Biological activity of laminin/polylaminin-coated poly-ℇ-caprolactone filaments on the regeneration and tissue replacement of the rat sciatic nerve

Siqueira-Santos, R. de; Sardella-Silva, Gabriela; Nascimento, Marcos Assis; Oliveira, Leandro T.; Coelho‐Sampaio, Tatiana; Ribeiro-Resende, Victor Túlio

doi:10.1016/j.mtbio.2019.100026

Cited by 5 publications

(8 citation statements)

References 39 publications

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“…Comparing cell growth between the scaffold with only plasma treatment and the scaffold with PDL + LM single coating after plasma treatment revealed that more cells adhered and survived on the latter two scaffolds, indicating that these two coatings provide a more favorable microenvironment to promote the growth of PC12 cells. [ 69–73 ] PC12 cells showed the best proliferative behavior for the scaffold with PDL and LM double coatings after plasma treatment, indicating that this kind of modification was the most effective for PC12 cell proliferation.…”

Section: Resultsmentioning

confidence: 99%

Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

Chen

Jiang

Zhu

et al. 2021

Macromolecular Bioscience

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Tissue engineering scaffolds provide an encouraging alternative for nerve injuries due to their biological support for nerve cell growth, which can be used for neuronal repair. Nerve cells have been reported to be mostly cultured on 2D scaffolds that cannot mimic the native extracellular matrix. Herein, highly ordered 3D scaffolds are fabricated for nerve cell culture by melt electrospinning writing, the microstructures and geometries of the scaffolds could be well modulated. An effective strategy for scaffold surface modification to promote nerve cell growth is proposed. The effects of scaffolds with different surface modifications, viz., plasma treatment, single poly-D-lysine (PDL) coating after plasma treatment, single laminin (LM) coating after plasma treatment, double PDL and LM coatings after plasma treatment, on PC12 cell growth are evaluated. Experiments show the scaffold modified with double PDL and LM coatings after plasma treatment facilitated the growth of PC12 cells most effectively, indicating the synergistic effect of PDL and LM on the growth of nerve cells. This is the first systematic and quantitative study of the effects of different scaffold surface modifications on nerve cell growth. The above results provide a versatile culture platform for growing nerve cells, and for recovery from peripheral nerve injury.

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Section: Resultsmentioning

confidence: 99%

Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

Chen

Jiang

Zhu

et al. 2021

Macromolecular Bioscience

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Section: Discussionmentioning

confidence: 99%

“…In the field of peripheral nerve regeneration, neurite outgrowth of DRG neurons (DRGs) is a main indicator to predict peripheral axonal regrowth after injury. de Siqueira-Santos et al [27] suggested that extending sensory fibers of DRGs in vitro might be a regenerative property for promoting functional recovery after SNI, and Bucan et al [28] reported that DRG neurite elongation activated by exosomes forms adipose derived mesenchymal stem cells closely involved in peripheral nerve regeneration. In the present study, nobiletin dose-dependently regulated neurite outgrowth of primary cultured DRGs after SNI and DRG neurite elongation was significantly facilitated at 50 and 100 µM of nobiletin compared to other dosages.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Effects of Nobiletin on DRG Neurite Elongation and Axon Growth after Sciatic Nerve Injury

Seo

Jeon

Kim³

et al. 2021

IJERPH

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Sciatic nerve injury (SNI) leads to sensory and motor dysfunctions. Nobiletin is a major component of polymethoxylated flavonoid extracted from citrus fruits. The role of nobiletin on sciatic nerve regeneration is still unclear. Thus, the purpose of this study was to investigate whether nobiletin increases DRG neurite elongation and regeneration-related protein expression after SNI. Cytotoxicity of nobiletin was measured in a concentration–dependent manner using the MTT assay. For an in vitro primary cell culture, the sciatic nerve on the middle thigh was crushed by holding twice with forceps. Dorsal root ganglion (DRG) and Schwann cells were cultured 3 days after SNI and harvested 36 h later and 3 days later, respectively. In order to evaluate specific regeneration-related markers and axon growth in the injured sciatic nerve, we applied immunofluorescence staining and Western blot techniques. Nobiletin increased cell viability in human neuroblastoma cells and inhibited cytotoxicity induced by exposure to H2O2. Mean neurite length of DRG neurons was significantly increased in the nobiletin group at a dose of 50 and 100 μM compared to those at other concentrations. GAP-43, a specific marker for axonal regeneration, was enhanced in injury preconditioned Schwann cells with nobiletin treatment and nobiletin significantly upregulated it in injured sciatic nerve at only 3 days post crush (dpc). In addition, nobiletin dramatically facilitated axonal regrowth via activation of the BDNF-ERK1/2 and AKT pathways. These results should provide evidence to distinguish more accurately the biochemical mechanisms regarding nobiletin-activated sciatic nerve regeneration.

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“…A nerve conduit should ideally promote the proliferation of Schwann cells and fibroblasts in the nerve connective tissue to form Büngner bands for axonal regeneration [3]. For this purpose, the development of porous nerve conduits is an essential feature for the regeneration of nerve tissue as the pore structure prevents the infiltration of scar tissue while providing nutrients.…”

Section: Fabrication Of the Cepp Monolithmentioning

confidence: 99%

“…Peripheral nerve injuries, usually caused by traumatic lesions or tumor expiration, are always accompanied by dysfunction, movement and sensory disorders, and neuropathic pains, which seriously influence the patient's quality of life [1,2]. Although peripheral nerves have an intrinsic potential to regenerate after lesions, damage involving extensive nerve tissue loss precludes the reconnection between proximal and distal nerve stumps [3]. Autologous transplantation is the main clinical intervention for repairing peripheral nerve injury.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration

Lee

Kim

Kwon

et al. 2022

IJMS

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The development of nerve conduits with a three-dimensional porous structure has attracted great attention as they closely mimic the major features of the natural extracellular matrix of the nerve tissue. As low levels of reactive oxygen species (ROS) function as signaling molecules to promote cell proliferation and growth, this study aimed to fabricate protoporphyrin IX (PpIX)-immobilized cellulose (CEPP) monoliths as a means to both guide and stimulate nerve regeneration. CEPP monoliths can be fabricated via a simple thermally induced phase separation method and surface modification. The improved nerve tissue regeneration of CEPP monoliths was achieved by the activation of mitogen-activated protein kinases, such as extracellular signal-regulated kinases (ERKs). The resulting CEPP monoliths exhibited interconnected microporous structures and uniform morphology. The results of in vitro bioactivity assays demonstrated that the CEPP monoliths with under 0.54 ± 0.07 μmol/g PpIX exhibited enhanced photodynamic activity on Schwann cells via the generation of low levels of ROS. This photodynamic activation of the CEPP monoliths is a cell-safe process to stimulate cell proliferation without cytotoxic side effects. In addition, the protein expression of phospho-ERK increased considerably after the laser irradiation on the CEPP monoliths with low content of PpIX. Therefore, the CEPP monoliths have a potential application in nerve tissue regeneration as new nerve conduits.

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Biological activity of laminin/polylaminin-coated poly-ℇ-caprolactone filaments on the regeneration and tissue replacement of the rat sciatic nerve

Cited by 5 publications

References 39 publications

Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

In Vitro and In Vivo Effects of Nobiletin on DRG Neurite Elongation and Axon Growth after Sciatic Nerve Injury

Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration

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