Biological Activity of Sea Bass (Dicentrarchus labrax L.) Recombinant Interleukin-1β

Abstract

Biological activities of a putative mature sea bass interleukin-1beta peptide, produced as a recombinant protein (rIL-1beta) in Escherichia coli, have been investigated. The rIL-1beta contains a 6-histidine tag at the N-terminus, and protein purification has been achieved through this tag by affinity chromatography. Biological activities have been investigated both at the cellular and gene expression levels. In in vitro assays sea bass rIL-1beta induced the proliferation of murine D10.G4.1 cells and increased yeast phagocytosis by sea bass head kidney leukocytes. The purified cytokine was also tested in a lymphocyte-activation factor assay, where it induced the proliferation of sea bass thymocytes. Finally, in an in vivo assay, rIL-1beta administered intraperitoneally increased expression levels of the IL-1beta gene and activated macrophages to produce a cyclooxygenase 2 homologue (COX-2) gene in the head kidney.

A Review of Immune System Components, Cytokines, and Immunostimulants in Cultured Finfish Species

“…Various cytokines are employed to transmit directions to macrophages and direct effective phagocytosis. It has been hypothesized that IL-1β (Interleukin 1 beta) is responsible for phagocyte recruitment through the activation of chemoattractants and enhanced immune gene expression [21]. Additionally, macrophages also harness the ability to produce transferrin, a serum protein that is capable to binding excess iron from the plasma, in an effort to limit bacterial metabolism [20].…”

Inflammatory Effects of Edwardsiella ictaluri Lipopolysaccharide Modifications in Catfish Gut

“…Also, IL-1␤ has the potential to enhance the immune response induced by vaccines (63), and recombinant IL-1␤ has been use as an immunostimulant for vaccines in sheep (79,80), pigs (81), cattle (82), and sea bass (83,84). In teleosts, the administration of immunostimulants, such as ␤-1,3-glucan and peptidoglycan, helps to prevent infections through activation of phagocytes, such as neutrophils, monocytes, and macrophages, suggesting that the activation of innate immunity in teleost fish by immunostimulants is a useful method of disease prevention that can replace the use of antibiotics.…”

Characterization of two grouper Epinephelus akaara cell lines: Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus–host interaction

“…Continuous cell lines could provide an important tool for studying epidemiology, toxicology, cellular physiology and the host-pathogen interactions Hong et al, 2004;Buonocore et al, 2005). The establishment of healthy fish cell lines is essential for isolation, identification and characterization of infectious viruses from fishes (Parameswaran et al, 2007;Dong et al, 2008).…”