“…In addition, for any of the mentioned endemics there are no data about potential application as a source of different antimicrobial and antitumor agents. Since the plants from Hieracium and Onosma genera are a well-known source of different antioxidants, [4,10,19,[11][12][13][14][15][16][17][18] enzyme inhibitors, [11,13 -20] anti-diabetic, [16,21] antiproliferative [10] antimicrobial [2,10] as well as antitumor [21,22] agents, the current study aimed to achieve the following: a) to determine a general phytochemical profile of above-ground parts (stem, leaf and flower) of H. waldsteinii and O. stellulata by determining the total phenolic, flavonoid and pigment (carotenoid, chlorophyll A and B) content as well as the content of soluble sugars and starch; b) to determine a detailed phenolic profile of the above-mentioned endemic species via HPLC MS-MS analysis; c) to determine antioxidant properties using four different assays including DPPH * and ABTS * + scavenging tests as well as in vitro phosphomolybdenum total antioxidant capacity (TAC) and ferric reducing power (FRP) tests; d) to evaluate possible antimicrobial activity of examined extracts against several bacteria and fungi; e) to approach possible application of extracted H. waldsteinii and O. stellulata bioactive compounds as antitumor agents.…”