalytical Chemistry

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The array biosensor is capable of detecting multiple targets rapidly and simultaneously on the surface of a single waveguide. Sandwich and competitive fluoroimmunoassays have been developed to detect high and low molecular weight toxins, respectively, in complex samples. Recognition molecules (usually antibodies) were first immobilized in specific locations on the waveguide and the resultant patterned array was used to interrogate up to 12 different samples for the presence of multiple different analytes. Upon binding of a fluorescent analyte or fluorescent immunocomplex, the pattern of fluorescent spots was detected using a CCD camera. Automated image analysis was used to determine a mean fluorescence value for each assay spot and to subtract the local background signal. The location of the spot and its mean fluorescence value were used to determine the toxin identity and concentration. Toxins were measured in clinical fluids, environmental samples and foods, with minimal sample preparation. Results are shown for rapid analyses of staphylococcal enterotoxin B, ricin, cholera toxin, botulinum toxoids, trinitrotoluene, and the mycotoxin fumonisin. Toxins were detected at levels as low as 0.5 ng mL(-1).

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Section: Antibody Preparationmentioning

confidence: 99%

Array biosensor for detection of toxins

Ligler

Taitt

Shriver‐Lake

et al. 2003

Analytical and Bioanalytical Chemistry

Self Cite

270

133

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“…Previous research has shown the value of manned and unmanned systems for aerobiological sampling (Anderson et al, 1999;Corrigan, Roberts, Ramana, Kim, & Ramanathan, 2008;Dingus, 2008;Ingold, 1939;Peräjärvi, Lehtinen, Pöllänen, & Toivonen, 2008;Schmale, Dingus, & Reinholtz, 2008;Techy, Schmale, & Woolsey, 2010;Techy, Woolsey, & Schmale, 2008). Dynamic sampling systems have the potential to improve upon current static, groundbased sampling methods.…”

Section: Introductionmentioning

confidence: 99%

“…Thus it cannot illustrate a multiple sampling as a function of time in different geographic areas. In other research, Anderson et al (1999) used a multichannel fluorimeter and a custom ram-air-driven cyclone particle collector for air sampling experiments. Purified polycyclonal rabbit antibodies were fluoresced using fluorescent dye and dispersed in a predefined test area.…”

Section: Introductionmentioning

confidence: 99%

Development of an autonomous unmanned aerial system to collect time‐stamped samples from the atmosphere and localize potential pathogen sources

Gonzalez

Castro

Narayan

et al. 2011

Journal of Field Robotics

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This paper presents the hardware development and testing of a new concept for air sampling via the integration of a prototype spore trap onboard an unmanned aerial system (UAS). We propose the integration of a prototype spore trap onboard a UAS to allow multiple capture of spores of pathogens in single remote locations at high or low altitude, otherwise not possible with stationary sampling devices. We also demonstrate the capability of this system for the capture of multiple time-stamped samples during a single mission. Wind tunnel testing was followed by simulation, and flight testing was conducted to measure and quantify the spread during simulated airborne air sampling operations. During autonomous operations, the onboard autopilot commands the servo to rotate the sampling device to a new indexed location once the UAS vehicle reaches the predefined waypoint or set of waypoints (which represents the region of interest). Time-stamped UAS data are continuously logged during the flight to assist with analysis of the particles collected. Testing and validation of the autopilot and spore trap integration, functionality, and performance is described. These tools may enhance the ability to detect new incursions of spores. C 2011 Wiley Periodicals, Inc.

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“…Rowe-Taitt et al detected B. anthracis, F. tularensis, B. abortus with NRL array biosensor in 2000 [17]. Anderson et al investigated B. globigii with Analyte 2000 biosensor in 1999 [6], and then continued to detect B. anthracis, S. typhimurium, Giardia, F. tularensis with biosensor RAPTOR in recent years [1,5,7,8]. Cao et al detected Y. pestis antigen FI and analyzed it in serum, whole blood and plasma and obtained accuracy for determining antigen concentrations from 50 to 400 ng/ml with a fiber optic biosensor in 1995 [11].…”

Section: Introductionmentioning

confidence: 99%