Biological Activity of Hydroxo-vitamin B<sub>12</sub> Degradation Product Formed during Microwave Heating

Watanabe, Fumio; Abe, K.; Katsura, Hiromi; Takenaka, Shigeo; Mazumder, Sraboni; Yamaji, Ryoichi; Ebara, Syuhei; Fujita, Tomoyuki; Tanimori, Shinji; Kirihata, and Mitsunori; Nakano, Yoshihisa

doi:10.1021/jf980727v

Cited by 24 publications

(15 citation statements)

References 7 publications

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“…For analysis for vitamin B12, acetate buffer and potassium cyanide were added to urine, and vitamin B12 in urine was converted to cyanocobalamin by autoclave (22). Urinary content of cyanocobalamin was determined by the microbioassay method using Lactobacillus leichmannii, ATCC 7830 (22).…”

Section: Methodsmentioning

confidence: 99%

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Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan

Shibata

Fukuwatari

Sasaki

et al. 2013

J Nutr Sci Vitaminol

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Dietary habits of pregnant and lactating women have a critical impact on the health of future infants. For this reason, the Japanese Dietary Reference Intakes regarding the amounts of addition of water-soluble vitamins have been set (1). The additional intake amounts for pregnant and lactating women were calculated based on data concerning the changes in the metabolic characteristics of vitamins in pregnant women and the loss of vitamins as a result of lactation. However, there is little information about whether nutritional statuses of water-soluble-vitamins in Japanese pregnant and lactating women are good or not. Therefore, we examined the nutritional status of pregnant and lactating women and speculated about the validity of the additional amount of some water-soluble vitamins in the dietary reference intakes for pregnant and lactating women.A potential approach for determining the validity for the additional amounts is based on the observation that a water-soluble vitamin or its catabolite(s) can be detected in the urine (2-11). Using this approach, the urinary excretion of a water-soluble vitamin or its catabolite(s) occurs when the dietary intake exceeds an individual necessary amount. MATERIALS AND METHODSSubjects. Japanese pregnant (16-40 wk gestation) or lactating women (0-11 mo postpartum) were recruited from a parenting circle in The University of Shiga Prefecture between April 2010 and February 2012. Japanese married non-pregnant, non-lactating women also were recruited as controls through the parenting circle to match the mean ages and heights of the pregnant and lactating women. The purpose and protocol of this study was explained to all participants before joining the study, and written informed consent was obtained from each participant. We excluded participants diagnosed with a cold or influenza, and those who had taken multi-vitamin supplements at least once during the previous month. In addition, we excluded participants whose 24-h urine collection was considered incomplete: a collection time outside the 22-26-h range, urine volume ,250 mL, or extremely low or high energy intake (,500 or .4,000 kcal/d) (12). Twenty-four of the 2nd trimester pregnant (16-27 wk gestation) and 32 of the Bunkyo-ku, Tokyo 113-0033, Japan (Received December 17, 2012) Summary Recent studies have shown that the urinary excretion levels of water-soluble vitamins can be used as biomarkers for the nutritional status of these vitamins. To determine changes in the urinary excretion levels of water-soluble vitamins during pregnant and lactating stages, we surveyed and compared levels of nine water-soluble vitamins in control (non-pregnant and non-lactating women), pregnant and lactating women. Control women (n537), women in the 2nd (16-27 wk, n524) and 3rd trimester of pregnancy (over 28 wk, n532), and early-(0-5 mo, n554) and late-stage lactating (6-11 mo, n549) women took part in the survey. The mean age of subjects was ~30 y, and mean height was ~160 cm. A single 24-h urine sample was collected 1 d after the comple...

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Section: Methodsmentioning

confidence: 99%

“…Urinary content of cyanocobalamin was determined by the microbioassay method using Lactobacillus leichmannii, ATCC 7830 (22). For analysis of MNA, 2-Py and 4-Py, nicotinamide metabolites, 1 mL of 1 mol/L HCl was added to 9 mL of urine.…”

Section: Methodsmentioning

confidence: 99%

Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan

Shibata

Fukuwatari

Sasaki

et al. 2013

J Nutr Sci Vitaminol

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“…Prolonged heat-treatments (boiling for 30 min or microwaving for 6 min) of authentic OH-B12 solution have demonstrated the accelerated formation of B12 degradation products, some of which are identified as the compounds with various changes to lower-ligand moiety (cobalt-coordinated nucleotide) (9,17).…”

Section: Effects Of Various Cooking Methods On B12 Contents Of the Ramentioning

confidence: 99%

Loss of Vitamin B12 in Fish (Round Herring) Meats during Various Cooking Treatments

Nishioka

Kanosue

Yabuta

et al. 2011

J Nutr Sci Vitaminol

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SummaryThe loss of vitamin B 12 in round herring meats during various cooking treatments was evaluated. Although amounts of vitamin B 12 were three times greater in the viscera (37.5 Ϯ 10.6 g/100 g fresh weight) than in the meats, about 73% of total vitamin B 12 found in the whole fish body (except for head and bones) were recovered in the meats (5.1 Ϯ 1.0 g of vitamin B 12 ). The vitamin B 12 contents of the round herring's meats were significantly decreased up to ~62% during cooking by grilling, boiling, frying, steaming, and microwaving. There was, however, no loss of vitamin B 12 during vacuum-packed pouch cooking. Model experiment using hydroxocobalamin suggest that loss of vitamin B 12 is dependent on the degree of temperature and time used in conventional cooking, and is further affected by the concomitant ingredients of food. Retention of vitamin B 12 was not dependent on vacuum or temperature (or both) used in the vacuum-packed pouch cooking.

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“…lactis ATCC 7830. 22) Niacin: One milliliter of 1 M HCl was added to 9 ml of the 24-h urine sample. The quantity of Nam, 2-Py and 4-Py in the urine was measured simultaneously by the HPLC method of Shibata et al…”

Section: Methodsmentioning

confidence: 99%

Effects of Excess Biotin Administration on the Growth and Urinary Excretion of Water-Soluble Vitamins in Young Rats

Sawamura

Fukuwatari

Shibata

2007

Bioscience, Biotechnology, and Biochemistry

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Biological Activity of Hydroxo-vitamin B₁₂ Degradation Product Formed during Microwave Heating

Cited by 24 publications

References 7 publications

Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan

Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan

Loss of Vitamin B12 in Fish (Round Herring) Meats during Various Cooking Treatments

Effects of Excess Biotin Administration on the Growth and Urinary Excretion of Water-Soluble Vitamins in Young Rats

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Biological Activity of Hydroxo-vitamin B12 Degradation Product Formed during Microwave Heating

Cited by 24 publications

References 7 publications

Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan

Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan

Loss of Vitamin B12 in Fish (Round Herring) Meats during Various Cooking Treatments

Effects of Excess Biotin Administration on the Growth and Urinary Excretion of Water-Soluble Vitamins in Young Rats

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Product

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Biological Activity of Hydroxo-vitamin B₁₂ Degradation Product Formed during Microwave Heating