BIOLOGICAL ACTIVITY OF METHANOL EXTRACT OF Elaeocarpus mastersii KING: ANTIOXIDANT, ANTIBACTERIAL, AND α-GLUCOSIDASE INHIBITOR

Abstract

Plants are one of the natural resources that have great biological activity and have long been used in traditional medicine. In this research, methanol extracts of root, stem bark, and leaf of Elaeocarpus mastersii King were tested to determine phenolic and flavonoid content and evaluate their biological activities as the antioxidant, antibacterial, and antidiabetic using the method of DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, disc diffusion against Staphylococcus epidermidis (S. epidermidis), Staphylococcus aureus (S. aureus), Salmonella typhosa (S. typhosa), and Escherichia coli (E. coli), and inhibition of α-glucosidase enzyme, respectively. All parts showed high phenolic content and antioxidant activity wherein the leaf extract was found to be the highest value (340.36 ± 2.09 mg GAE/g DW and IC 50 1.86 ± 0.00 µg/mL) followed by the stem bark (331.53 ± 6.96 mg GAE/g DW and IC 50 2.43 ± 0.01 µg/mL) and the root (216.27 ± 3.19 mg GAE/g DW and IC 50 3.37 ± 0.20 µg/mL), respectively. In contrast, their flavonoid values were very low. The stem bark exhibited the highest antibacterial activity against all of the tested bacteria. The activity of α-glucosidase inhibition revealed that the stem bark had the highest activity among all parts with the IC 50 of 14.56 ± 1.20 µg/mL. This study demonstrates that Elaeocarpus mastersii King has a great biological activity as the antioxidant, antibacterial, and α-glucosidase inhibitor.

Phytochemical Screening, Antioxidant, Antidiabetic and Anticancer Activities of Elaeocarpus variabilis Fruit

“…Therefore, inhibition of α-glucosidase will be one of the most effective approaches to control diabetes (Sheikh et al, 2008). Elaeocarpus genus is used as folk medicines for diabetics and hypertensive disease (Okselni et al, 2019). Talukdar et al (2016) state that in vitro anti-diabetic activity of E. ganitrus bark extract has an inhibitory effect on the α-amylase enzyme which may be used to control high blood glucose levels.…”

BIOACTIVE COMPOUND FROM THE MANGROVE PLANT ENDOPHYTIC FUNGUS Diaporthe amygdali SgKB4

“…Antibacterial activity was detected after incubation for 24 h at 30 o C. A clear zone of inhibition in the media was considered as an indicator of antibacterial activity. 11,12,13…”

Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leaves

“…Elaeocarpus mastersii King belongs to Elaeocarpus genus that spread widely in Indonesia. This plant has high total phenolic contents [16,17]. Unfortunately, the literature studies showed that the isolated compounds of E. mastersii King were limited to the compounds of anthocyanins, cucurbitacins, ellagic acid derivatives, β-sitosterol, and βsitosterol glucoside [18][19][20].…”