Effects of Excess Biotin Administration on the Growth and Urinary Excretion of Water-Soluble Vitamins in Young Rats

Sawamura, Hiromi; Fukuwatari, Tsutomu; Shibata, Katsumi

doi:10.1271/bbb.70381

Cited by 22 publications

(15 citation statements)

References 31 publications

(26 reference statements)

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“…However, the biotin-supplemented group showed reduced food intake, impaired body weight gain, and decreased kidney and liver weights. It is possible that the histological modifications might result from biotin toxic effects because the magnitude of biotin intake is above the reported tolerable upper intake level found by the same investigators [35]. …”

Section: Discussionmentioning

confidence: 97%

Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Riverón-Negrete

Sicilia-Argumedo

Álvarez-Delgado

et al. 2016

BioMed Research International

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Pharmacological concentrations of biotin have pleiotropic effects. Several reports have documented that biotin supplementation decreases hyperglycemia. We have shown that a biotin-supplemented diet increased insulin secretion and the mRNA abundance of proteins regulating insulin transcription and secretion. We also found enlarged pancreatic islets and modified islet morphology. Other studies have shown that pharmacological concentrations of biotin modify tissue structure. Although biotin administration is considered safe, little attention has been given to its effect on tissue structure. In this study, we investigated the effect of biotin supplementation on hepatic morphology and liver toxicity markers. Male BALB/cAnN Hsd mice were fed a control or a biotin-supplemented diet for 8 weeks. Versus the control mice, biotin-supplemented mice had an altered portal triad with dilated sinusoids, increased vascularity, and bile conducts. Furthermore, we observed an increased proportion of nucleomegaly and binucleated hepatocytes. In spite of the liver morphological changes, no differences were observed in the serum liver damage indicators, oxidative stress markers, or antioxidant enzymes. Our data demonstrate for the first time that biotin supplementation affects liver morphology in normal mice, and that these modifications are not paralleled with damage markers.

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Section: Discussionmentioning

confidence: 97%

Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Riverón-Negrete

Sicilia-Argumedo

Álvarez-Delgado

et al. 2016

BioMed Research International

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“…We previously reported that a 1.0% biotin diet affected the growth of young mice (Sawamura et al. ). Based on the findings of these studies, we speculated that high doses of biotin may affect reproduction in males.…”

Section: Discussionmentioning

confidence: 99%

“…We previously reported that the oral administration of 1 g biotin/kg diet to pregnant mice during gestation had no effect on the rate of successful pregnancies (Watanabe 1996). Furthermore, we recently demonstrated that some rats fed a 0.8% (8 g biotin/kg diet) or higher biotin diet had diarrhea and died (Sawamura et al 2007). Regarding the effects of high-dose biotin on development and reproduction, Mittelholzer (1976) suggested that biotin at a dose totaling 50 mg/kg b.w.…”

Section: Introductionmentioning

confidence: 99%

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Dietary intake of high‐dose biotin inhibits spermatogenesis in young rats

Sawamura

Ikeda

Shimada

et al. 2015

Congenital Anomalies

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To characterize a new function of the watersoluble vitamin, biotin, in reproduction and early growth in mammals, the effects of high dietary doses of biotin on early spermatogenesis were biochemically and histologically investigated in male rats. Weaned rats were fed a CE-2 (control) diet containing 0.00004% biotin, or a control diet supplemented with 0.01%, 0.1%, or 1.0% biotin. Pair-fed rats were fed a control diet that was equal in calories to the amount ingested by the 1.0% biotin group, because food intake was decreased in the 1.0% biotin group. Food intake and body weight gain were lower in the 1.0% biotin group than in the control group. The kidney, brain and testis weights were significantly lower in the 1.0% biotin group than in the pair-fed group after 6 weeks of feeding. The accumulation of biotin in the liver and testis increased in a dose-dependent manner. In the 1.0% biotin group, the number of mature sperm was markedly lower, that of sperm with morphologically abnormal heads, mainly consisting of round heads, had increased. In addition, the development of seminiferous tubules was inhibited, and few spermatogonia and no spermatocytes were histologically observed. These results demonstrated that the long-term intake of high-dose biotin inhibited spermatogenesis in young male rats.

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“…Biotin is a cofactor for five mammalian carboxylases, and decreased activity of these leads to increased urinary excretion of 3-hydroxyisovalerate, 3-methylcrotonylglycine and other organic acids (Mock et al, 2004). It is also a cofactor for fatty acid synthesis, gluconeogenesis (Sawamura et al, 2007), and because it is intimately involved with epigenetic regulation of epithelial proliferation (O'Keefe et al, 2009), it has also been linked to the development of colorectal cancers. Biotin is a fermentation product of elements of the microbiome (Hill, 1997) and can also be derived from dietary protein.…”

Section: Microbial -Metabolic Correlationsmentioning

confidence: 99%

Neonatal environment exerts a sustained influence on the development of the intestinal microbiota and metabolic phenotype

et al. 2015

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The postnatal environment, including factors such as weaning and acquisition of the gut microbiota, has been causally linked to the development of later immunological diseases such as allergy and autoimmunity, and has also been associated with a predisposition to metabolic disorders. We show that the very early-life environment influences the development of both the gut microbiota and host metabolic phenotype in a porcine model of human infants. Farm piglets were nursed by their mothers for 1 day, before removal to highly controlled, individual isolators where they received formula milk until weaning at 21 days. The experiment was repeated, to create two batches, which differed only in minor environmental fluctuations during the first day. At day 1 after birth, metabolic profiling of serum by 1 H nuclear magnetic resonance spectroscopy demonstrated significant, systemic, inter-batch variation which persisted until weaning. However, the urinary metabolic profiles demonstrated that significant inter-batch effects on 3-hydroxyisovalerate, trimethylamine-N-oxide and mannitol persisted beyond weaning to at least 35 days. Batch effects were linked to significant differences in the composition of colonic microbiota at 35 days, determined by 16 S pyrosequencing. Different weaning diets modulated both the microbiota and metabolic phenotype independently of the persistent batch effects. We demonstrate that the environment during the first day of life influences development of the microbiota and metabolic phenotype and thus should be taken into account when interrogating experimental outcomes. In addition, we suggest that intervention at this early time could provide 'metabolic rescue' for at-risk infants who have undergone aberrant patterns of initial intestinal colonisation.

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Effects of Excess Biotin Administration on the Growth and Urinary Excretion of Water-Soluble Vitamins in Young Rats

Cited by 22 publications

References 31 publications

Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers

Dietary intake of high‐dose biotin inhibits spermatogenesis in young rats

Neonatal environment exerts a sustained influence on the development of the intestinal microbiota and metabolic phenotype

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